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The source of the fat significantly affects the results of high-fat diet intervention

High-fat diet (HFD) is widely used in animal models of many diseases, it helps to understand the pathogenic mechanism of related diseases. Several dietary fats were commonly used in HFD, such as corn oil, peanut oil, soybean oil, sunflower oil, and lard. However, it was reported that different dieta...

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Autores principales: An, Jiaxing, Wang, Qian, Yi, Suqin, Liu, Xuemei, Jin, Hai, Xu, Jingyu, Wen, Guorong, Zhu, Jiaxing, Tuo, Biguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918335/
https://www.ncbi.nlm.nih.gov/pubmed/35279685
http://dx.doi.org/10.1038/s41598-022-08249-2
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author An, Jiaxing
Wang, Qian
Yi, Suqin
Liu, Xuemei
Jin, Hai
Xu, Jingyu
Wen, Guorong
Zhu, Jiaxing
Tuo, Biguang
author_facet An, Jiaxing
Wang, Qian
Yi, Suqin
Liu, Xuemei
Jin, Hai
Xu, Jingyu
Wen, Guorong
Zhu, Jiaxing
Tuo, Biguang
author_sort An, Jiaxing
collection PubMed
description High-fat diet (HFD) is widely used in animal models of many diseases, it helps to understand the pathogenic mechanism of related diseases. Several dietary fats were commonly used in HFD, such as corn oil, peanut oil, soybean oil, sunflower oil, and lard. However, it was reported that different dietary fat could have completely different effects on physiological indicators and the gut microbiome, and the sources of dietary fat used in high-fat diet research have not been comprehensively compared. In this research, we conduct comparative experiments on various sources of dietary fats to test their different effects during the high-fat diet intervention. We investigated the effects of twelve common dietary fats in high-fat diet intervention of mice, body/liver weight changes, four blood lipid indices, and gut microbiome were analyzed. Our results showed that the source of dietary fat used in high-fat diet significantly affects the changes of body/liver weight and triglyceride (TRIG) in the blood. Furthermore, the intervention of canola oil increased the alpha diversity of gut microbiota, and lard has decreased diversity compared with the control group. The composition of saturated fatty acid (SFA) in fat has the most significant effects on the gut microbiome. All dietary fats treatments have an increasing Firmicutes abundance and a reduced Bacteroidetes abundance in gut microbiome, while the canola oil has a slight variation compared to other intervention groups, and the lard group has the largest changes. This study showed that different types of dietary fat have different effects on the body indicators and intestinal microbiota of mice, and canola oil produced less disturbance than other types of dietary fats in high-fat diet.
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spelling pubmed-89183352022-03-16 The source of the fat significantly affects the results of high-fat diet intervention An, Jiaxing Wang, Qian Yi, Suqin Liu, Xuemei Jin, Hai Xu, Jingyu Wen, Guorong Zhu, Jiaxing Tuo, Biguang Sci Rep Article High-fat diet (HFD) is widely used in animal models of many diseases, it helps to understand the pathogenic mechanism of related diseases. Several dietary fats were commonly used in HFD, such as corn oil, peanut oil, soybean oil, sunflower oil, and lard. However, it was reported that different dietary fat could have completely different effects on physiological indicators and the gut microbiome, and the sources of dietary fat used in high-fat diet research have not been comprehensively compared. In this research, we conduct comparative experiments on various sources of dietary fats to test their different effects during the high-fat diet intervention. We investigated the effects of twelve common dietary fats in high-fat diet intervention of mice, body/liver weight changes, four blood lipid indices, and gut microbiome were analyzed. Our results showed that the source of dietary fat used in high-fat diet significantly affects the changes of body/liver weight and triglyceride (TRIG) in the blood. Furthermore, the intervention of canola oil increased the alpha diversity of gut microbiota, and lard has decreased diversity compared with the control group. The composition of saturated fatty acid (SFA) in fat has the most significant effects on the gut microbiome. All dietary fats treatments have an increasing Firmicutes abundance and a reduced Bacteroidetes abundance in gut microbiome, while the canola oil has a slight variation compared to other intervention groups, and the lard group has the largest changes. This study showed that different types of dietary fat have different effects on the body indicators and intestinal microbiota of mice, and canola oil produced less disturbance than other types of dietary fats in high-fat diet. Nature Publishing Group UK 2022-03-12 /pmc/articles/PMC8918335/ /pubmed/35279685 http://dx.doi.org/10.1038/s41598-022-08249-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
An, Jiaxing
Wang, Qian
Yi, Suqin
Liu, Xuemei
Jin, Hai
Xu, Jingyu
Wen, Guorong
Zhu, Jiaxing
Tuo, Biguang
The source of the fat significantly affects the results of high-fat diet intervention
title The source of the fat significantly affects the results of high-fat diet intervention
title_full The source of the fat significantly affects the results of high-fat diet intervention
title_fullStr The source of the fat significantly affects the results of high-fat diet intervention
title_full_unstemmed The source of the fat significantly affects the results of high-fat diet intervention
title_short The source of the fat significantly affects the results of high-fat diet intervention
title_sort source of the fat significantly affects the results of high-fat diet intervention
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918335/
https://www.ncbi.nlm.nih.gov/pubmed/35279685
http://dx.doi.org/10.1038/s41598-022-08249-2
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