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Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis
Current clinical treatment targeting osteosarcoma (OS) are limited for OS patients with pulmonary metastasis or relapse, which led to high mortality (70%‐85%) for advanced osteosarcoma patients. Although ongoing efforts have been made to illustrate the mechanisms of tumorigenesis and progression in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918411/ https://www.ncbi.nlm.nih.gov/pubmed/33621401 http://dx.doi.org/10.1111/jcmm.16166 |
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author | Zhang, Meng Yu, Guang‐Yang Liu, Gang Liu, Wei‐Dong |
author_facet | Zhang, Meng Yu, Guang‐Yang Liu, Gang Liu, Wei‐Dong |
author_sort | Zhang, Meng |
collection | PubMed |
description | Current clinical treatment targeting osteosarcoma (OS) are limited for OS patients with pulmonary metastasis or relapse, which led to high mortality (70%‐85%) for advanced osteosarcoma patients. Although ongoing efforts have been made to illustrate the mechanisms of tumorigenesis and progression in OS; however, it was far for us to learn a comprehensive molecular mechanism implies in OS development. In our study, we implicated a circRNA hsa_circ_0002137, which was higher expressed in osteosarcoma tumours compared with paracancerous tissue. The dysregulated expression pattern was also found in osteosarcoma cell lines. The role of circ_0002137 was explored via down‐ or up‐regulated experiments. It was proved that down‐regulation of circ_0002137 suppressed the progress of OS, including cell invasion, cell cycle and cell apoptosis. Furthermore, the correlation between circ_0002137 and miR‐433‐3p was predicted using bioinformatic tools and verified utilizing RNA pull‐down assay and luciferase reporter assay. Interestingly, we found that the inhibitory effect of circ_0002137 on OS was dependent of insulin‐like growth factor‐1 receptor (IGF1R). In conclusion, it was demonstrated that circ_0002137 could restrain the progression of OS through regulating miR‐433‐3p/IGF1R axis, providing a comprehensive landscape of circ_0002137 in the generation and development of OS. |
format | Online Article Text |
id | pubmed-8918411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89184112022-03-18 Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis Zhang, Meng Yu, Guang‐Yang Liu, Gang Liu, Wei‐Dong J Cell Mol Med Original Articles Current clinical treatment targeting osteosarcoma (OS) are limited for OS patients with pulmonary metastasis or relapse, which led to high mortality (70%‐85%) for advanced osteosarcoma patients. Although ongoing efforts have been made to illustrate the mechanisms of tumorigenesis and progression in OS; however, it was far for us to learn a comprehensive molecular mechanism implies in OS development. In our study, we implicated a circRNA hsa_circ_0002137, which was higher expressed in osteosarcoma tumours compared with paracancerous tissue. The dysregulated expression pattern was also found in osteosarcoma cell lines. The role of circ_0002137 was explored via down‐ or up‐regulated experiments. It was proved that down‐regulation of circ_0002137 suppressed the progress of OS, including cell invasion, cell cycle and cell apoptosis. Furthermore, the correlation between circ_0002137 and miR‐433‐3p was predicted using bioinformatic tools and verified utilizing RNA pull‐down assay and luciferase reporter assay. Interestingly, we found that the inhibitory effect of circ_0002137 on OS was dependent of insulin‐like growth factor‐1 receptor (IGF1R). In conclusion, it was demonstrated that circ_0002137 could restrain the progression of OS through regulating miR‐433‐3p/IGF1R axis, providing a comprehensive landscape of circ_0002137 in the generation and development of OS. John Wiley and Sons Inc. 2021-02-23 2022-03 /pmc/articles/PMC8918411/ /pubmed/33621401 http://dx.doi.org/10.1111/jcmm.16166 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zhang, Meng Yu, Guang‐Yang Liu, Gang Liu, Wei‐Dong Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title | Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title_full | Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title_fullStr | Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title_full_unstemmed | Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title_short | Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR‐433‐3p/ IGF1R axis |
title_sort | circular rna circ_0002137 regulated the progression of osteosarcoma through regulating mir‐433‐3p/ igf1r axis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918411/ https://www.ncbi.nlm.nih.gov/pubmed/33621401 http://dx.doi.org/10.1111/jcmm.16166 |
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