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Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms

Many Pseudoalteromonas species are dominant biofilm-forming Gammaproteobacteria in the ocean. The formation of Pseudoalteromonas biofilms is often accompanied by the occurrence of variants with different colony morphologies that may exhibit increased marine antifouling or anticorrosion activities. H...

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Autores principales: Zeng, Zhenshun, Lin, Shituan, Li, Qian, Wang, Weiquan, Wang, Yuqi, Xiao, Tangfu, Guo, Yuexue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919034/
https://www.ncbi.nlm.nih.gov/pubmed/35295294
http://dx.doi.org/10.3389/fmicb.2022.797197
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author Zeng, Zhenshun
Lin, Shituan
Li, Qian
Wang, Weiquan
Wang, Yuqi
Xiao, Tangfu
Guo, Yuexue
author_facet Zeng, Zhenshun
Lin, Shituan
Li, Qian
Wang, Weiquan
Wang, Yuqi
Xiao, Tangfu
Guo, Yuexue
author_sort Zeng, Zhenshun
collection PubMed
description Many Pseudoalteromonas species are dominant biofilm-forming Gammaproteobacteria in the ocean. The formation of Pseudoalteromonas biofilms is often accompanied by the occurrence of variants with different colony morphologies that may exhibit increased marine antifouling or anticorrosion activities. However, the genetic basis of the occurrence of these variants remains largely unexplored. In this study, we identified that wrinkled variants of P. lipolytica mainly arose due to mutations in the AT00_08765, a wspF-like gene, that are associated with decreased swimming motility and increased cellulose production. Moreover, we found that the spontaneous mutation in flhA, encoding a flagellar biosynthesis protein, also caused a wrinkled colony morphology that is associated with cellulose overproduction, indicating that flhA plays a dual role in controlling flagellar assembly and polysaccharide production in P. lipolytica. Investigation of wrinkled variants harboring spontaneous mutation in dgcB, encoding a GGDEF domain protein, also demonstrated dgcB plays an important role in regulating cellulose production and swimming motility. In addition, by screening the suppressor of the AT00_08765 variant strain, we also identified that the spontaneous mutation in cheR and bcsC directly abolished the wrinkled phenotype of the AT00_08765 variant strain, suggesting that the chemosensory signaling transduction and cellulose production are crucial for the determination of the wrinkled phenotype in P. lipolytica. Taken together, this study provides insights into the genetic variation within biofilms of P. lipolytica.
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spelling pubmed-89190342022-03-15 Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms Zeng, Zhenshun Lin, Shituan Li, Qian Wang, Weiquan Wang, Yuqi Xiao, Tangfu Guo, Yuexue Front Microbiol Microbiology Many Pseudoalteromonas species are dominant biofilm-forming Gammaproteobacteria in the ocean. The formation of Pseudoalteromonas biofilms is often accompanied by the occurrence of variants with different colony morphologies that may exhibit increased marine antifouling or anticorrosion activities. However, the genetic basis of the occurrence of these variants remains largely unexplored. In this study, we identified that wrinkled variants of P. lipolytica mainly arose due to mutations in the AT00_08765, a wspF-like gene, that are associated with decreased swimming motility and increased cellulose production. Moreover, we found that the spontaneous mutation in flhA, encoding a flagellar biosynthesis protein, also caused a wrinkled colony morphology that is associated with cellulose overproduction, indicating that flhA plays a dual role in controlling flagellar assembly and polysaccharide production in P. lipolytica. Investigation of wrinkled variants harboring spontaneous mutation in dgcB, encoding a GGDEF domain protein, also demonstrated dgcB plays an important role in regulating cellulose production and swimming motility. In addition, by screening the suppressor of the AT00_08765 variant strain, we also identified that the spontaneous mutation in cheR and bcsC directly abolished the wrinkled phenotype of the AT00_08765 variant strain, suggesting that the chemosensory signaling transduction and cellulose production are crucial for the determination of the wrinkled phenotype in P. lipolytica. Taken together, this study provides insights into the genetic variation within biofilms of P. lipolytica. Frontiers Media S.A. 2022-02-28 /pmc/articles/PMC8919034/ /pubmed/35295294 http://dx.doi.org/10.3389/fmicb.2022.797197 Text en Copyright © 2022 Zeng, Lin, Li, Wang, Wang, Xiao and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zeng, Zhenshun
Lin, Shituan
Li, Qian
Wang, Weiquan
Wang, Yuqi
Xiao, Tangfu
Guo, Yuexue
Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title_full Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title_fullStr Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title_full_unstemmed Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title_short Molecular Basis of Wrinkled Variants Isolated From Pseudoalteromonas lipolytica Biofilms
title_sort molecular basis of wrinkled variants isolated from pseudoalteromonas lipolytica biofilms
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919034/
https://www.ncbi.nlm.nih.gov/pubmed/35295294
http://dx.doi.org/10.3389/fmicb.2022.797197
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