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Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis
BACKGROUND: Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. RESULTS: Herein, investigations revealed that chilling and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919617/ https://www.ncbi.nlm.nih.gov/pubmed/35287582 http://dx.doi.org/10.1186/s12870-022-03507-x |
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author | Zhu, Shanshan Gu, Denghui Lu, Caiping Zhang, Caixia Chen, Juanjuan Yang, Rui Luo, Qijun Wang, Tiegan Zhang, Peng Chen, Haimin |
author_facet | Zhu, Shanshan Gu, Denghui Lu, Caiping Zhang, Caixia Chen, Juanjuan Yang, Rui Luo, Qijun Wang, Tiegan Zhang, Peng Chen, Haimin |
author_sort | Zhu, Shanshan |
collection | PubMed |
description | BACKGROUND: Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. RESULTS: Herein, investigations revealed that chilling and freezing induced alterations in the physiological properties, gene transcriptional profiles and metabolite levels in the economically important red algae species, Neoporphyra haitanensis. Control samples (kept at 20 °C) were compared to chilled thalli (10 and 4 °C) and to thalli under − 4 °C conditions. Chilling stress did not affect the health or photosynthetic efficiency of gametophytes, but freezing conditions resulted in the arrest of growth, death of some cells and a decrease in photosynthetic activity as calculated by Fv/Fm. Transcriptome sequencing analysis revealed that the photosynthetic system was down-regulated along with genes associated with carbon fixation and primary metabolic biosynthesis. Adaptive mechanisms included an increase in unsaturated fatty acids levels to improve membrane fluidity, an increase in floridoside and isofloridoside content to enhance osmotic resistance, and an elevation in levels of some resistance-associated phytohormones (abscisic acid, salicylic acid, and methyl jasmonic acid). These physiochemical alterations occurred together with the upregulation of ribosome biogenesis. CONCLUSIONS: N. haitanensis adopts multiple protective mechanisms to maintain homeostasis of cellular physiology in tolerance to cold stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03507-x. |
format | Online Article Text |
id | pubmed-8919617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-89196172022-03-16 Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis Zhu, Shanshan Gu, Denghui Lu, Caiping Zhang, Caixia Chen, Juanjuan Yang, Rui Luo, Qijun Wang, Tiegan Zhang, Peng Chen, Haimin BMC Plant Biol Research BACKGROUND: Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. RESULTS: Herein, investigations revealed that chilling and freezing induced alterations in the physiological properties, gene transcriptional profiles and metabolite levels in the economically important red algae species, Neoporphyra haitanensis. Control samples (kept at 20 °C) were compared to chilled thalli (10 and 4 °C) and to thalli under − 4 °C conditions. Chilling stress did not affect the health or photosynthetic efficiency of gametophytes, but freezing conditions resulted in the arrest of growth, death of some cells and a decrease in photosynthetic activity as calculated by Fv/Fm. Transcriptome sequencing analysis revealed that the photosynthetic system was down-regulated along with genes associated with carbon fixation and primary metabolic biosynthesis. Adaptive mechanisms included an increase in unsaturated fatty acids levels to improve membrane fluidity, an increase in floridoside and isofloridoside content to enhance osmotic resistance, and an elevation in levels of some resistance-associated phytohormones (abscisic acid, salicylic acid, and methyl jasmonic acid). These physiochemical alterations occurred together with the upregulation of ribosome biogenesis. CONCLUSIONS: N. haitanensis adopts multiple protective mechanisms to maintain homeostasis of cellular physiology in tolerance to cold stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03507-x. BioMed Central 2022-03-14 /pmc/articles/PMC8919617/ /pubmed/35287582 http://dx.doi.org/10.1186/s12870-022-03507-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhu, Shanshan Gu, Denghui Lu, Caiping Zhang, Caixia Chen, Juanjuan Yang, Rui Luo, Qijun Wang, Tiegan Zhang, Peng Chen, Haimin Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title_full | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title_fullStr | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title_full_unstemmed | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title_short | Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis |
title_sort | cold stress tolerance of the intertidal red alga neoporphyra haitanensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919617/ https://www.ncbi.nlm.nih.gov/pubmed/35287582 http://dx.doi.org/10.1186/s12870-022-03507-x |
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