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BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity
OBJECTIVES: Mesenchymal stem cells (MSCs) have become seed cells and basic elements for bone regeneration and bone tissue engineering. The aim of the present study was to investigate the roles and mechanisms of bone morphogenetic protein 2 (BMP-2) on osteogenic differentiation of MSCs. METHODS: Prim...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Society of Musculoskeletal and Neuronal Interactions
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919656/ https://www.ncbi.nlm.nih.gov/pubmed/35234167 |
_version_ | 1784668978006720512 |
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author | Li, Yinan Fu, Guangmin Gong, Yahui Li, Bo Li, Wei Liu, Dacheng Yang, Xiaoning |
author_facet | Li, Yinan Fu, Guangmin Gong, Yahui Li, Bo Li, Wei Liu, Dacheng Yang, Xiaoning |
author_sort | Li, Yinan |
collection | PubMed |
description | OBJECTIVES: Mesenchymal stem cells (MSCs) have become seed cells and basic elements for bone regeneration and bone tissue engineering. The aim of the present study was to investigate the roles and mechanisms of bone morphogenetic protein 2 (BMP-2) on osteogenic differentiation of MSCs. METHODS: Primary MSCs were isolated from the femur and tibia bone of rats and then transfected with BMP-2 and PGC-1α adenovirus vectors. Alkaline phosphatase (ALP) activity and alizarin red staining were used to measure osteogenic differentiation of MSCs. Real-time PCR and western blot assays were performed to assess osteogenic differentiation-related proteins levels. The activities of mitochondrial respiratory chain complexes I and II and mitochondrial fluorescence intensity were used to explore mitochondria status during osteogenic differentiation of MSCs. RESULTS: We found that the ability of BMP-2 overexpressed (OE) group osteogenic differentiation was significantly improved, compared with the negative control (NC) group. The results also indicated that BMP-2 can promote the activity of mitochondria. We further used the gain- and loss-of-function approaches to demonstrate that BMP-2 promotes mitochondrial activity by up-regulating PGC-1α to promote osteogenic differentiation of MSCs. CONCLUSIONS: These results explored the important role of BMP-2 in the osteoblast differentiation of MSCs from a new perspective, providing a theoretical and experimental basis for bone defect and repair. |
format | Online Article Text |
id | pubmed-8919656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Society of Musculoskeletal and Neuronal Interactions |
record_format | MEDLINE/PubMed |
spelling | pubmed-89196562022-04-14 BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity Li, Yinan Fu, Guangmin Gong, Yahui Li, Bo Li, Wei Liu, Dacheng Yang, Xiaoning J Musculoskelet Neuronal Interact Original Article OBJECTIVES: Mesenchymal stem cells (MSCs) have become seed cells and basic elements for bone regeneration and bone tissue engineering. The aim of the present study was to investigate the roles and mechanisms of bone morphogenetic protein 2 (BMP-2) on osteogenic differentiation of MSCs. METHODS: Primary MSCs were isolated from the femur and tibia bone of rats and then transfected with BMP-2 and PGC-1α adenovirus vectors. Alkaline phosphatase (ALP) activity and alizarin red staining were used to measure osteogenic differentiation of MSCs. Real-time PCR and western blot assays were performed to assess osteogenic differentiation-related proteins levels. The activities of mitochondrial respiratory chain complexes I and II and mitochondrial fluorescence intensity were used to explore mitochondria status during osteogenic differentiation of MSCs. RESULTS: We found that the ability of BMP-2 overexpressed (OE) group osteogenic differentiation was significantly improved, compared with the negative control (NC) group. The results also indicated that BMP-2 can promote the activity of mitochondria. We further used the gain- and loss-of-function approaches to demonstrate that BMP-2 promotes mitochondrial activity by up-regulating PGC-1α to promote osteogenic differentiation of MSCs. CONCLUSIONS: These results explored the important role of BMP-2 in the osteoblast differentiation of MSCs from a new perspective, providing a theoretical and experimental basis for bone defect and repair. International Society of Musculoskeletal and Neuronal Interactions 2022 /pmc/articles/PMC8919656/ /pubmed/35234167 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Li, Yinan Fu, Guangmin Gong, Yahui Li, Bo Li, Wei Liu, Dacheng Yang, Xiaoning BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title | BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title_full | BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title_fullStr | BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title_full_unstemmed | BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title_short | BMP-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
title_sort | bmp-2 promotes osteogenic differentiation of mesenchymal stem cells by enhancing mitochondrial activity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919656/ https://www.ncbi.nlm.nih.gov/pubmed/35234167 |
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