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Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord

After spinal cord injury, gliomesenchymal scaring inhibits axonal regeneration as a physical barrier. In peripheral nerve injuries, native spider silk was shown to be an effective scaffold to facilitate axonal re-growth and nerve regeneration. This study tested a two-composite scaffold made of longi...

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Autores principales: Koop, Felix, Strauß, Sarah, Peck, Claas-Tido, Aper, Thomas, Wilhelmi, Mathias, Hartmann, Christian, Hegermann, Jan, Schipke, Julia, Vogt, Peter M., Bucan, Vesna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920256/
https://www.ncbi.nlm.nih.gov/pubmed/35286342
http://dx.doi.org/10.1371/journal.pone.0264486
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author Koop, Felix
Strauß, Sarah
Peck, Claas-Tido
Aper, Thomas
Wilhelmi, Mathias
Hartmann, Christian
Hegermann, Jan
Schipke, Julia
Vogt, Peter M.
Bucan, Vesna
author_facet Koop, Felix
Strauß, Sarah
Peck, Claas-Tido
Aper, Thomas
Wilhelmi, Mathias
Hartmann, Christian
Hegermann, Jan
Schipke, Julia
Vogt, Peter M.
Bucan, Vesna
author_sort Koop, Felix
collection PubMed
description After spinal cord injury, gliomesenchymal scaring inhibits axonal regeneration as a physical barrier. In peripheral nerve injuries, native spider silk was shown to be an effective scaffold to facilitate axonal re-growth and nerve regeneration. This study tested a two-composite scaffold made of longitudinally oriented native spider silk containing a Haemocomplettan fibrin sheath to bridge lesions in the spinal cord and enhance axonal sprouting. In vitro cultivation of neuronal cells on spider silk and fibrin revealed no cytotoxicity of the scaffold components. When spinal cord tissue was cultured on spider silk that was reeled around a metal frame, migration of different cell types, including neurons and neural stem cells, was observed. The scaffold was implanted into spinal cord lesions of four Wistar rats to evaluate the physical stress caused on the animals and examine the bridging potential for axonal sprouting and spinal cord regeneration. However, the implantation in-vivo resulted in a granulomatous foreign body reaction. Spider silk might be responsible for the strong immune response. Thus, the immune response to native spider silk seems to be stronger in the central nervous system than it is known to be in the peripheral body complicating the application of native spider silk in spinal cord injury treatment.
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spelling pubmed-89202562022-03-15 Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord Koop, Felix Strauß, Sarah Peck, Claas-Tido Aper, Thomas Wilhelmi, Mathias Hartmann, Christian Hegermann, Jan Schipke, Julia Vogt, Peter M. Bucan, Vesna PLoS One Research Article After spinal cord injury, gliomesenchymal scaring inhibits axonal regeneration as a physical barrier. In peripheral nerve injuries, native spider silk was shown to be an effective scaffold to facilitate axonal re-growth and nerve regeneration. This study tested a two-composite scaffold made of longitudinally oriented native spider silk containing a Haemocomplettan fibrin sheath to bridge lesions in the spinal cord and enhance axonal sprouting. In vitro cultivation of neuronal cells on spider silk and fibrin revealed no cytotoxicity of the scaffold components. When spinal cord tissue was cultured on spider silk that was reeled around a metal frame, migration of different cell types, including neurons and neural stem cells, was observed. The scaffold was implanted into spinal cord lesions of four Wistar rats to evaluate the physical stress caused on the animals and examine the bridging potential for axonal sprouting and spinal cord regeneration. However, the implantation in-vivo resulted in a granulomatous foreign body reaction. Spider silk might be responsible for the strong immune response. Thus, the immune response to native spider silk seems to be stronger in the central nervous system than it is known to be in the peripheral body complicating the application of native spider silk in spinal cord injury treatment. Public Library of Science 2022-03-14 /pmc/articles/PMC8920256/ /pubmed/35286342 http://dx.doi.org/10.1371/journal.pone.0264486 Text en © 2022 Koop et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Koop, Felix
Strauß, Sarah
Peck, Claas-Tido
Aper, Thomas
Wilhelmi, Mathias
Hartmann, Christian
Hegermann, Jan
Schipke, Julia
Vogt, Peter M.
Bucan, Vesna
Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title_full Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title_fullStr Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title_full_unstemmed Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title_short Preliminary application of native Nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
title_sort preliminary application of native nephila edulis spider silk and fibrin implant causes granulomatous foreign body reaction in vivo in rat’s spinal cord
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920256/
https://www.ncbi.nlm.nih.gov/pubmed/35286342
http://dx.doi.org/10.1371/journal.pone.0264486
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