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Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice

Recently food shortage has become the major flagging scenario around the globe. To resolve this challenge, there is dire need to significantly increase crop productivity per unit area. In the present study, 24 genotypes of rice were grown in pots to assess their tillering number, number of primary a...

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Autores principales: Din, Muhammad Salah ud, Wang, Xiukang, Alamery, Salman, Fiaz, Sajid, Rasheed, Haroon, Khan, Muhammad Abid, Khan, Shahid Ullah, Saeed, Sumbul, Ali, Niaz, Marwat, Kalim Ullah, Attia, Kotb, Kimiko, Itoh, Wani, Shabir Hussain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920263/
https://www.ncbi.nlm.nih.gov/pubmed/35286332
http://dx.doi.org/10.1371/journal.pone.0264478
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author Din, Muhammad Salah ud
Wang, Xiukang
Alamery, Salman
Fiaz, Sajid
Rasheed, Haroon
Khan, Muhammad Abid
Khan, Shahid Ullah
Saeed, Sumbul
Ali, Niaz
Marwat, Kalim Ullah
Attia, Kotb
Kimiko, Itoh
Wani, Shabir Hussain
author_facet Din, Muhammad Salah ud
Wang, Xiukang
Alamery, Salman
Fiaz, Sajid
Rasheed, Haroon
Khan, Muhammad Abid
Khan, Shahid Ullah
Saeed, Sumbul
Ali, Niaz
Marwat, Kalim Ullah
Attia, Kotb
Kimiko, Itoh
Wani, Shabir Hussain
author_sort Din, Muhammad Salah ud
collection PubMed
description Recently food shortage has become the major flagging scenario around the globe. To resolve this challenge, there is dire need to significantly increase crop productivity per unit area. In the present study, 24 genotypes of rice were grown in pots to assess their tillering number, number of primary and secondary branches per panicle, number of grains per panicle, number of grains per plant, and grain yield, respectively. In addition, the potential function of miR156 was analyzed, regulating seed sequence in rice. Furthermore, OsSPL14 gene for miR156 was sequenced to identify additional mutations within studied region. The results demonstrated Bas-370 and L-77 showed highest and lowest tillers, respectively. Bas-370, Rachna basmati, Bas-2000, and Kashmir Basmati showed high panicle branches whereas, L-77, L-46, Dilrosh, L-48, and L-20 displayed lowest panicle branches. Bas-370 and four other studied accessions contained C allele whereas, L-77 and 18 other investigated accessions had heterozygous (C and T) alleles in their promoter region. C-T allelic mutation was found in 3(rd) exon of the OsSPL14 gene. The sequence analysis of 12 accessions revealed a novel mutation (C-T) present ~2bp upstream and substitution of C-A allele. However, no significant correlation for novel mutation was found for tillering and panicle branches in studied rice accessions. Taken together present results suggested novel insight into the binding of miR156 to detected mutation found in 3(rd) exon of the OsSPL14 gene. Nevertheless, L-77, L-46, Dilrosh, L-48, and L-20 could be used as potential breeding resource for improving panicle architecture contributing yield improvement of rice crop.
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spelling pubmed-89202632022-03-15 Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice Din, Muhammad Salah ud Wang, Xiukang Alamery, Salman Fiaz, Sajid Rasheed, Haroon Khan, Muhammad Abid Khan, Shahid Ullah Saeed, Sumbul Ali, Niaz Marwat, Kalim Ullah Attia, Kotb Kimiko, Itoh Wani, Shabir Hussain PLoS One Research Article Recently food shortage has become the major flagging scenario around the globe. To resolve this challenge, there is dire need to significantly increase crop productivity per unit area. In the present study, 24 genotypes of rice were grown in pots to assess their tillering number, number of primary and secondary branches per panicle, number of grains per panicle, number of grains per plant, and grain yield, respectively. In addition, the potential function of miR156 was analyzed, regulating seed sequence in rice. Furthermore, OsSPL14 gene for miR156 was sequenced to identify additional mutations within studied region. The results demonstrated Bas-370 and L-77 showed highest and lowest tillers, respectively. Bas-370, Rachna basmati, Bas-2000, and Kashmir Basmati showed high panicle branches whereas, L-77, L-46, Dilrosh, L-48, and L-20 displayed lowest panicle branches. Bas-370 and four other studied accessions contained C allele whereas, L-77 and 18 other investigated accessions had heterozygous (C and T) alleles in their promoter region. C-T allelic mutation was found in 3(rd) exon of the OsSPL14 gene. The sequence analysis of 12 accessions revealed a novel mutation (C-T) present ~2bp upstream and substitution of C-A allele. However, no significant correlation for novel mutation was found for tillering and panicle branches in studied rice accessions. Taken together present results suggested novel insight into the binding of miR156 to detected mutation found in 3(rd) exon of the OsSPL14 gene. Nevertheless, L-77, L-46, Dilrosh, L-48, and L-20 could be used as potential breeding resource for improving panicle architecture contributing yield improvement of rice crop. Public Library of Science 2022-03-14 /pmc/articles/PMC8920263/ /pubmed/35286332 http://dx.doi.org/10.1371/journal.pone.0264478 Text en © 2022 Din et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Din, Muhammad Salah ud
Wang, Xiukang
Alamery, Salman
Fiaz, Sajid
Rasheed, Haroon
Khan, Muhammad Abid
Khan, Shahid Ullah
Saeed, Sumbul
Ali, Niaz
Marwat, Kalim Ullah
Attia, Kotb
Kimiko, Itoh
Wani, Shabir Hussain
Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title_full Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title_fullStr Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title_full_unstemmed Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title_short Identification of C-T novel polymorphism in 3(rd) exon of OsSPL14 gene governing seed sequence in rice
title_sort identification of c-t novel polymorphism in 3(rd) exon of osspl14 gene governing seed sequence in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920263/
https://www.ncbi.nlm.nih.gov/pubmed/35286332
http://dx.doi.org/10.1371/journal.pone.0264478
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