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Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi

Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated th...

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Autores principales: Matušinsky, Pavel, Sedláková, Božena, Bleša, Dominik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920291/
https://www.ncbi.nlm.nih.gov/pubmed/35286339
http://dx.doi.org/10.1371/journal.pone.0265357
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author Matušinsky, Pavel
Sedláková, Božena
Bleša, Dominik
author_facet Matušinsky, Pavel
Sedláková, Božena
Bleša, Dominik
author_sort Matušinsky, Pavel
collection PubMed
description Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi.
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spelling pubmed-89202912022-03-15 Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi Matušinsky, Pavel Sedláková, Božena Bleša, Dominik PLoS One Research Article Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi. Public Library of Science 2022-03-14 /pmc/articles/PMC8920291/ /pubmed/35286339 http://dx.doi.org/10.1371/journal.pone.0265357 Text en © 2022 Matušinsky et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Matušinsky, Pavel
Sedláková, Božena
Bleša, Dominik
Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title_full Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title_fullStr Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title_full_unstemmed Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title_short Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
title_sort compatible interaction of brachypodium distachyon and endophytic fungus microdochium bolleyi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920291/
https://www.ncbi.nlm.nih.gov/pubmed/35286339
http://dx.doi.org/10.1371/journal.pone.0265357
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