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Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920291/ https://www.ncbi.nlm.nih.gov/pubmed/35286339 http://dx.doi.org/10.1371/journal.pone.0265357 |
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author | Matušinsky, Pavel Sedláková, Božena Bleša, Dominik |
author_facet | Matušinsky, Pavel Sedláková, Božena Bleša, Dominik |
author_sort | Matušinsky, Pavel |
collection | PubMed |
description | Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi. |
format | Online Article Text |
id | pubmed-8920291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89202912022-03-15 Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi Matušinsky, Pavel Sedláková, Božena Bleša, Dominik PLoS One Research Article Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi. Public Library of Science 2022-03-14 /pmc/articles/PMC8920291/ /pubmed/35286339 http://dx.doi.org/10.1371/journal.pone.0265357 Text en © 2022 Matušinsky et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Matušinsky, Pavel Sedláková, Božena Bleša, Dominik Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title | Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title_full | Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title_fullStr | Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title_full_unstemmed | Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title_short | Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi |
title_sort | compatible interaction of brachypodium distachyon and endophytic fungus microdochium bolleyi |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920291/ https://www.ncbi.nlm.nih.gov/pubmed/35286339 http://dx.doi.org/10.1371/journal.pone.0265357 |
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