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Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection

Despite the availability of licensed vaccines, pneumococcal disease caused by the bacteria Streptococcus pneumoniae (pneumococcus), remains a serious infectious disease threat globally. Disease manifestations include pneumonia, bacteremia, and meningitis, resulting in over a million deaths annually....

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Autores principales: Bhalla, Manmeet, Nayerhoda, Roozbeh, Tchalla, Essi Y. I., Abamonte, Alexsandra, Park, Dongwon, Simmons, Shaunna R., Pfeifer, Blaine A., Bou Ghanem, Elsa N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920316/
https://www.ncbi.nlm.nih.gov/pubmed/35291600
http://dx.doi.org/10.3389/fragi.2021.798868
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author Bhalla, Manmeet
Nayerhoda, Roozbeh
Tchalla, Essi Y. I.
Abamonte, Alexsandra
Park, Dongwon
Simmons, Shaunna R.
Pfeifer, Blaine A.
Bou Ghanem, Elsa N.
author_facet Bhalla, Manmeet
Nayerhoda, Roozbeh
Tchalla, Essi Y. I.
Abamonte, Alexsandra
Park, Dongwon
Simmons, Shaunna R.
Pfeifer, Blaine A.
Bou Ghanem, Elsa N.
author_sort Bhalla, Manmeet
collection PubMed
description Despite the availability of licensed vaccines, pneumococcal disease caused by the bacteria Streptococcus pneumoniae (pneumococcus), remains a serious infectious disease threat globally. Disease manifestations include pneumonia, bacteremia, and meningitis, resulting in over a million deaths annually. Pneumococcal disease disproportionally impacts older adults aged ≥65 years. Interventions are complicated through a combination of complex disease progression and 100 different bacterial capsular polysaccharide serotypes. This has made it challenging to develop a broad vaccine against S. pneumoniae, with current options utilizing capsular polysaccharides as the primary antigenic content. However, current vaccines are substantially less effective in protecting the elderly. We previously developed a Liposomal Encapsulation of Polysaccharides (LEPS) vaccine platform, designed around limitations of current pneumococcal vaccines, that allowed the non-covalent coupling of polysaccharide and protein antigen content and protected young hosts against pneumococcal infection in murine models. In this study, we modified the formulation to make it more economical and tested the novel LEPS vaccine in aged hosts. We found that in young mice (2–3 months), LEPS elicited comparable responses to the pneumococcal conjugate vaccine Prevnar-13. Further, LEPS immunization of old mice (18–22 months) induced comparable antibody levels and improved antibody function compared to Prevnar-13. Importantly, LEPS protected old mice against both invasive and lung localized pneumococcal infections. In summary, LEPS is an alternative and effective vaccine strategy that protects aged hosts against different manifestations of pneumococcal disease.
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spelling pubmed-89203162022-03-14 Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection Bhalla, Manmeet Nayerhoda, Roozbeh Tchalla, Essi Y. I. Abamonte, Alexsandra Park, Dongwon Simmons, Shaunna R. Pfeifer, Blaine A. Bou Ghanem, Elsa N. Front Aging Aging Despite the availability of licensed vaccines, pneumococcal disease caused by the bacteria Streptococcus pneumoniae (pneumococcus), remains a serious infectious disease threat globally. Disease manifestations include pneumonia, bacteremia, and meningitis, resulting in over a million deaths annually. Pneumococcal disease disproportionally impacts older adults aged ≥65 years. Interventions are complicated through a combination of complex disease progression and 100 different bacterial capsular polysaccharide serotypes. This has made it challenging to develop a broad vaccine against S. pneumoniae, with current options utilizing capsular polysaccharides as the primary antigenic content. However, current vaccines are substantially less effective in protecting the elderly. We previously developed a Liposomal Encapsulation of Polysaccharides (LEPS) vaccine platform, designed around limitations of current pneumococcal vaccines, that allowed the non-covalent coupling of polysaccharide and protein antigen content and protected young hosts against pneumococcal infection in murine models. In this study, we modified the formulation to make it more economical and tested the novel LEPS vaccine in aged hosts. We found that in young mice (2–3 months), LEPS elicited comparable responses to the pneumococcal conjugate vaccine Prevnar-13. Further, LEPS immunization of old mice (18–22 months) induced comparable antibody levels and improved antibody function compared to Prevnar-13. Importantly, LEPS protected old mice against both invasive and lung localized pneumococcal infections. In summary, LEPS is an alternative and effective vaccine strategy that protects aged hosts against different manifestations of pneumococcal disease. Frontiers Media S.A. 2021-12-22 /pmc/articles/PMC8920316/ /pubmed/35291600 http://dx.doi.org/10.3389/fragi.2021.798868 Text en Copyright © 2021 Bhalla, Nayerhoda, Tchalla, Abamonte, Park, Simmons, Pfeifer and Bou Ghanem. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging
Bhalla, Manmeet
Nayerhoda, Roozbeh
Tchalla, Essi Y. I.
Abamonte, Alexsandra
Park, Dongwon
Simmons, Shaunna R.
Pfeifer, Blaine A.
Bou Ghanem, Elsa N.
Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title_full Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title_fullStr Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title_full_unstemmed Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title_short Liposomal Encapsulation of Polysaccharides (LEPS) as an Effective Vaccine Strategy to Protect Aged Hosts Against S. pneumoniae Infection
title_sort liposomal encapsulation of polysaccharides (leps) as an effective vaccine strategy to protect aged hosts against s. pneumoniae infection
topic Aging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920316/
https://www.ncbi.nlm.nih.gov/pubmed/35291600
http://dx.doi.org/10.3389/fragi.2021.798868
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