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A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients

Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quan...

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Autores principales: Yu, Guangxin, Chen, Ran, Zheng, Sujun, Liu, Yanna, Zou, Jun, Gu, Zhiqiang, Jiang, Bei, Gao, Qi, Dai, Lizhong, Peng, Jie, Wang, Jie, Lu, Fengmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920369/
https://www.ncbi.nlm.nih.gov/pubmed/35220917
http://dx.doi.org/10.1080/22221751.2022.2045874
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author Yu, Guangxin
Chen, Ran
Zheng, Sujun
Liu, Yanna
Zou, Jun
Gu, Zhiqiang
Jiang, Bei
Gao, Qi
Dai, Lizhong
Peng, Jie
Wang, Jie
Lu, Fengmin
author_facet Yu, Guangxin
Chen, Ran
Zheng, Sujun
Liu, Yanna
Zou, Jun
Gu, Zhiqiang
Jiang, Bei
Gao, Qi
Dai, Lizhong
Peng, Jie
Wang, Jie
Lu, Fengmin
author_sort Yu, Guangxin
collection PubMed
description Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quantitative polymerase chain reactions for detecting the preC/C-RNA (preC/C region HBV pgRNA), SF-RNA (splicing variants-free pgRNA) and XR-RNA (X region remained pgRNA) regions were set up. The dynamic changes of serum pgRNA splicing variants and 3′ terminal truncations were analysed in three retrospective cohorts: 35 treatment-naive chronic HBV-infected patients (cohort A), 52 chronic hepatitis B (CHB) patients who received nucleos(t)ide analogs (NAs) therapy for 48 weeks (cohort B) and eight CHB patients who are under long-term NAs treatment (cohort C). The accuracy and sensitivity of HBV RNA detection were assessed by the National Standard of HBV RNA. We confirmed that high proportions of pgRNA splicing variants and 3′ terminal truncations were present and significantly affect the quantitative detection of serum HBV RNA in both treatment-naive and NAs-treated CHB patients. To achieve the higher accuracy and sensitivity on the detection of HBV RNA level, the primers and probes should be designed at the 5′ terminal region of HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs treatment. This study would help to better understand the significance of the pgRNA splicing variants and 3′ terminal truncations, and further guide the clinical detection of serum HBV RNA.
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spelling pubmed-89203692022-03-15 A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients Yu, Guangxin Chen, Ran Zheng, Sujun Liu, Yanna Zou, Jun Gu, Zhiqiang Jiang, Bei Gao, Qi Dai, Lizhong Peng, Jie Wang, Jie Lu, Fengmin Emerg Microbes Infect Hepatitis Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quantitative polymerase chain reactions for detecting the preC/C-RNA (preC/C region HBV pgRNA), SF-RNA (splicing variants-free pgRNA) and XR-RNA (X region remained pgRNA) regions were set up. The dynamic changes of serum pgRNA splicing variants and 3′ terminal truncations were analysed in three retrospective cohorts: 35 treatment-naive chronic HBV-infected patients (cohort A), 52 chronic hepatitis B (CHB) patients who received nucleos(t)ide analogs (NAs) therapy for 48 weeks (cohort B) and eight CHB patients who are under long-term NAs treatment (cohort C). The accuracy and sensitivity of HBV RNA detection were assessed by the National Standard of HBV RNA. We confirmed that high proportions of pgRNA splicing variants and 3′ terminal truncations were present and significantly affect the quantitative detection of serum HBV RNA in both treatment-naive and NAs-treated CHB patients. To achieve the higher accuracy and sensitivity on the detection of HBV RNA level, the primers and probes should be designed at the 5′ terminal region of HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs treatment. This study would help to better understand the significance of the pgRNA splicing variants and 3′ terminal truncations, and further guide the clinical detection of serum HBV RNA. Taylor & Francis 2022-03-10 /pmc/articles/PMC8920369/ /pubmed/35220917 http://dx.doi.org/10.1080/22221751.2022.2045874 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Hepatitis
Yu, Guangxin
Chen, Ran
Zheng, Sujun
Liu, Yanna
Zou, Jun
Gu, Zhiqiang
Jiang, Bei
Gao, Qi
Dai, Lizhong
Peng, Jie
Wang, Jie
Lu, Fengmin
A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title_full A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title_fullStr A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title_full_unstemmed A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title_short A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
title_sort standardized assay for the quantitative detection of serum hbv rna in chronic hepatitis b patients
topic Hepatitis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920369/
https://www.ncbi.nlm.nih.gov/pubmed/35220917
http://dx.doi.org/10.1080/22221751.2022.2045874
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