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A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients
Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quan...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920369/ https://www.ncbi.nlm.nih.gov/pubmed/35220917 http://dx.doi.org/10.1080/22221751.2022.2045874 |
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author | Yu, Guangxin Chen, Ran Zheng, Sujun Liu, Yanna Zou, Jun Gu, Zhiqiang Jiang, Bei Gao, Qi Dai, Lizhong Peng, Jie Wang, Jie Lu, Fengmin |
author_facet | Yu, Guangxin Chen, Ran Zheng, Sujun Liu, Yanna Zou, Jun Gu, Zhiqiang Jiang, Bei Gao, Qi Dai, Lizhong Peng, Jie Wang, Jie Lu, Fengmin |
author_sort | Yu, Guangxin |
collection | PubMed |
description | Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quantitative polymerase chain reactions for detecting the preC/C-RNA (preC/C region HBV pgRNA), SF-RNA (splicing variants-free pgRNA) and XR-RNA (X region remained pgRNA) regions were set up. The dynamic changes of serum pgRNA splicing variants and 3′ terminal truncations were analysed in three retrospective cohorts: 35 treatment-naive chronic HBV-infected patients (cohort A), 52 chronic hepatitis B (CHB) patients who received nucleos(t)ide analogs (NAs) therapy for 48 weeks (cohort B) and eight CHB patients who are under long-term NAs treatment (cohort C). The accuracy and sensitivity of HBV RNA detection were assessed by the National Standard of HBV RNA. We confirmed that high proportions of pgRNA splicing variants and 3′ terminal truncations were present and significantly affect the quantitative detection of serum HBV RNA in both treatment-naive and NAs-treated CHB patients. To achieve the higher accuracy and sensitivity on the detection of HBV RNA level, the primers and probes should be designed at the 5′ terminal region of HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs treatment. This study would help to better understand the significance of the pgRNA splicing variants and 3′ terminal truncations, and further guide the clinical detection of serum HBV RNA. |
format | Online Article Text |
id | pubmed-8920369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-89203692022-03-15 A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients Yu, Guangxin Chen, Ran Zheng, Sujun Liu, Yanna Zou, Jun Gu, Zhiqiang Jiang, Bei Gao, Qi Dai, Lizhong Peng, Jie Wang, Jie Lu, Fengmin Emerg Microbes Infect Hepatitis Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quantitative polymerase chain reactions for detecting the preC/C-RNA (preC/C region HBV pgRNA), SF-RNA (splicing variants-free pgRNA) and XR-RNA (X region remained pgRNA) regions were set up. The dynamic changes of serum pgRNA splicing variants and 3′ terminal truncations were analysed in three retrospective cohorts: 35 treatment-naive chronic HBV-infected patients (cohort A), 52 chronic hepatitis B (CHB) patients who received nucleos(t)ide analogs (NAs) therapy for 48 weeks (cohort B) and eight CHB patients who are under long-term NAs treatment (cohort C). The accuracy and sensitivity of HBV RNA detection were assessed by the National Standard of HBV RNA. We confirmed that high proportions of pgRNA splicing variants and 3′ terminal truncations were present and significantly affect the quantitative detection of serum HBV RNA in both treatment-naive and NAs-treated CHB patients. To achieve the higher accuracy and sensitivity on the detection of HBV RNA level, the primers and probes should be designed at the 5′ terminal region of HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs treatment. This study would help to better understand the significance of the pgRNA splicing variants and 3′ terminal truncations, and further guide the clinical detection of serum HBV RNA. Taylor & Francis 2022-03-10 /pmc/articles/PMC8920369/ /pubmed/35220917 http://dx.doi.org/10.1080/22221751.2022.2045874 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Hepatitis Yu, Guangxin Chen, Ran Zheng, Sujun Liu, Yanna Zou, Jun Gu, Zhiqiang Jiang, Bei Gao, Qi Dai, Lizhong Peng, Jie Wang, Jie Lu, Fengmin A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title | A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title_full | A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title_fullStr | A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title_full_unstemmed | A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title_short | A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients |
title_sort | standardized assay for the quantitative detection of serum hbv rna in chronic hepatitis b patients |
topic | Hepatitis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920369/ https://www.ncbi.nlm.nih.gov/pubmed/35220917 http://dx.doi.org/10.1080/22221751.2022.2045874 |
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