An optimized co-immunoprecipitation protocol for the analysis of endogenous protein-protein interactions in cell lines using mass spectrometry

This protocol represents an optimized proteomics-based protocol for the endogenous protein enrichment and protein-protein interaction analysis. This 2-step protocol consists of: 1) co-immunoprecipitation of the bait protein; 2) the bait-protein interactions analysis using LC-MS/MS. Here, we used Dyn...

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Detalles Bibliográficos
Autores principales: Lagundžin, Dragana, Krieger, Kimiko L., Law, Henry C.-H., Woods, Nicholas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920916/
https://www.ncbi.nlm.nih.gov/pubmed/35300004
http://dx.doi.org/10.1016/j.xpro.2022.101234

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