Cargando…

Generation of highly pure motor neurons from human induced pluripotent stem cells

Generation of human motor neurons (MNs) overcomes the inaccessibility to patient brain tissues and greatly facilitates the research in MN-related diseases. Here, we describe a protocol for generation of neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs), followed by pr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Akter, Masuma, Cui, Haochen, Sepehrimanesh, Masood, Hosain, Md Abir, Ding, Baojin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920922/ https://www.ncbi.nlm.nih.gov/pubmed/35300000 http://dx.doi.org/10.1016/j.xpro.2022.101223

Ejemplares similares

Protocol to image and quantify nucleocytoplasmic transport in cultured cells using fluorescent in situ hybridization and a dual reporter system
por: Cui, Haochen, et al.
Publicado: (2022)

Direct conversion of adult fibroblasts into motor neurons
por: Sepehrimanesh, Masood, et al.
Publicado: (2021)

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation P525L (c.1574C > T) in FUS gene
por: Akter, Masuma, et al.
Publicado: (2023)

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene
por: Akter, Masuma, et al.
Publicado: (2023)

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line
por: Akter, Masuma, et al.
Publicado: (2021)

Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
por: Akter, Masuma, et al.
Publicado: (2022)

Generation of gene-corrected isogenic control cell lines from a DYT1 dystonia patient iPSC line carrying a heterozygous GAG mutation in TOR1A gene
por: Akter, Masuma, et al.
Publicado: (2022)

Generation and Expansion of highly-pure Motor Neuron Progenitors from Human Pluripotent Stem Cells
por: Du, Zhong-Wei, et al.
Publicado: (2015)

Reproducible and scalable differentiation of highly pure cortical neurons from human induced pluripotent stem cells
por: Dannert, Angelika, et al.
Publicado: (2023)

Differential Influence of Sample Sex and Neuronal Maturation on mRNA and Protein Transport in Induced Human Neurons
por: Ding, Baojin, et al.
Publicado: (2020)

Nucleocytoplasmic Transport: Regulatory Mechanisms and the Implications in Neurodegeneration
por: Ding, Baojin, et al.
Publicado: (2021)

Generation of patient-specific motor neurons in modeling movement diseases
por: Ding, Baojin
Publicado: (2021)

High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
por: Sarrafha, Lily, et al.
Publicado: (2021)

Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation
por: Wali, Gautam, et al.
Publicado: (2023)

Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
por: Sheta, Razan, et al.
Publicado: (2023)

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
por: Knittel, Jacob, et al.
Publicado: (2022)

Generation and characterization of motor neuron progenitors and motor neurons using metachromatic leukodystrophy-induced pluripotent stem cells
por: Hossain, Mohammad Arif, et al.
Publicado: (2022)

Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
por: Mucci, Sofía, et al.
Publicado: (2022)

Generating Diverse Spinal Motor Neuron Subtypes from Human Pluripotent Stem Cells
por: Patani, Rickie
Publicado: (2016)

Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin
por: Kim, Hyosung, et al.
Publicado: (2021)

Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
por: Nafria, Monica, et al.
Publicado: (2020)

Protocol to generate cardiac pericytes from human induced pluripotent stem cells
por: Shen, Mengcheng, et al.
Publicado: (2023)

Generation of glutamatergic/GABAergic neuronal co-cultures derived from human induced pluripotent stem cells for characterizing E/I balance in vitro
por: Wang, Shan, et al.
Publicado: (2023)

A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells
por: Cutarelli, Alessandro, et al.
Publicado: (2021)

Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
por: Li, Ronghui, et al.
Publicado: (2021)

Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
por: Yin, Xiling, et al.
Publicado: (2021)

Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells
por: Chen, Luoman, et al.
Publicado: (2020)

Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells
por: Cao, Xu, et al.
Publicado: (2020)

An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
por: Haque, Mohammad, et al.
Publicado: (2021)

Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization
por: Anastasaki, Corina, et al.
Publicado: (2022)

An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids
por: Castellanos-Montiel, María José, et al.
Publicado: (2023)

Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays
por: Tan, Lay Shuen, et al.
Publicado: (2021)

Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells
por: Xi, Haibin, et al.
Publicado: (2020)

Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
por: Santos, Renata, et al.
Publicado: (2022)

Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions
por: Perriot, Sylvain, et al.
Publicado: (2022)

Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
por: Sharma, Ruchi, et al.
Publicado: (2022)

HIPRO: A High-Efficiency, Hypoxia-Induced Protocol for Generation of Photoreceptors in Retinal Organoids from Mouse Pluripotent Stem Cells
por: Chen, Holly Y., et al.
Publicado: (2020)

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System
por: Liu, Li-Ping, et al.
Publicado: (2020)

An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
por: Surendran, Harshini, et al.
Publicado: (2022)

Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
por: Pollock, Samuel D., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_dtra6i546b8ddidbcjpt10ussc