Mouse tail skin wholemount staining and intravital calcium imaging

Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicle...

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Detalles Bibliográficos
Autores principales: Xie, Yuhua, Chen, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920931/
https://www.ncbi.nlm.nih.gov/pubmed/35300002
http://dx.doi.org/10.1016/j.xpro.2022.101235
Descripción
Sumario:Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicles and stem cells in different physiological and pathological conditions. The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022).

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