Mouse tail skin wholemount staining and intravital calcium imaging

Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicle...

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Detalles Bibliográficos
Autores principales: Xie, Yuhua, Chen, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920931/
https://www.ncbi.nlm.nih.gov/pubmed/35300002
http://dx.doi.org/10.1016/j.xpro.2022.101235
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author Xie, Yuhua
Chen, Ting
author_facet Xie, Yuhua
Chen, Ting
author_sort Xie, Yuhua
collection PubMed
description Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicles and stem cells in different physiological and pathological conditions. The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022).
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spelling pubmed-89209312022-03-16 Mouse tail skin wholemount staining and intravital calcium imaging Xie, Yuhua Chen, Ting STAR Protoc Protocol Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicles and stem cells in different physiological and pathological conditions. The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022). Elsevier 2022-03-10 /pmc/articles/PMC8920931/ /pubmed/35300002 http://dx.doi.org/10.1016/j.xpro.2022.101235 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xie, Yuhua
Chen, Ting
Mouse tail skin wholemount staining and intravital calcium imaging
title Mouse tail skin wholemount staining and intravital calcium imaging
title_full Mouse tail skin wholemount staining and intravital calcium imaging
title_fullStr Mouse tail skin wholemount staining and intravital calcium imaging
title_full_unstemmed Mouse tail skin wholemount staining and intravital calcium imaging
title_short Mouse tail skin wholemount staining and intravital calcium imaging
title_sort mouse tail skin wholemount staining and intravital calcium imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920931/
https://www.ncbi.nlm.nih.gov/pubmed/35300002
http://dx.doi.org/10.1016/j.xpro.2022.101235
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