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STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway

Renal cell carcinoma is particularly sensitive to ferroptosis, an iron-dependent non-apoptotic form of cell death. This mechanism does not require activation of caspase or the participation of other apoptotic effector molecules (such as BAX or BAK), nor is it accompanied by the morphological charact...

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Autores principales: Ye, Cheng Lin, Du, Yang, Yu, Xi, Chen, Zhi Yuan, Wang, Lei, Zheng, Yong Fa, Liu, Xiu Heng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921746/
https://www.ncbi.nlm.nih.gov/pubmed/35275508
http://dx.doi.org/10.1177/15330338221078728
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author Ye, Cheng Lin
Du, Yang
Yu, Xi
Chen, Zhi Yuan
Wang, Lei
Zheng, Yong Fa
Liu, Xiu Heng
author_facet Ye, Cheng Lin
Du, Yang
Yu, Xi
Chen, Zhi Yuan
Wang, Lei
Zheng, Yong Fa
Liu, Xiu Heng
author_sort Ye, Cheng Lin
collection PubMed
description Renal cell carcinoma is particularly sensitive to ferroptosis, an iron-dependent non-apoptotic form of cell death. This mechanism does not require activation of caspase or the participation of other apoptotic effector molecules (such as BAX or BAK), nor is it accompanied by the morphological characteristics or biochemical processes of apoptosis. The STEAP3 gene was found because it promotes tumor apoptosis in prostate cancer, but its role in renal cell carcinoma has not been studied in depth. Through real-time quantitative polymerase chain reaction, we found that the expression of the STEAP3 gene was upregulated in renal cell carcinoma tissue samples and cell lines, and it was found to be highly expressed in renal cell carcinoma tissue through immunohistochemistry. This upregulation is related to poor survival and prognosis of patients. We used erastin, a ferroptosis inducer, found that renal cell carcinoma became more susceptible to ferroptosis after knocking down STEAP3. The results indicate that renal cell carcinoma cell lines with knocked down STEAP3 expression are more sensitive to ferroptosis, and this effect occurs through the p53/xCT pathway. In summary, our research helps to identify new biomarkers and provides new targets for the treatment of renal cell carcinoma.
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spelling pubmed-89217462022-03-16 STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway Ye, Cheng Lin Du, Yang Yu, Xi Chen, Zhi Yuan Wang, Lei Zheng, Yong Fa Liu, Xiu Heng Technol Cancer Res Treat Original Article Renal cell carcinoma is particularly sensitive to ferroptosis, an iron-dependent non-apoptotic form of cell death. This mechanism does not require activation of caspase or the participation of other apoptotic effector molecules (such as BAX or BAK), nor is it accompanied by the morphological characteristics or biochemical processes of apoptosis. The STEAP3 gene was found because it promotes tumor apoptosis in prostate cancer, but its role in renal cell carcinoma has not been studied in depth. Through real-time quantitative polymerase chain reaction, we found that the expression of the STEAP3 gene was upregulated in renal cell carcinoma tissue samples and cell lines, and it was found to be highly expressed in renal cell carcinoma tissue through immunohistochemistry. This upregulation is related to poor survival and prognosis of patients. We used erastin, a ferroptosis inducer, found that renal cell carcinoma became more susceptible to ferroptosis after knocking down STEAP3. The results indicate that renal cell carcinoma cell lines with knocked down STEAP3 expression are more sensitive to ferroptosis, and this effect occurs through the p53/xCT pathway. In summary, our research helps to identify new biomarkers and provides new targets for the treatment of renal cell carcinoma. SAGE Publications 2022-03-11 /pmc/articles/PMC8921746/ /pubmed/35275508 http://dx.doi.org/10.1177/15330338221078728 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Ye, Cheng Lin
Du, Yang
Yu, Xi
Chen, Zhi Yuan
Wang, Lei
Zheng, Yong Fa
Liu, Xiu Heng
STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title_full STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title_fullStr STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title_full_unstemmed STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title_short STEAP3 Affects Ferroptosis and Progression of Renal Cell Carcinoma Through the p53/xCT Pathway
title_sort steap3 affects ferroptosis and progression of renal cell carcinoma through the p53/xct pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921746/
https://www.ncbi.nlm.nih.gov/pubmed/35275508
http://dx.doi.org/10.1177/15330338221078728
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