Cargando…
The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921926/ https://www.ncbi.nlm.nih.gov/pubmed/35300481 http://dx.doi.org/10.3389/fmicb.2022.842721 |
_version_ | 1784669419139497984 |
---|---|
author | Zhai, Boyu Liu, Lanlan Li, Xiang Lv, Xinru Wu, Jinyan Li, Jing Lin, Shengze Yin, Yuxiang Lan, Jiaqi Du, Jianan Wu, Chenwei Wen, Yi Wang, Yajun Wang, Yulong Hou, Zhijun Li, Yanbing Chai, Hongliang Zeng, Xiangwei |
author_facet | Zhai, Boyu Liu, Lanlan Li, Xiang Lv, Xinru Wu, Jinyan Li, Jing Lin, Shengze Yin, Yuxiang Lan, Jiaqi Du, Jianan Wu, Chenwei Wen, Yi Wang, Yajun Wang, Yulong Hou, Zhijun Li, Yanbing Chai, Hongliang Zeng, Xiangwei |
author_sort | Zhai, Boyu |
collection | PubMed |
description | In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N8 virus, but little is known concerning innate immune responses during influenza infection in duck species. In this study, we used two wild-bird-origin viruses, H5N8 and H4N6, to conduct duck infection experiments, and detect the load of the two viruses, and retinoic acid-inducible gene I (RIG-I) and interferon β (IFN-β) in the host’s natural immune response. Through comparison, it is found that the expression levels of RIG-I and IFN-β are both fluctuating. The innate immunity starts rapidly within 6 h after infection and is inhibited by the virus to varying degrees. The expression of RIG-I and IFN-β decreased on 1–2 days post-infection (dpi). The HPAI H5N8 virus has a stronger inhibitory effect on RIG-I than the low pathogenic avian influenza (LPAI) H4N6 virus and is the strongest in the lungs. After infection with HPAI H5N8 virus, 2 dpi, viral RNA replicates in large amounts in the lungs. It has been proven that RIG-I and IFN-β play an important role in the innate immune response of ducks to HPAI H5N8 virus infection, especially in the lungs. The main battlefield of RIG-I and IFN-β after infection with the LPAI H4N6 virus is in the rectum. Both viruses have been effectively controlled after 7 dpi. These results will help to understand the transmission mechanisms of avian influenza virus in wild ducks and help effectively prevent and control avian influenza. |
format | Online Article Text |
id | pubmed-8921926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89219262022-03-16 The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses Zhai, Boyu Liu, Lanlan Li, Xiang Lv, Xinru Wu, Jinyan Li, Jing Lin, Shengze Yin, Yuxiang Lan, Jiaqi Du, Jianan Wu, Chenwei Wen, Yi Wang, Yajun Wang, Yulong Hou, Zhijun Li, Yanbing Chai, Hongliang Zeng, Xiangwei Front Microbiol Microbiology In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N8 virus, but little is known concerning innate immune responses during influenza infection in duck species. In this study, we used two wild-bird-origin viruses, H5N8 and H4N6, to conduct duck infection experiments, and detect the load of the two viruses, and retinoic acid-inducible gene I (RIG-I) and interferon β (IFN-β) in the host’s natural immune response. Through comparison, it is found that the expression levels of RIG-I and IFN-β are both fluctuating. The innate immunity starts rapidly within 6 h after infection and is inhibited by the virus to varying degrees. The expression of RIG-I and IFN-β decreased on 1–2 days post-infection (dpi). The HPAI H5N8 virus has a stronger inhibitory effect on RIG-I than the low pathogenic avian influenza (LPAI) H4N6 virus and is the strongest in the lungs. After infection with HPAI H5N8 virus, 2 dpi, viral RNA replicates in large amounts in the lungs. It has been proven that RIG-I and IFN-β play an important role in the innate immune response of ducks to HPAI H5N8 virus infection, especially in the lungs. The main battlefield of RIG-I and IFN-β after infection with the LPAI H4N6 virus is in the rectum. Both viruses have been effectively controlled after 7 dpi. These results will help to understand the transmission mechanisms of avian influenza virus in wild ducks and help effectively prevent and control avian influenza. Frontiers Media S.A. 2022-03-01 /pmc/articles/PMC8921926/ /pubmed/35300481 http://dx.doi.org/10.3389/fmicb.2022.842721 Text en Copyright © 2022 Zhai, Liu, Li, Lv, Wu, Li, Lin, Yin, Lan, Du, Wu, Wen, Wang, Wang, Hou, Li, Chai and Zeng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhai, Boyu Liu, Lanlan Li, Xiang Lv, Xinru Wu, Jinyan Li, Jing Lin, Shengze Yin, Yuxiang Lan, Jiaqi Du, Jianan Wu, Chenwei Wen, Yi Wang, Yajun Wang, Yulong Hou, Zhijun Li, Yanbing Chai, Hongliang Zeng, Xiangwei The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title | The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title_full | The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title_fullStr | The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title_full_unstemmed | The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title_short | The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses |
title_sort | variation of duck rig-i-mediated innate immune response induced by different virulence avian influenza viruses |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921926/ https://www.ncbi.nlm.nih.gov/pubmed/35300481 http://dx.doi.org/10.3389/fmicb.2022.842721 |
work_keys_str_mv | AT zhaiboyu thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT liulanlan thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lixiang thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lvxinru thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wujinyan thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lijing thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT linshengze thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT yinyuxiang thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lanjiaqi thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT dujianan thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wuchenwei thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wenyi thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wangyajun thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wangyulong thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT houzhijun thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT liyanbing thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT chaihongliang thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT zengxiangwei thevariationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT zhaiboyu variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT liulanlan variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lixiang variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lvxinru variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wujinyan variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lijing variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT linshengze variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT yinyuxiang variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT lanjiaqi variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT dujianan variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wuchenwei variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wenyi variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wangyajun variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT wangyulong variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT houzhijun variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT liyanbing variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT chaihongliang variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses AT zengxiangwei variationofduckrigimediatedinnateimmuneresponseinducedbydifferentvirulenceavianinfluenzaviruses |