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The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses

In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N...

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Autores principales: Zhai, Boyu, Liu, Lanlan, Li, Xiang, Lv, Xinru, Wu, Jinyan, Li, Jing, Lin, Shengze, Yin, Yuxiang, Lan, Jiaqi, Du, Jianan, Wu, Chenwei, Wen, Yi, Wang, Yajun, Wang, Yulong, Hou, Zhijun, Li, Yanbing, Chai, Hongliang, Zeng, Xiangwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921926/
https://www.ncbi.nlm.nih.gov/pubmed/35300481
http://dx.doi.org/10.3389/fmicb.2022.842721
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author Zhai, Boyu
Liu, Lanlan
Li, Xiang
Lv, Xinru
Wu, Jinyan
Li, Jing
Lin, Shengze
Yin, Yuxiang
Lan, Jiaqi
Du, Jianan
Wu, Chenwei
Wen, Yi
Wang, Yajun
Wang, Yulong
Hou, Zhijun
Li, Yanbing
Chai, Hongliang
Zeng, Xiangwei
author_facet Zhai, Boyu
Liu, Lanlan
Li, Xiang
Lv, Xinru
Wu, Jinyan
Li, Jing
Lin, Shengze
Yin, Yuxiang
Lan, Jiaqi
Du, Jianan
Wu, Chenwei
Wen, Yi
Wang, Yajun
Wang, Yulong
Hou, Zhijun
Li, Yanbing
Chai, Hongliang
Zeng, Xiangwei
author_sort Zhai, Boyu
collection PubMed
description In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N8 virus, but little is known concerning innate immune responses during influenza infection in duck species. In this study, we used two wild-bird-origin viruses, H5N8 and H4N6, to conduct duck infection experiments, and detect the load of the two viruses, and retinoic acid-inducible gene I (RIG-I) and interferon β (IFN-β) in the host’s natural immune response. Through comparison, it is found that the expression levels of RIG-I and IFN-β are both fluctuating. The innate immunity starts rapidly within 6 h after infection and is inhibited by the virus to varying degrees. The expression of RIG-I and IFN-β decreased on 1–2 days post-infection (dpi). The HPAI H5N8 virus has a stronger inhibitory effect on RIG-I than the low pathogenic avian influenza (LPAI) H4N6 virus and is the strongest in the lungs. After infection with HPAI H5N8 virus, 2 dpi, viral RNA replicates in large amounts in the lungs. It has been proven that RIG-I and IFN-β play an important role in the innate immune response of ducks to HPAI H5N8 virus infection, especially in the lungs. The main battlefield of RIG-I and IFN-β after infection with the LPAI H4N6 virus is in the rectum. Both viruses have been effectively controlled after 7 dpi. These results will help to understand the transmission mechanisms of avian influenza virus in wild ducks and help effectively prevent and control avian influenza.
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spelling pubmed-89219262022-03-16 The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses Zhai, Boyu Liu, Lanlan Li, Xiang Lv, Xinru Wu, Jinyan Li, Jing Lin, Shengze Yin, Yuxiang Lan, Jiaqi Du, Jianan Wu, Chenwei Wen, Yi Wang, Yajun Wang, Yulong Hou, Zhijun Li, Yanbing Chai, Hongliang Zeng, Xiangwei Front Microbiol Microbiology In recent years, the emerging highly pathogenic avian influenza (HPAI) A(H5N8) virus has been reported with features of widely spread, an expanding host range, and cross-species transmission, attracting wide attention. The domestic duck plays a major role in the epidemiological cycle of the HPAI H5N8 virus, but little is known concerning innate immune responses during influenza infection in duck species. In this study, we used two wild-bird-origin viruses, H5N8 and H4N6, to conduct duck infection experiments, and detect the load of the two viruses, and retinoic acid-inducible gene I (RIG-I) and interferon β (IFN-β) in the host’s natural immune response. Through comparison, it is found that the expression levels of RIG-I and IFN-β are both fluctuating. The innate immunity starts rapidly within 6 h after infection and is inhibited by the virus to varying degrees. The expression of RIG-I and IFN-β decreased on 1–2 days post-infection (dpi). The HPAI H5N8 virus has a stronger inhibitory effect on RIG-I than the low pathogenic avian influenza (LPAI) H4N6 virus and is the strongest in the lungs. After infection with HPAI H5N8 virus, 2 dpi, viral RNA replicates in large amounts in the lungs. It has been proven that RIG-I and IFN-β play an important role in the innate immune response of ducks to HPAI H5N8 virus infection, especially in the lungs. The main battlefield of RIG-I and IFN-β after infection with the LPAI H4N6 virus is in the rectum. Both viruses have been effectively controlled after 7 dpi. These results will help to understand the transmission mechanisms of avian influenza virus in wild ducks and help effectively prevent and control avian influenza. Frontiers Media S.A. 2022-03-01 /pmc/articles/PMC8921926/ /pubmed/35300481 http://dx.doi.org/10.3389/fmicb.2022.842721 Text en Copyright © 2022 Zhai, Liu, Li, Lv, Wu, Li, Lin, Yin, Lan, Du, Wu, Wen, Wang, Wang, Hou, Li, Chai and Zeng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhai, Boyu
Liu, Lanlan
Li, Xiang
Lv, Xinru
Wu, Jinyan
Li, Jing
Lin, Shengze
Yin, Yuxiang
Lan, Jiaqi
Du, Jianan
Wu, Chenwei
Wen, Yi
Wang, Yajun
Wang, Yulong
Hou, Zhijun
Li, Yanbing
Chai, Hongliang
Zeng, Xiangwei
The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title_full The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title_fullStr The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title_full_unstemmed The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title_short The Variation of Duck RIG-I-Mediated Innate Immune Response Induced by Different Virulence Avian Influenza Viruses
title_sort variation of duck rig-i-mediated innate immune response induced by different virulence avian influenza viruses
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921926/
https://www.ncbi.nlm.nih.gov/pubmed/35300481
http://dx.doi.org/10.3389/fmicb.2022.842721
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