A Role for Taok2 in Listeria monocytogenes Vacuolar Escape

The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture...

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Autores principales: Quereda, Juan J, Morel, Camille, Lopez-Montero, Noelia, Ziveri, Jason, Rolland, Steven, Grenier, Théodore, Aulner, Nathalie, Danckaert, Anne, Charbit, Alain, Enninga, Jost, Cossart, Pascale, Pizarro-Cerdá, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Major Articles and Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922001/
https://www.ncbi.nlm.nih.gov/pubmed/32582947
http://dx.doi.org/10.1093/infdis/jiaa367
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author Quereda, Juan J
Morel, Camille
Lopez-Montero, Noelia
Ziveri, Jason
Rolland, Steven
Grenier, Théodore
Aulner, Nathalie
Danckaert, Anne
Charbit, Alain
Enninga, Jost
Cossart, Pascale
Pizarro-Cerdá, Javier
author_facet Quereda, Juan J
Morel, Camille
Lopez-Montero, Noelia
Ziveri, Jason
Rolland, Steven
Grenier, Théodore
Aulner, Nathalie
Danckaert, Anne
Charbit, Alain
Enninga, Jost
Cossart, Pascale
Pizarro-Cerdá, Javier
author_sort Quereda, Juan J
collection PubMed
description The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
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spelling pubmed-89220012022-03-15 A Role for Taok2 in Listeria monocytogenes Vacuolar Escape Quereda, Juan J Morel, Camille Lopez-Montero, Noelia Ziveri, Jason Rolland, Steven Grenier, Théodore Aulner, Nathalie Danckaert, Anne Charbit, Alain Enninga, Jost Cossart, Pascale Pizarro-Cerdá, Javier J Infect Dis Major Articles and Brief Reports The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells. Oxford University Press 2020-06-25 /pmc/articles/PMC8922001/ /pubmed/32582947 http://dx.doi.org/10.1093/infdis/jiaa367 Text en © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Major Articles and Brief Reports
Quereda, Juan J
Morel, Camille
Lopez-Montero, Noelia
Ziveri, Jason
Rolland, Steven
Grenier, Théodore
Aulner, Nathalie
Danckaert, Anne
Charbit, Alain
Enninga, Jost
Cossart, Pascale
Pizarro-Cerdá, Javier
A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title_full A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title_fullStr A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title_full_unstemmed A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title_short A Role for Taok2 in Listeria monocytogenes Vacuolar Escape
title_sort role for taok2 in listeria monocytogenes vacuolar escape
topic Major Articles and Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922001/
https://www.ncbi.nlm.nih.gov/pubmed/32582947
http://dx.doi.org/10.1093/infdis/jiaa367
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