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Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea

Diarrhea is the third leading cause of death in developing countries in children under the age of five. About half a million children die of diarrhea every year, most of which in developing countries. Viruses are the main pathogen of diarrhea. In China, the fecal virome of children with diarrhea has...

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Autores principales: Yang, Shixing, He, Yumin, Zhang, Ju, Zhang, Dianqi, Wang, Yan, Lu, Xiang, Wang, Xiaochun, Shen, Quan, Ji, Likai, Lu, Hongyan, Zhang, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922427/
https://www.ncbi.nlm.nih.gov/pubmed/35234620
http://dx.doi.org/10.1016/j.virs.2022.01.012
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author Yang, Shixing
He, Yumin
Zhang, Ju
Zhang, Dianqi
Wang, Yan
Lu, Xiang
Wang, Xiaochun
Shen, Quan
Ji, Likai
Lu, Hongyan
Zhang, Wen
author_facet Yang, Shixing
He, Yumin
Zhang, Ju
Zhang, Dianqi
Wang, Yan
Lu, Xiang
Wang, Xiaochun
Shen, Quan
Ji, Likai
Lu, Hongyan
Zhang, Wen
author_sort Yang, Shixing
collection PubMed
description Diarrhea is the third leading cause of death in developing countries in children under the age of five. About half a million children die of diarrhea every year, most of which in developing countries. Viruses are the main pathogen of diarrhea. In China, the fecal virome of children with diarrhea has been rarely studied. Using an unbiased viral metagenomics approach, we analyzed the fecal virome in children with diarrhea. Many DNA or RNA viruses associated with diarrhea identified in those fecal samples were mainly from six families of Adenoviridae, Astroviridae, Caliciviridae, Parvoviridae, Picornaviridae, and Reoviridae. Among them, the family of Caliciviridae accounts for the largest proportion of 78.42%, following with Adenoviridae (8.94%) and Picornaviridae (8.36%). In addition to those diarrhea-related viruses that have already been confirmed to cause human diarrhea, the viruses not associated with diarrhea were also identified including anellovirus and picobirnavirus. This study increased our understanding of diarrheic children fecal virome and provided valuable information for the prevention and treatment of viral diarrhea in this area.
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spelling pubmed-89224272022-03-21 Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea Yang, Shixing He, Yumin Zhang, Ju Zhang, Dianqi Wang, Yan Lu, Xiang Wang, Xiaochun Shen, Quan Ji, Likai Lu, Hongyan Zhang, Wen Virol Sin Research Article Diarrhea is the third leading cause of death in developing countries in children under the age of five. About half a million children die of diarrhea every year, most of which in developing countries. Viruses are the main pathogen of diarrhea. In China, the fecal virome of children with diarrhea has been rarely studied. Using an unbiased viral metagenomics approach, we analyzed the fecal virome in children with diarrhea. Many DNA or RNA viruses associated with diarrhea identified in those fecal samples were mainly from six families of Adenoviridae, Astroviridae, Caliciviridae, Parvoviridae, Picornaviridae, and Reoviridae. Among them, the family of Caliciviridae accounts for the largest proportion of 78.42%, following with Adenoviridae (8.94%) and Picornaviridae (8.36%). In addition to those diarrhea-related viruses that have already been confirmed to cause human diarrhea, the viruses not associated with diarrhea were also identified including anellovirus and picobirnavirus. This study increased our understanding of diarrheic children fecal virome and provided valuable information for the prevention and treatment of viral diarrhea in this area. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-01-17 /pmc/articles/PMC8922427/ /pubmed/35234620 http://dx.doi.org/10.1016/j.virs.2022.01.012 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Yang, Shixing
He, Yumin
Zhang, Ju
Zhang, Dianqi
Wang, Yan
Lu, Xiang
Wang, Xiaochun
Shen, Quan
Ji, Likai
Lu, Hongyan
Zhang, Wen
Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title_full Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title_fullStr Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title_full_unstemmed Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title_short Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
title_sort viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922427/
https://www.ncbi.nlm.nih.gov/pubmed/35234620
http://dx.doi.org/10.1016/j.virs.2022.01.012
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