Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples

A quantitative, high throughput, fully automated diagnostic method for the detection of neutralizing anti-SARS-CoV-2 antibodies was developed on the Phadia system based on the interaction of SARS-CoV-2 S1 protein and the human ACE-2 receptor. This method was compared to the current state of the art...

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Autores principales: Filchtinski, Daniel, Sundberg, Magnus, Berthold, Heike, Steller, Laura, Kayser, José, Holz, Sanja, Hinze, Mario, Braeutigam, Oxana, Schulte-Pelkum, Johannes, Fiedler, Raimund
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thermo Fisher Scientific ImmunoDiagnostics Division Freiburg. Published by Elsevier B.V. 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923036/
https://www.ncbi.nlm.nih.gov/pubmed/35304119
http://dx.doi.org/10.1016/j.jim.2022.113258
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author Filchtinski, Daniel
Sundberg, Magnus
Berthold, Heike
Steller, Laura
Kayser, José
Holz, Sanja
Hinze, Mario
Braeutigam, Oxana
Schulte-Pelkum, Johannes
Fiedler, Raimund
author_facet Filchtinski, Daniel
Sundberg, Magnus
Berthold, Heike
Steller, Laura
Kayser, José
Holz, Sanja
Hinze, Mario
Braeutigam, Oxana
Schulte-Pelkum, Johannes
Fiedler, Raimund
author_sort Filchtinski, Daniel
collection PubMed
description A quantitative, high throughput, fully automated diagnostic method for the detection of neutralizing anti-SARS-CoV-2 antibodies was developed on the Phadia system based on the interaction of SARS-CoV-2 S1 protein and the human ACE-2 receptor. This method was compared to the current state of the art plaque reduction neutralization test (PRNT) and a high correlation between the two methods was observed. Using a large cohort of blood samples from convalescent patients and controls the method displays very high sensitivity and specificity (99,8% and 99.99%, respectively). Neutralizing antibody titers of mRNA-1273 and BNT162b2-vaccinated persons can also be quantified with this method as well. This fully automated method provides the possibility to determine anti-SARS-CoV-2 neutralizing antibody concentrations in just 2  h.
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spelling pubmed-89230362022-03-15 Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples Filchtinski, Daniel Sundberg, Magnus Berthold, Heike Steller, Laura Kayser, José Holz, Sanja Hinze, Mario Braeutigam, Oxana Schulte-Pelkum, Johannes Fiedler, Raimund J Immunol Methods Article A quantitative, high throughput, fully automated diagnostic method for the detection of neutralizing anti-SARS-CoV-2 antibodies was developed on the Phadia system based on the interaction of SARS-CoV-2 S1 protein and the human ACE-2 receptor. This method was compared to the current state of the art plaque reduction neutralization test (PRNT) and a high correlation between the two methods was observed. Using a large cohort of blood samples from convalescent patients and controls the method displays very high sensitivity and specificity (99,8% and 99.99%, respectively). Neutralizing antibody titers of mRNA-1273 and BNT162b2-vaccinated persons can also be quantified with this method as well. This fully automated method provides the possibility to determine anti-SARS-CoV-2 neutralizing antibody concentrations in just 2  h. Thermo Fisher Scientific ImmunoDiagnostics Division Freiburg. Published by Elsevier B.V. 2022-05 2022-03-15 /pmc/articles/PMC8923036/ /pubmed/35304119 http://dx.doi.org/10.1016/j.jim.2022.113258 Text en © 2022 Thermo Fisher Scientific ImmunoDiagnostics Division Freiburg Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Filchtinski, Daniel
Sundberg, Magnus
Berthold, Heike
Steller, Laura
Kayser, José
Holz, Sanja
Hinze, Mario
Braeutigam, Oxana
Schulte-Pelkum, Johannes
Fiedler, Raimund
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title_full Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title_fullStr Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title_full_unstemmed Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title_short Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples
title_sort application of the sars-cov-2-s1 ace-2 receptor interaction as the basis of the fully automated assay to detect neutralizing sars-cov-2-s1 antibodies in blood samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923036/
https://www.ncbi.nlm.nih.gov/pubmed/35304119
http://dx.doi.org/10.1016/j.jim.2022.113258
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