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Changes in gut microbiota composition with age and correlations with gut inflammation in rats
Increasing evidences indicate that gut microbiota composition is associated with multiple inflammatory diseases. However, little is known about how gut microbiota changes with age and correlations with gut inflammation at sexual maturity stage of healthy individuals. Elucidating the dynamic changes...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923432/ https://www.ncbi.nlm.nih.gov/pubmed/35290412 http://dx.doi.org/10.1371/journal.pone.0265430 |
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author | Meng, Chen Feng, Siyuan Hao, Zikai Dong, Chen Liu, Hong |
author_facet | Meng, Chen Feng, Siyuan Hao, Zikai Dong, Chen Liu, Hong |
author_sort | Meng, Chen |
collection | PubMed |
description | Increasing evidences indicate that gut microbiota composition is associated with multiple inflammatory diseases. However, little is known about how gut microbiota changes with age and correlations with gut inflammation at sexual maturity stage of healthy individuals. Elucidating the dynamic changes of gut microbiota in healthy individuals at the sexual maturity stage and correlations with gut inflammation can provide clues for early risk assessment of gut diseases at the sexual maturity stage. Here, the shift in gut bacteria and its relationship with gut inflammation at the sexual maturity stage were explored. Sprague–Dawley rats at the sexual maturity stage were used in this study. 16S rRNA gene sequencing was performed to decipher gut bacteria shifts from the 7th week to the 9th week, and enzyme-linked immunosorbent assay (ELISA) was used to measure gut inflammation and gut barrier permeability. We found an increase in bacterial richness with age and a decrease in bacterial diversity with age. The gut bacteria were primarily dominated by the phyla Firmicutes and Bacteroides and the genus Prevotella. The relative abundance of Firmicutes increased with age, and the relative abundance of Bacteroides decreased with age. There was a positive correlation between body weight and the Firmicutes:Bacteroides ratio. More and more gut microbiota participated in the host gut inflammation and barrier permeability regulation with age. Ruminococcus was the only gut bacteria participated in gut inflammation and barrier permeability regulation both in the 7th week and the 15th week. These results provide a better understanding of the relationship between gut bacteria and gut inflammation in sexually mature rats and show that Ruminococcus may be a potential indicator for early risk assessment of gut inflammation. |
format | Online Article Text |
id | pubmed-8923432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89234322022-03-16 Changes in gut microbiota composition with age and correlations with gut inflammation in rats Meng, Chen Feng, Siyuan Hao, Zikai Dong, Chen Liu, Hong PLoS One Research Article Increasing evidences indicate that gut microbiota composition is associated with multiple inflammatory diseases. However, little is known about how gut microbiota changes with age and correlations with gut inflammation at sexual maturity stage of healthy individuals. Elucidating the dynamic changes of gut microbiota in healthy individuals at the sexual maturity stage and correlations with gut inflammation can provide clues for early risk assessment of gut diseases at the sexual maturity stage. Here, the shift in gut bacteria and its relationship with gut inflammation at the sexual maturity stage were explored. Sprague–Dawley rats at the sexual maturity stage were used in this study. 16S rRNA gene sequencing was performed to decipher gut bacteria shifts from the 7th week to the 9th week, and enzyme-linked immunosorbent assay (ELISA) was used to measure gut inflammation and gut barrier permeability. We found an increase in bacterial richness with age and a decrease in bacterial diversity with age. The gut bacteria were primarily dominated by the phyla Firmicutes and Bacteroides and the genus Prevotella. The relative abundance of Firmicutes increased with age, and the relative abundance of Bacteroides decreased with age. There was a positive correlation between body weight and the Firmicutes:Bacteroides ratio. More and more gut microbiota participated in the host gut inflammation and barrier permeability regulation with age. Ruminococcus was the only gut bacteria participated in gut inflammation and barrier permeability regulation both in the 7th week and the 15th week. These results provide a better understanding of the relationship between gut bacteria and gut inflammation in sexually mature rats and show that Ruminococcus may be a potential indicator for early risk assessment of gut inflammation. Public Library of Science 2022-03-15 /pmc/articles/PMC8923432/ /pubmed/35290412 http://dx.doi.org/10.1371/journal.pone.0265430 Text en © 2022 Meng et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Meng, Chen Feng, Siyuan Hao, Zikai Dong, Chen Liu, Hong Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title | Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title_full | Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title_fullStr | Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title_full_unstemmed | Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title_short | Changes in gut microbiota composition with age and correlations with gut inflammation in rats |
title_sort | changes in gut microbiota composition with age and correlations with gut inflammation in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923432/ https://www.ncbi.nlm.nih.gov/pubmed/35290412 http://dx.doi.org/10.1371/journal.pone.0265430 |
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