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Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli

The present study involves isolation of Streptomyces spp. from rhizosphere of Coscinium fenestratum Gaertn, an endangered medicinal plant from Western Ghats of Karnataka, India. Four potential isolates were identified by 16S rRNA sequencing as Streptomyces sp. RHPR3, Streptomyces puniceus RHPR9, Str...

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Autores principales: Ravinder, Polapally, Manasa, M., Roopa, D., Bukhari, Najat A., Hatamleh, Ashraf Atef, Khan, Mohamed Yahya, M. S., Reddy, Hameeda, Bee, El Enshasy, Hesham Ali, Hanapi, Siti Zulaiha, Sayyed, R. Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923452/
https://www.ncbi.nlm.nih.gov/pubmed/35290374
http://dx.doi.org/10.1371/journal.pone.0264975
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author Ravinder, Polapally
Manasa, M.
Roopa, D.
Bukhari, Najat A.
Hatamleh, Ashraf Atef
Khan, Mohamed Yahya
M. S., Reddy
Hameeda, Bee
El Enshasy, Hesham Ali
Hanapi, Siti Zulaiha
Sayyed, R. Z.
author_facet Ravinder, Polapally
Manasa, M.
Roopa, D.
Bukhari, Najat A.
Hatamleh, Ashraf Atef
Khan, Mohamed Yahya
M. S., Reddy
Hameeda, Bee
El Enshasy, Hesham Ali
Hanapi, Siti Zulaiha
Sayyed, R. Z.
author_sort Ravinder, Polapally
collection PubMed
description The present study involves isolation of Streptomyces spp. from rhizosphere of Coscinium fenestratum Gaertn, an endangered medicinal plant from Western Ghats of Karnataka, India. Four potential isolates were identified by 16S rRNA sequencing as Streptomyces sp. RHPR3, Streptomyces puniceus RHPR9, Streptomyces sp. RHPR14 and Streptomyces mediolani RHPR25. An enrichment culture method was used for the isolation of Streptomyces spp. for biosurfactant activity. Among four potential Streptomyces spp., S. puniceus RHPR9 showed highest Emulsification index (EI) (78±0.2%) and Emulsification assay (EA) (223±0.2 EU mL(-1)). Thin layer chromatography, Fourier transform infrared spectroscopy (FTIR) and mass spectrometric analysis revealed that as glycolipid. Further confirmed by presence of fatty acids like hexanoic acid methyl ester, decanoic acid by Gas chromatography mass spectroscopy (GC-MS) analysis. S. puniceus RHPR9 showed a significant IAA production (41μg mL(-1)), solubilized P (749.1 μg mL(-1)), growth promotion of chilli (Capsicum annuum L.) was evaluated using paper towel method and greenhouse conditions. S. puniceus RHPR9 showed a significant increase in seed vigor index (2047) and increase in plant biomass (65%) when compared to uninoculated control. To our knowledge, this is the first report on epiphytic S. puniceus RHPR9 isolated from an endangered medicinal plant C. fenestratum Gaertn, for biosurfactant production and plant growth promotion activities.
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spelling pubmed-89234522022-03-16 Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli Ravinder, Polapally Manasa, M. Roopa, D. Bukhari, Najat A. Hatamleh, Ashraf Atef Khan, Mohamed Yahya M. S., Reddy Hameeda, Bee El Enshasy, Hesham Ali Hanapi, Siti Zulaiha Sayyed, R. Z. PLoS One Research Article The present study involves isolation of Streptomyces spp. from rhizosphere of Coscinium fenestratum Gaertn, an endangered medicinal plant from Western Ghats of Karnataka, India. Four potential isolates were identified by 16S rRNA sequencing as Streptomyces sp. RHPR3, Streptomyces puniceus RHPR9, Streptomyces sp. RHPR14 and Streptomyces mediolani RHPR25. An enrichment culture method was used for the isolation of Streptomyces spp. for biosurfactant activity. Among four potential Streptomyces spp., S. puniceus RHPR9 showed highest Emulsification index (EI) (78±0.2%) and Emulsification assay (EA) (223±0.2 EU mL(-1)). Thin layer chromatography, Fourier transform infrared spectroscopy (FTIR) and mass spectrometric analysis revealed that as glycolipid. Further confirmed by presence of fatty acids like hexanoic acid methyl ester, decanoic acid by Gas chromatography mass spectroscopy (GC-MS) analysis. S. puniceus RHPR9 showed a significant IAA production (41μg mL(-1)), solubilized P (749.1 μg mL(-1)), growth promotion of chilli (Capsicum annuum L.) was evaluated using paper towel method and greenhouse conditions. S. puniceus RHPR9 showed a significant increase in seed vigor index (2047) and increase in plant biomass (65%) when compared to uninoculated control. To our knowledge, this is the first report on epiphytic S. puniceus RHPR9 isolated from an endangered medicinal plant C. fenestratum Gaertn, for biosurfactant production and plant growth promotion activities. Public Library of Science 2022-03-15 /pmc/articles/PMC8923452/ /pubmed/35290374 http://dx.doi.org/10.1371/journal.pone.0264975 Text en © 2022 Ravinder et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ravinder, Polapally
Manasa, M.
Roopa, D.
Bukhari, Najat A.
Hatamleh, Ashraf Atef
Khan, Mohamed Yahya
M. S., Reddy
Hameeda, Bee
El Enshasy, Hesham Ali
Hanapi, Siti Zulaiha
Sayyed, R. Z.
Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title_full Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title_fullStr Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title_full_unstemmed Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title_short Biosurfactant producing multifarious Streptomyces puniceus RHPR9 of Coscinium fenestratum rhizosphere promotes plant growth in chilli
title_sort biosurfactant producing multifarious streptomyces puniceus rhpr9 of coscinium fenestratum rhizosphere promotes plant growth in chilli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923452/
https://www.ncbi.nlm.nih.gov/pubmed/35290374
http://dx.doi.org/10.1371/journal.pone.0264975
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