Cargando…

Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7

Copper oxide (CuO) nanoparticle- (NP-) decorated carbon NPs (CNPs) were produced as colloidal suspension through pulsed laser ablation technique in liquid (PLAL) medium. The antimicrobial activity of the produced NPs was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohammed, Salman A. A., Khashan, Khawla S., Jabir, Majid S., Abdulameer, Farah A., Sulaiman, Ghassan M., Al-Omar, Mohsen S., Mohammed, Hamdoon A., Hadi, Aseel A., Khan, Riaz A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923787/
https://www.ncbi.nlm.nih.gov/pubmed/35299896
http://dx.doi.org/10.1155/2022/9863616
_version_ 1784669735037698048
author Mohammed, Salman A. A.
Khashan, Khawla S.
Jabir, Majid S.
Abdulameer, Farah A.
Sulaiman, Ghassan M.
Al-Omar, Mohsen S.
Mohammed, Hamdoon A.
Hadi, Aseel A.
Khan, Riaz A.
author_facet Mohammed, Salman A. A.
Khashan, Khawla S.
Jabir, Majid S.
Abdulameer, Farah A.
Sulaiman, Ghassan M.
Al-Omar, Mohsen S.
Mohammed, Hamdoon A.
Hadi, Aseel A.
Khan, Riaz A.
author_sort Mohammed, Salman A. A.
collection PubMed
description Copper oxide (CuO) nanoparticle- (NP-) decorated carbon NPs (CNPs) were produced as colloidal suspension through pulsed laser ablation technique in liquid (PLAL) medium. The antimicrobial activity of the produced NPs was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and anticancer activity was tested against breast cancer cell line, MCF-7, together with the biocompatibility assessment of these NPs. The X-ray diffraction (XRD) patterns of the obtained CNPs showed peaks at 26.58° and 43.78° (2θ) identical to (002) and (111) planes, respectively, of the carbon phases. It also displayed new peaks at 38.5° and 48.64° (2θ) after doping with CuO NPs. Transmission electron microscope (TEM) images revealed the crystalline nature with the spherical shape of the prepared CNPs with 5-40 nm diameter ranges. In addition, the NP effects on the bacterial cell walls and nucleic acid were confirmed using a scanning electron microscope (SEM) and microscopic fluorescence analysis. The NPs showed antibacterial activity through SEM examinations against the pathogenic microbial species, S. aureus and E. coli. In the cellular material release assay, the optical density of the bacterial cells, treated with NPs, displayed a significant increase with the time of exposure to NPs, and the cytotoxicity reached more than 80% of the level for the CNPs decorated with CuO NPs. The morphology of the MCF-7 cells treated with NPs decreased numbers, and the loss of contact with the surrounding cells was observed. These results confirmed that the CNPs decorated with CuO NPs have no observable side effects and can be safely used for therapeutic applications. It is also noteworthy that it is the first report of preparation of CuO NPs decorated with CNPs (CuO NPs-CNPs) by PLAL, and the produced NPs showed antimicrobial antiproliferative activities against breast cancer cell lines, MCF-7. The main advantage of the PLAL technique of synthesizing CuO NPs-CNPs provided a two-step, cost-effective, and eco-friendly method.
format Online
Article
Text
id pubmed-8923787
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-89237872022-03-16 Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7 Mohammed, Salman A. A. Khashan, Khawla S. Jabir, Majid S. Abdulameer, Farah A. Sulaiman, Ghassan M. Al-Omar, Mohsen S. Mohammed, Hamdoon A. Hadi, Aseel A. Khan, Riaz A. Biomed Res Int Research Article Copper oxide (CuO) nanoparticle- (NP-) decorated carbon NPs (CNPs) were produced as colloidal suspension through pulsed laser ablation technique in liquid (PLAL) medium. The antimicrobial activity of the produced NPs was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and anticancer activity was tested against breast cancer cell line, MCF-7, together with the biocompatibility assessment of these NPs. The X-ray diffraction (XRD) patterns of the obtained CNPs showed peaks at 26.58° and 43.78° (2θ) identical to (002) and (111) planes, respectively, of the carbon phases. It also displayed new peaks at 38.5° and 48.64° (2θ) after doping with CuO NPs. Transmission electron microscope (TEM) images revealed the crystalline nature with the spherical shape of the prepared CNPs with 5-40 nm diameter ranges. In addition, the NP effects on the bacterial cell walls and nucleic acid were confirmed using a scanning electron microscope (SEM) and microscopic fluorescence analysis. The NPs showed antibacterial activity through SEM examinations against the pathogenic microbial species, S. aureus and E. coli. In the cellular material release assay, the optical density of the bacterial cells, treated with NPs, displayed a significant increase with the time of exposure to NPs, and the cytotoxicity reached more than 80% of the level for the CNPs decorated with CuO NPs. The morphology of the MCF-7 cells treated with NPs decreased numbers, and the loss of contact with the surrounding cells was observed. These results confirmed that the CNPs decorated with CuO NPs have no observable side effects and can be safely used for therapeutic applications. It is also noteworthy that it is the first report of preparation of CuO NPs decorated with CNPs (CuO NPs-CNPs) by PLAL, and the produced NPs showed antimicrobial antiproliferative activities against breast cancer cell lines, MCF-7. The main advantage of the PLAL technique of synthesizing CuO NPs-CNPs provided a two-step, cost-effective, and eco-friendly method. Hindawi 2022-03-08 /pmc/articles/PMC8923787/ /pubmed/35299896 http://dx.doi.org/10.1155/2022/9863616 Text en Copyright © 2022 Salman A. A. Mohammed et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mohammed, Salman A. A.
Khashan, Khawla S.
Jabir, Majid S.
Abdulameer, Farah A.
Sulaiman, Ghassan M.
Al-Omar, Mohsen S.
Mohammed, Hamdoon A.
Hadi, Aseel A.
Khan, Riaz A.
Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title_full Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title_fullStr Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title_full_unstemmed Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title_short Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7
title_sort copper oxide nanoparticle-decorated carbon nanoparticle composite colloidal preparation through laser ablation for antimicrobial and antiproliferative actions against breast cancer cell line, mcf-7
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923787/
https://www.ncbi.nlm.nih.gov/pubmed/35299896
http://dx.doi.org/10.1155/2022/9863616
work_keys_str_mv AT mohammedsalmanaa copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT khashankhawlas copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT jabirmajids copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT abdulameerfaraha copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT sulaimanghassanm copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT alomarmohsens copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT mohammedhamdoona copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT hadiaseela copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7
AT khanriaza copperoxidenanoparticledecoratedcarbonnanoparticlecompositecolloidalpreparationthroughlaserablationforantimicrobialandantiproliferativeactionsagainstbreastcancercelllinemcf7