Cargando…

Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS

Infection with Helicobacter pylori (H. pylori) affects almost half of the world’s population and is a major cause of stomach cancer. Although immune cells react strongly to this gastric bacterium, H. pylori is still one of the rare pathogens that can evade elimination by the host and cause chronic i...

Descripción completa

Detalles Bibliográficos
Autores principales: Frauenlob, Tobias, Neuper, Theresa, Mehinagic, Muamera, Dang, Hieu-Hoa, Boraschi, Diana, Horejs-Hoeck, Jutta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924073/
https://www.ncbi.nlm.nih.gov/pubmed/35309333
http://dx.doi.org/10.3389/fimmu.2022.847958
_version_ 1784669768029044736
author Frauenlob, Tobias
Neuper, Theresa
Mehinagic, Muamera
Dang, Hieu-Hoa
Boraschi, Diana
Horejs-Hoeck, Jutta
author_facet Frauenlob, Tobias
Neuper, Theresa
Mehinagic, Muamera
Dang, Hieu-Hoa
Boraschi, Diana
Horejs-Hoeck, Jutta
author_sort Frauenlob, Tobias
collection PubMed
description Infection with Helicobacter pylori (H. pylori) affects almost half of the world’s population and is a major cause of stomach cancer. Although immune cells react strongly to this gastric bacterium, H. pylori is still one of the rare pathogens that can evade elimination by the host and cause chronic inflammation. In the present study, we characterized the inflammatory response of primary human monocytes to repeated H. pylori infection and their responsiveness to an ensuing bacterial stimulus. We show that, although repeated stimulations with H. pylori do not result in an enhanced response, H. pylori-primed monocytes are hyper-responsive to an Escherichia coli-lipopolysaccharide (LPS) stimulation that takes place shortly after infection. This hyper-responsiveness to bacterial stimuli is observed upon infection with viable H. pylori only, while heat-killed H. pylori fails to boost both cytokine secretion and STAT activation in response to LPS. When the secondary challenge occurs several days after the primary infection with live bacteria, H. pylori-infected monocytes lose their hyper-responsiveness. The observation that H. pylori makes primary human monocytes more susceptible to subsequent/overlapping stimuli provides an important basis to better understand how H. pylori can maintain chronic inflammation and thus contribute to gastric cancer progression.
format Online
Article
Text
id pubmed-8924073
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89240732022-03-17 Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS Frauenlob, Tobias Neuper, Theresa Mehinagic, Muamera Dang, Hieu-Hoa Boraschi, Diana Horejs-Hoeck, Jutta Front Immunol Immunology Infection with Helicobacter pylori (H. pylori) affects almost half of the world’s population and is a major cause of stomach cancer. Although immune cells react strongly to this gastric bacterium, H. pylori is still one of the rare pathogens that can evade elimination by the host and cause chronic inflammation. In the present study, we characterized the inflammatory response of primary human monocytes to repeated H. pylori infection and their responsiveness to an ensuing bacterial stimulus. We show that, although repeated stimulations with H. pylori do not result in an enhanced response, H. pylori-primed monocytes are hyper-responsive to an Escherichia coli-lipopolysaccharide (LPS) stimulation that takes place shortly after infection. This hyper-responsiveness to bacterial stimuli is observed upon infection with viable H. pylori only, while heat-killed H. pylori fails to boost both cytokine secretion and STAT activation in response to LPS. When the secondary challenge occurs several days after the primary infection with live bacteria, H. pylori-infected monocytes lose their hyper-responsiveness. The observation that H. pylori makes primary human monocytes more susceptible to subsequent/overlapping stimuli provides an important basis to better understand how H. pylori can maintain chronic inflammation and thus contribute to gastric cancer progression. Frontiers Media S.A. 2022-03-02 /pmc/articles/PMC8924073/ /pubmed/35309333 http://dx.doi.org/10.3389/fimmu.2022.847958 Text en Copyright © 2022 Frauenlob, Neuper, Mehinagic, Dang, Boraschi and Horejs-Hoeck https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Frauenlob, Tobias
Neuper, Theresa
Mehinagic, Muamera
Dang, Hieu-Hoa
Boraschi, Diana
Horejs-Hoeck, Jutta
Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title_full Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title_fullStr Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title_full_unstemmed Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title_short Helicobacter pylori Infection of Primary Human Monocytes Boosts Subsequent Immune Responses to LPS
title_sort helicobacter pylori infection of primary human monocytes boosts subsequent immune responses to lps
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924073/
https://www.ncbi.nlm.nih.gov/pubmed/35309333
http://dx.doi.org/10.3389/fimmu.2022.847958
work_keys_str_mv AT frauenlobtobias helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps
AT neupertheresa helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps
AT mehinagicmuamera helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps
AT danghieuhoa helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps
AT boraschidiana helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps
AT horejshoeckjutta helicobacterpyloriinfectionofprimaryhumanmonocytesboostssubsequentimmuneresponsestolps