Scalable and automated CRISPR-based strain engineering using droplet microfluidics

We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip. The microfluidic device contains a 10 × 10 element array, and each element contains sets of electrodes for two electric field-actuated operations: electrowetting for merging...

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Detalles Bibliográficos
Autores principales: Iwai, Kosuke, Wehrs, Maren, Garber, Megan, Sustarich, Jess, Washburn, Lauren, Costello, Zachary, Kim, Peter W., Ando, David, Gaillard, William R., Hillson, Nathan J., Adams, Paul D., Mukhopadhyay, Aindrila, Garcia Martin, Hector, Singh, Anup K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924257/
https://www.ncbi.nlm.nih.gov/pubmed/35359611
http://dx.doi.org/10.1038/s41378-022-00357-3

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