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Genetic polymorphisms of the 5’ untranslated regions of the HSP70 gene in Indonesian cattle populations

BACKGROUND AND AIM: Heat shock proteins (HSPs) are a group of proteins that play a significant role in protecting cells against cellular stress. HSP70 is a conserved, sensitive, and abundant gene associated with heat stress’s physiological adaptability. The objective of this study was to reveal the...

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Detalles Bibliográficos
Autores principales: Prihandini, Peni Wahyu, Primasari, Almira, Aryogi, Aryogi, Luthfi, Muchamad, Hariyono, Dwi Nur Happy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924390/
https://www.ncbi.nlm.nih.gov/pubmed/35369596
http://dx.doi.org/10.14202/vetworld.2022.168-172
Descripción
Sumario:BACKGROUND AND AIM: Heat shock proteins (HSPs) are a group of proteins that play a significant role in protecting cells against cellular stress. HSP70 is a conserved, sensitive, and abundant gene associated with heat stress’s physiological adaptability. The objective of this study was to reveal the polymorphisms of the partial sequences of the HSP70 gene (5’ untranslated region [UTR]) in seven cattle populations in Indonesia. MATERIALS AND METHODS: Polymerase chain reaction products (551 bp) of the HSP70 gene amplified from 102 animals representing seven cattle populations (Bali, Belgian Blue x Peranakan Ongole [PO] cross, Galekan, Jabres, Madura, PO, and Rambon) were sequenced by DNA sequencing method. RESULTS: Fourteen single-nucleotide polymorphisms (SNPs), generally found at a low frequency, were detected. Among these SNPs, only 1117G>A, 1125A>C, and 1204T>C were polymorphic in all the analyzed breeds. A Chi-square test showed that the majority of the loci were in Hardy–Weinberg equilibrium (p>0.05). Varying levels of observed (0.050-0.571) and expected heterozygosity (0.049-0.500) were noted. The polymorphism information content values (0.048-0.375) indicated that the SNPs in the HSP70 gene showed low-to-moderate polymorphism in the studied populations. Thirty-six haplotypes were defined according to the identified SNPs, of which haplotype Hap5 (CGACGAGAGTGTCC) and Hap4 (CGACGAGAGTGCCC) were generally dominant in the studied samples. The phylogenetic tree showed a close relationship between Bali and Rambon cattle and between Galekan and Jabres cattle, while the Belgian Blue x PO crossbred cattle were farther apart. CONCLUSION: The polymorphisms in the 5’ UTR of the HSP70 geneidentified in this study should be further investigated in a larger population to unravel the association between the SNPs and thermotolerance in Indonesian local cattle populations.