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Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase

BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study...

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Autores principales: Hussein, Hassan A., Schuler, Gerhard, Conze, Theresa, Wehrend, Axel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924395/
https://www.ncbi.nlm.nih.gov/pubmed/35369603
http://dx.doi.org/10.14202/vetworld.2022.119-123
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author Hussein, Hassan A.
Schuler, Gerhard
Conze, Theresa
Wehrend, Axel
author_facet Hussein, Hassan A.
Schuler, Gerhard
Conze, Theresa
Wehrend, Axel
author_sort Hussein, Hassan A.
collection PubMed
description BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study aimed to compare two commercial on-site methods (Speed™ P4, Virbac [M1] and mini VIDAS®, bioMérieux [M2]) and a well-established radioimmunoassay (RIA), which was used as a reference method. MATERIALS AND METHODS: Comparative measurements were performed on 52 blood serum samples collected from 45 clinically healthy bitches of different breeds. The dogs had been presented to determine the estrus cycle stage and predict the time of ovulation. Each sample was divided into three aliquots. In aliquot 1, P4 was measured immediately applying M2. Aliquots 2 and 3 were stored at −20°C until analysis was performed using RIA and M1. The consistency of the three methods was investigated by pairwise linear regression analyses. RESULTS: In RIA, the P4 concentrations ranged between 1.1 and 25.4 ng/mL. Regression analyses revealed highly significant (p<0.0001) positive correlations between the three methods applied (M1 vs. RIA: R=0.94; M2 vs. RIA: R=0.98; and M1 vs. M2: R=0.91). CONCLUSION: The results show that the two commercial on-site methods tested exhibit approximately equal, high consistency with the radioimmunological reference method and can, therefore, be used beneficially in a clinical setting. However, biological interpretation of data must be performed in a method-specific manner.
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spelling pubmed-89243952022-04-01 Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase Hussein, Hassan A. Schuler, Gerhard Conze, Theresa Wehrend, Axel Vet World Research Article BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study aimed to compare two commercial on-site methods (Speed™ P4, Virbac [M1] and mini VIDAS®, bioMérieux [M2]) and a well-established radioimmunoassay (RIA), which was used as a reference method. MATERIALS AND METHODS: Comparative measurements were performed on 52 blood serum samples collected from 45 clinically healthy bitches of different breeds. The dogs had been presented to determine the estrus cycle stage and predict the time of ovulation. Each sample was divided into three aliquots. In aliquot 1, P4 was measured immediately applying M2. Aliquots 2 and 3 were stored at −20°C until analysis was performed using RIA and M1. The consistency of the three methods was investigated by pairwise linear regression analyses. RESULTS: In RIA, the P4 concentrations ranged between 1.1 and 25.4 ng/mL. Regression analyses revealed highly significant (p<0.0001) positive correlations between the three methods applied (M1 vs. RIA: R=0.94; M2 vs. RIA: R=0.98; and M1 vs. M2: R=0.91). CONCLUSION: The results show that the two commercial on-site methods tested exhibit approximately equal, high consistency with the radioimmunological reference method and can, therefore, be used beneficially in a clinical setting. However, biological interpretation of data must be performed in a method-specific manner. Veterinary World 2022-01 2022-01-24 /pmc/articles/PMC8924395/ /pubmed/35369603 http://dx.doi.org/10.14202/vetworld.2022.119-123 Text en Copyright: © Hussein, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hussein, Hassan A.
Schuler, Gerhard
Conze, Theresa
Wehrend, Axel
Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title_full Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title_fullStr Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title_full_unstemmed Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title_short Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
title_sort comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924395/
https://www.ncbi.nlm.nih.gov/pubmed/35369603
http://dx.doi.org/10.14202/vetworld.2022.119-123
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