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Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924395/ https://www.ncbi.nlm.nih.gov/pubmed/35369603 http://dx.doi.org/10.14202/vetworld.2022.119-123 |
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author | Hussein, Hassan A. Schuler, Gerhard Conze, Theresa Wehrend, Axel |
author_facet | Hussein, Hassan A. Schuler, Gerhard Conze, Theresa Wehrend, Axel |
author_sort | Hussein, Hassan A. |
collection | PubMed |
description | BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study aimed to compare two commercial on-site methods (Speed™ P4, Virbac [M1] and mini VIDAS®, bioMérieux [M2]) and a well-established radioimmunoassay (RIA), which was used as a reference method. MATERIALS AND METHODS: Comparative measurements were performed on 52 blood serum samples collected from 45 clinically healthy bitches of different breeds. The dogs had been presented to determine the estrus cycle stage and predict the time of ovulation. Each sample was divided into three aliquots. In aliquot 1, P4 was measured immediately applying M2. Aliquots 2 and 3 were stored at −20°C until analysis was performed using RIA and M1. The consistency of the three methods was investigated by pairwise linear regression analyses. RESULTS: In RIA, the P4 concentrations ranged between 1.1 and 25.4 ng/mL. Regression analyses revealed highly significant (p<0.0001) positive correlations between the three methods applied (M1 vs. RIA: R=0.94; M2 vs. RIA: R=0.98; and M1 vs. M2: R=0.91). CONCLUSION: The results show that the two commercial on-site methods tested exhibit approximately equal, high consistency with the radioimmunological reference method and can, therefore, be used beneficially in a clinical setting. However, biological interpretation of data must be performed in a method-specific manner. |
format | Online Article Text |
id | pubmed-8924395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-89243952022-04-01 Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase Hussein, Hassan A. Schuler, Gerhard Conze, Theresa Wehrend, Axel Vet World Research Article BACKGROUND AND AIM: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study aimed to compare two commercial on-site methods (Speed™ P4, Virbac [M1] and mini VIDAS®, bioMérieux [M2]) and a well-established radioimmunoassay (RIA), which was used as a reference method. MATERIALS AND METHODS: Comparative measurements were performed on 52 blood serum samples collected from 45 clinically healthy bitches of different breeds. The dogs had been presented to determine the estrus cycle stage and predict the time of ovulation. Each sample was divided into three aliquots. In aliquot 1, P4 was measured immediately applying M2. Aliquots 2 and 3 were stored at −20°C until analysis was performed using RIA and M1. The consistency of the three methods was investigated by pairwise linear regression analyses. RESULTS: In RIA, the P4 concentrations ranged between 1.1 and 25.4 ng/mL. Regression analyses revealed highly significant (p<0.0001) positive correlations between the three methods applied (M1 vs. RIA: R=0.94; M2 vs. RIA: R=0.98; and M1 vs. M2: R=0.91). CONCLUSION: The results show that the two commercial on-site methods tested exhibit approximately equal, high consistency with the radioimmunological reference method and can, therefore, be used beneficially in a clinical setting. However, biological interpretation of data must be performed in a method-specific manner. Veterinary World 2022-01 2022-01-24 /pmc/articles/PMC8924395/ /pubmed/35369603 http://dx.doi.org/10.14202/vetworld.2022.119-123 Text en Copyright: © Hussein, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hussein, Hassan A. Schuler, Gerhard Conze, Theresa Wehrend, Axel Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title | Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title_full | Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title_fullStr | Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title_full_unstemmed | Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title_short | Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
title_sort | comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924395/ https://www.ncbi.nlm.nih.gov/pubmed/35369603 http://dx.doi.org/10.14202/vetworld.2022.119-123 |
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