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YALIcloneNHEJ: An Efficient Modular Cloning Toolkit for NHEJ Integration of Multigene Pathway and Terpenoid Production in Yarrowia lipolytica
Non-homologous end-joining (NHEJ)-mediated random integration in Yarrowia lipolytica has been demonstrated to be an effective strategy for screening hyperproducer strains. However, there was no multigene assembly method applied for NHEJ integration, which made it challenging to construct and integra...
Autores principales: | Li, Ya-Wen, Yang, Cai-Ling, Shen, Qi, Peng, Qian-Qian, Guo, Qi, Nie, Zhi-Kui, Sun, Xiao-Man, Shi, Tian-Qiong, Ji, Xiao-Jun, Huang, He |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924588/ https://www.ncbi.nlm.nih.gov/pubmed/35308823 http://dx.doi.org/10.3389/fbioe.2021.816980 |
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