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MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway
Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924609/ https://www.ncbi.nlm.nih.gov/pubmed/35310933 http://dx.doi.org/10.1155/2022/3421600 |
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author | Sun, Chen Chen, Chi Chen, Zhen Guo, Jun Yu, Zhi-Hong Qian, Wei Ai, Fen Xiao, Liang Guo, Xiao |
author_facet | Sun, Chen Chen, Chi Chen, Zhen Guo, Jun Yu, Zhi-Hong Qian, Wei Ai, Fen Xiao, Liang Guo, Xiao |
author_sort | Sun, Chen |
collection | PubMed |
description | Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3′-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition notably blocked the tumor-promoting effects of miR-181a-5p. Moreover, we observed that miR-181a-5p mimic further inhibited growth of human osteosarcoma cells in the presence of adriamycin or cisplatin. Overall, miR-181a-5p promotes osteosarcoma progression via PTEN/AKT pathway and it is a promising therapeutic target to treat osteosarcoma. |
format | Online Article Text |
id | pubmed-8924609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-89246092022-03-17 MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway Sun, Chen Chen, Chi Chen, Zhen Guo, Jun Yu, Zhi-Hong Qian, Wei Ai, Fen Xiao, Liang Guo, Xiao Anal Cell Pathol (Amst) Research Article Osteosarcoma is the most common primary malignant bone tumor in children and adolescents with poor prognosis. MicroRNA-181a-5p (miR-181a-5p) is involved in the progression of various tumors; however, its role and underlying mechanism in osteosarcoma remains unclear. In this study, we found that miR-181a-5p was upregulated in human osteosarcoma cells and tissues. miR-181a-5p mimic significantly promoted, while miR-181a-5p inhibitor blocked the proliferation, colony formation, migration, invasion, and cell cycle progression of osteosarcoma cells. Mechanistically, miR-181a-5p bound to the 3′-untranslational region of phosphatase and tensin homolog (PTEN) and reduced its protein expression, thereby activating protein kinase B (PKB/AKT) pathway. Either PTEN overexpression or AKT inhibition notably blocked the tumor-promoting effects of miR-181a-5p. Moreover, we observed that miR-181a-5p mimic further inhibited growth of human osteosarcoma cells in the presence of adriamycin or cisplatin. Overall, miR-181a-5p promotes osteosarcoma progression via PTEN/AKT pathway and it is a promising therapeutic target to treat osteosarcoma. Hindawi 2022-03-08 /pmc/articles/PMC8924609/ /pubmed/35310933 http://dx.doi.org/10.1155/2022/3421600 Text en Copyright © 2022 Chen Sun et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sun, Chen Chen, Chi Chen, Zhen Guo, Jun Yu, Zhi-Hong Qian, Wei Ai, Fen Xiao, Liang Guo, Xiao MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title_full | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title_fullStr | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title_full_unstemmed | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title_short | MicroRNA-181a-5p Promotes Osteosarcoma Progression via PTEN/AKT Pathway |
title_sort | microrna-181a-5p promotes osteosarcoma progression via pten/akt pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924609/ https://www.ncbi.nlm.nih.gov/pubmed/35310933 http://dx.doi.org/10.1155/2022/3421600 |
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