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The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924822/ https://www.ncbi.nlm.nih.gov/pubmed/35316895 http://dx.doi.org/10.5114/aoms/103379 |
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author | Wang, Wei Hua, Tian Li, Xiaodong Zhang, Xinxian Hao, Wei |
author_facet | Wang, Wei Hua, Tian Li, Xiaodong Zhang, Xinxian Hao, Wei |
author_sort | Wang, Wei |
collection | PubMed |
description | INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare the levels of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin. RESULTS: AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than in the PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bind to the irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, treatment with met at an increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose-dependent manner. CONCLUSIONS: In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway. |
format | Online Article Text |
id | pubmed-8924822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-89248222022-03-21 The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome Wang, Wei Hua, Tian Li, Xiaodong Zhang, Xinxian Hao, Wei Arch Med Sci Basic Research INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare the levels of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin. RESULTS: AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than in the PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bind to the irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, treatment with met at an increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose-dependent manner. CONCLUSIONS: In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway. Termedia Publishing House 2021-03-19 /pmc/articles/PMC8924822/ /pubmed/35316895 http://dx.doi.org/10.5114/aoms/103379 Text en Copyright: © 2022 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Basic Research Wang, Wei Hua, Tian Li, Xiaodong Zhang, Xinxian Hao, Wei The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title_full | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title_fullStr | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title_full_unstemmed | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title_short | The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
title_sort | uca1 and microrna-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924822/ https://www.ncbi.nlm.nih.gov/pubmed/35316895 http://dx.doi.org/10.5114/aoms/103379 |
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