Cargando…

The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome

INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare t...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wei, Hua, Tian, Li, Xiaodong, Zhang, Xinxian, Hao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924822/
https://www.ncbi.nlm.nih.gov/pubmed/35316895
http://dx.doi.org/10.5114/aoms/103379
_version_ 1784669941878751232
author Wang, Wei
Hua, Tian
Li, Xiaodong
Zhang, Xinxian
Hao, Wei
author_facet Wang, Wei
Hua, Tian
Li, Xiaodong
Zhang, Xinxian
Hao, Wei
author_sort Wang, Wei
collection PubMed
description INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare the levels of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin. RESULTS: AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than in the PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bind to the irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, treatment with met at an increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose-dependent manner. CONCLUSIONS: In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway.
format Online
Article
Text
id pubmed-8924822
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Termedia Publishing House
record_format MEDLINE/PubMed
spelling pubmed-89248222022-03-21 The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome Wang, Wei Hua, Tian Li, Xiaodong Zhang, Xinxian Hao, Wei Arch Med Sci Basic Research INTRODUCTION: The present study aimed to clarify the underlying mechanism of metformin (met) in the management of polycystic ovary syndrome (PCOS) and to explore the role of the UCA1/microRNA-18a signaling pathway in the control of PCOS. MATERIAL AND METHODS: Real-time PCR was performed to compare the levels of irisin, blood glucose, UCA1 and miR-18a among PCOS, PCOS + Met, and control groups using area under curve (AUC) values. In-silicon analysis and luciferase assay were performed to explore the regulatory relationship among UCA1, miR-18a and irisin. Real-time PCR and Western blot analysis were carried out to detect the effect of met on the expression of UCA1, miR-18a and irisin. RESULTS: AUC of UCA1 was the highest while AUC of irisin was the lowest. Also, irisin and UCA1 levels in the PCOS group were much higher than those in the PCOS + Met group, while miR-18a level in the PCOS group was much lower than in the PCOS + Met group. Through the luciferase assay, miR-18a was proved to directly bind to the irisin 3’UTR. Additionally, irisin was identified to be a target gene of miR-18a. Finally, treatment with met at an increasing concentration reduced the level of UCA1 and irisin but increased the level of miR-18a in a dose-dependent manner. CONCLUSIONS: In the management of PCOS, the irisin-lowering effect of met is regulated by the UCA1/miR-18a/RhoB signaling pathway. Termedia Publishing House 2021-03-19 /pmc/articles/PMC8924822/ /pubmed/35316895 http://dx.doi.org/10.5114/aoms/103379 Text en Copyright: © 2022 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Basic Research
Wang, Wei
Hua, Tian
Li, Xiaodong
Zhang, Xinxian
Hao, Wei
The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title_full The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title_fullStr The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title_full_unstemmed The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title_short The UCA1 and microRNA-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
title_sort uca1 and microrna-18a signaling pathway mediates the irisin-lowering effect of metformin in the management of polycystic ovary syndrome
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924822/
https://www.ncbi.nlm.nih.gov/pubmed/35316895
http://dx.doi.org/10.5114/aoms/103379
work_keys_str_mv AT wangwei theuca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT huatian theuca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT lixiaodong theuca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT zhangxinxian theuca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT haowei theuca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT wangwei uca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT huatian uca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT lixiaodong uca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT zhangxinxian uca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome
AT haowei uca1andmicrorna18asignalingpathwaymediatestheirisinloweringeffectofmetformininthemanagementofpolycysticovarysyndrome