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T-hairpin structure found in the RNA element involved in piRNA biogenesis
PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925976/ https://www.ncbi.nlm.nih.gov/pubmed/34987083 http://dx.doi.org/10.1261/rna.078967.121 |
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author | Takase, Naomi Otsu, Maina Hirakata, Shigeki Ishizu, Hirotsugu Siomi, Mikiko C. Kawai, Gota |
author_facet | Takase, Naomi Otsu, Maina Hirakata, Shigeki Ishizu, Hirotsugu Siomi, Mikiko C. Kawai, Gota |
author_sort | Takase, Naomi |
collection | PubMed |
description | PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesis. In this study, we analyzed the structure of the Tj-cis RNA, a 100-nt RNA corresponding to the Tj-cis element, by the SHAPE and NMR analyses and found that a stable hairpin structure formed in the 5′ half of the Tj-cis RNA. The tertiary structure of the 16-nt stable hairpin was analyzed by NMR, and a novel stem–loop structure, the T-hairpin, was found. In the T-hairpin, four uridine residues are exposed to the solvent, suggesting that this stem–loop is the target of Yb protein, a Tudor domain-containing piRNA biogenesis factor. The piRNA biogenesis assay showed that both the T-hairpin and the 3′ half are required for the function of the Tj-cis element, suggesting that both the T-hairpin and the 3′ half are recognized by Yb protein. |
format | Online Article Text |
id | pubmed-8925976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89259762022-04-01 T-hairpin structure found in the RNA element involved in piRNA biogenesis Takase, Naomi Otsu, Maina Hirakata, Shigeki Ishizu, Hirotsugu Siomi, Mikiko C. Kawai, Gota RNA Article PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesis. In this study, we analyzed the structure of the Tj-cis RNA, a 100-nt RNA corresponding to the Tj-cis element, by the SHAPE and NMR analyses and found that a stable hairpin structure formed in the 5′ half of the Tj-cis RNA. The tertiary structure of the 16-nt stable hairpin was analyzed by NMR, and a novel stem–loop structure, the T-hairpin, was found. In the T-hairpin, four uridine residues are exposed to the solvent, suggesting that this stem–loop is the target of Yb protein, a Tudor domain-containing piRNA biogenesis factor. The piRNA biogenesis assay showed that both the T-hairpin and the 3′ half are required for the function of the Tj-cis element, suggesting that both the T-hairpin and the 3′ half are recognized by Yb protein. Cold Spring Harbor Laboratory Press 2022-04 /pmc/articles/PMC8925976/ /pubmed/34987083 http://dx.doi.org/10.1261/rna.078967.121 Text en © 2022 Takase et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Article Takase, Naomi Otsu, Maina Hirakata, Shigeki Ishizu, Hirotsugu Siomi, Mikiko C. Kawai, Gota T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title | T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title_full | T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title_fullStr | T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title_full_unstemmed | T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title_short | T-hairpin structure found in the RNA element involved in piRNA biogenesis |
title_sort | t-hairpin structure found in the rna element involved in pirna biogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925976/ https://www.ncbi.nlm.nih.gov/pubmed/34987083 http://dx.doi.org/10.1261/rna.078967.121 |
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