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T-hairpin structure found in the RNA element involved in piRNA biogenesis

PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesi...

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Autores principales: Takase, Naomi, Otsu, Maina, Hirakata, Shigeki, Ishizu, Hirotsugu, Siomi, Mikiko C., Kawai, Gota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925976/
https://www.ncbi.nlm.nih.gov/pubmed/34987083
http://dx.doi.org/10.1261/rna.078967.121
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author Takase, Naomi
Otsu, Maina
Hirakata, Shigeki
Ishizu, Hirotsugu
Siomi, Mikiko C.
Kawai, Gota
author_facet Takase, Naomi
Otsu, Maina
Hirakata, Shigeki
Ishizu, Hirotsugu
Siomi, Mikiko C.
Kawai, Gota
author_sort Takase, Naomi
collection PubMed
description PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesis. In this study, we analyzed the structure of the Tj-cis RNA, a 100-nt RNA corresponding to the Tj-cis element, by the SHAPE and NMR analyses and found that a stable hairpin structure formed in the 5′ half of the Tj-cis RNA. The tertiary structure of the 16-nt stable hairpin was analyzed by NMR, and a novel stem–loop structure, the T-hairpin, was found. In the T-hairpin, four uridine residues are exposed to the solvent, suggesting that this stem–loop is the target of Yb protein, a Tudor domain-containing piRNA biogenesis factor. The piRNA biogenesis assay showed that both the T-hairpin and the 3′ half are required for the function of the Tj-cis element, suggesting that both the T-hairpin and the 3′ half are recognized by Yb protein.
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spelling pubmed-89259762022-04-01 T-hairpin structure found in the RNA element involved in piRNA biogenesis Takase, Naomi Otsu, Maina Hirakata, Shigeki Ishizu, Hirotsugu Siomi, Mikiko C. Kawai, Gota RNA Article PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In Drosophila, the Traffic jam (Tj) mRNA is consumed to produce piRNA in its 3′-UTR. A cis element located within the 3′-UTR, Tj-cis, is necessary for piRNA biogenesis. In this study, we analyzed the structure of the Tj-cis RNA, a 100-nt RNA corresponding to the Tj-cis element, by the SHAPE and NMR analyses and found that a stable hairpin structure formed in the 5′ half of the Tj-cis RNA. The tertiary structure of the 16-nt stable hairpin was analyzed by NMR, and a novel stem–loop structure, the T-hairpin, was found. In the T-hairpin, four uridine residues are exposed to the solvent, suggesting that this stem–loop is the target of Yb protein, a Tudor domain-containing piRNA biogenesis factor. The piRNA biogenesis assay showed that both the T-hairpin and the 3′ half are required for the function of the Tj-cis element, suggesting that both the T-hairpin and the 3′ half are recognized by Yb protein. Cold Spring Harbor Laboratory Press 2022-04 /pmc/articles/PMC8925976/ /pubmed/34987083 http://dx.doi.org/10.1261/rna.078967.121 Text en © 2022 Takase et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Article
Takase, Naomi
Otsu, Maina
Hirakata, Shigeki
Ishizu, Hirotsugu
Siomi, Mikiko C.
Kawai, Gota
T-hairpin structure found in the RNA element involved in piRNA biogenesis
title T-hairpin structure found in the RNA element involved in piRNA biogenesis
title_full T-hairpin structure found in the RNA element involved in piRNA biogenesis
title_fullStr T-hairpin structure found in the RNA element involved in piRNA biogenesis
title_full_unstemmed T-hairpin structure found in the RNA element involved in piRNA biogenesis
title_short T-hairpin structure found in the RNA element involved in piRNA biogenesis
title_sort t-hairpin structure found in the rna element involved in pirna biogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8925976/
https://www.ncbi.nlm.nih.gov/pubmed/34987083
http://dx.doi.org/10.1261/rna.078967.121
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