Cargando…
NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized med...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/ https://www.ncbi.nlm.nih.gov/pubmed/35294457 http://dx.doi.org/10.1371/journal.pone.0264897 |
_version_ | 1784670182209224704 |
---|---|
author | Kotzur, Rebecca Duev-Cohen, Alexandra Kol, Inbal Reches, Adi Mandelboim, Ofer Stein, Natan |
author_facet | Kotzur, Rebecca Duev-Cohen, Alexandra Kol, Inbal Reches, Adi Mandelboim, Ofer Stein, Natan |
author_sort | Kotzur, Rebecca |
collection | PubMed |
description | NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies. |
format | Online Article Text |
id | pubmed-8926178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89261782022-03-17 NK-92 cells retain vitality and functionality when grown in standard cell culture conditions Kotzur, Rebecca Duev-Cohen, Alexandra Kol, Inbal Reches, Adi Mandelboim, Ofer Stein, Natan PLoS One Research Article NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies. Public Library of Science 2022-03-16 /pmc/articles/PMC8926178/ /pubmed/35294457 http://dx.doi.org/10.1371/journal.pone.0264897 Text en © 2022 Kotzur et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kotzur, Rebecca Duev-Cohen, Alexandra Kol, Inbal Reches, Adi Mandelboim, Ofer Stein, Natan NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title_full | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title_fullStr | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title_full_unstemmed | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title_short | NK-92 cells retain vitality and functionality when grown in standard cell culture conditions |
title_sort | nk-92 cells retain vitality and functionality when grown in standard cell culture conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/ https://www.ncbi.nlm.nih.gov/pubmed/35294457 http://dx.doi.org/10.1371/journal.pone.0264897 |
work_keys_str_mv | AT kotzurrebecca nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT duevcohenalexandra nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT kolinbal nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT rechesadi nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT mandelboimofer nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions AT steinnatan nk92cellsretainvitalityandfunctionalitywhengrowninstandardcellcultureconditions |