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NK-92 cells retain vitality and functionality when grown in standard cell culture conditions

NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized med...

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Autores principales: Kotzur, Rebecca, Duev-Cohen, Alexandra, Kol, Inbal, Reches, Adi, Mandelboim, Ofer, Stein, Natan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/
https://www.ncbi.nlm.nih.gov/pubmed/35294457
http://dx.doi.org/10.1371/journal.pone.0264897
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author Kotzur, Rebecca
Duev-Cohen, Alexandra
Kol, Inbal
Reches, Adi
Mandelboim, Ofer
Stein, Natan
author_facet Kotzur, Rebecca
Duev-Cohen, Alexandra
Kol, Inbal
Reches, Adi
Mandelboim, Ofer
Stein, Natan
author_sort Kotzur, Rebecca
collection PubMed
description NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies.
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spelling pubmed-89261782022-03-17 NK-92 cells retain vitality and functionality when grown in standard cell culture conditions Kotzur, Rebecca Duev-Cohen, Alexandra Kol, Inbal Reches, Adi Mandelboim, Ofer Stein, Natan PLoS One Research Article NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most ‘NK-like’ cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies. Public Library of Science 2022-03-16 /pmc/articles/PMC8926178/ /pubmed/35294457 http://dx.doi.org/10.1371/journal.pone.0264897 Text en © 2022 Kotzur et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kotzur, Rebecca
Duev-Cohen, Alexandra
Kol, Inbal
Reches, Adi
Mandelboim, Ofer
Stein, Natan
NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title_full NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title_fullStr NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title_full_unstemmed NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title_short NK-92 cells retain vitality and functionality when grown in standard cell culture conditions
title_sort nk-92 cells retain vitality and functionality when grown in standard cell culture conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926178/
https://www.ncbi.nlm.nih.gov/pubmed/35294457
http://dx.doi.org/10.1371/journal.pone.0264897
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