Salivary surprise: Symmerista caterpillars anoint petioles with red saliva after clipping leaves

After feeding on a tree leaf, caterpillars in ten families sever the petiole and allow the remaining leaf fragment to fall to the ground. Previous researchers proposed that the caterpillars thereby reduced bird predation by eliminating visual evidence of feeding. In this study, 26 species of caterpillars in five families were filmed clipping leaves. Caterpillar behavior did not conform to the visual cue hypothesis. Some caterpillars clipped midribs and petioles repeatedly even though a single clip would suffice to reduce visual cues for birds. Every caterpillar that clipped a leaf rubbed its spinneret (which secretes saliva from the labial glands) over the petiole or midrib stub. In the notodontids Symmerista albifrons and S. leucitys, petiole stubs were bathed in red fluid. Cauterizing the spinneret eliminated fluid application. Dissections documented that the anterior portion of their labial glands contained red pigment, thereby confirming that the red secretion is saliva. When applied to petiole stubs, the red pigment in Symmerista saliva travelled several mm in five minutes within the petiole xylem demonstrating the potential for rapid movement of salivary constituents into the plant. In diverse caterpillars, including species that clip leaves, saliva contains substances reported to suppress plant defenses. Thus, leaf clipping likely functions primarily not to remove visual cues, but to introduce salivary constituents into the plant that prevent defenses from being mobilized in nearby leaves where the caterpillar feeds next.