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Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy

Calorie restriction (CR) and the activation of autophagy extend healthspan by delaying the onset of age-associated diseases in most living organisms. Because protein kinase CK2 (CK2) downregulation induces cellular senescence and nematode aging, we investigated CK2’s role in CR and autophagy. This s...

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Autores principales: Park, Jeong-Woo, Jeong, Jihyeon, Bae, Young-Seuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926869/
https://www.ncbi.nlm.nih.gov/pubmed/34949740
http://dx.doi.org/10.14348/molcells.2021.0183
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author Park, Jeong-Woo
Jeong, Jihyeon
Bae, Young-Seuk
author_facet Park, Jeong-Woo
Jeong, Jihyeon
Bae, Young-Seuk
author_sort Park, Jeong-Woo
collection PubMed
description Calorie restriction (CR) and the activation of autophagy extend healthspan by delaying the onset of age-associated diseases in most living organisms. Because protein kinase CK2 (CK2) downregulation induces cellular senescence and nematode aging, we investigated CK2’s role in CR and autophagy. This study indicated that CR upregulated CK2’s expression, thereby causing SIRT1 and AMP-activated protein kinase (AMPK) activation. CK2α overexpression, including antisense inhibitors of miR-186, miR-216b, miR-337-3p, and miR-760, stimulated autophagy initiation and nucleation markers (increase in ATG5, ATG7, LC3BII, beclin-1, and Ulk1, and decrease in SQSTM1/p62). The SIRT1 deacetylase, AKT, mammalian target of rapamycin (mTOR), AMPK, and forkhead homeobox type O (FoxO) 3a were involved in CK2-mediated autophagy. The treatment with the AKT inhibitor triciribine, the AMPK activator AICAR, or the SIRT1 activator resveratrol rescued a reduction in the expression of lgg-1 (the Caenorhabditis elegans ortholog of LC3B), bec-1 (the C. elegans ortholog of beclin-1), and unc-51 (the C. elegans ortholog of Ulk1), mediated by kin-10 (the C. elegans ortholog of CK2β) knockdown in nematodes. Thus, this study indicated that CK2 acted as a positive regulator in CR and autophagy, thereby suggesting that these four miRs’ antisense inhibitors can be used as CR mimetics or autophagy inducers.
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spelling pubmed-89268692022-03-28 Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy Park, Jeong-Woo Jeong, Jihyeon Bae, Young-Seuk Mol Cells Research Article Calorie restriction (CR) and the activation of autophagy extend healthspan by delaying the onset of age-associated diseases in most living organisms. Because protein kinase CK2 (CK2) downregulation induces cellular senescence and nematode aging, we investigated CK2’s role in CR and autophagy. This study indicated that CR upregulated CK2’s expression, thereby causing SIRT1 and AMP-activated protein kinase (AMPK) activation. CK2α overexpression, including antisense inhibitors of miR-186, miR-216b, miR-337-3p, and miR-760, stimulated autophagy initiation and nucleation markers (increase in ATG5, ATG7, LC3BII, beclin-1, and Ulk1, and decrease in SQSTM1/p62). The SIRT1 deacetylase, AKT, mammalian target of rapamycin (mTOR), AMPK, and forkhead homeobox type O (FoxO) 3a were involved in CK2-mediated autophagy. The treatment with the AKT inhibitor triciribine, the AMPK activator AICAR, or the SIRT1 activator resveratrol rescued a reduction in the expression of lgg-1 (the Caenorhabditis elegans ortholog of LC3B), bec-1 (the C. elegans ortholog of beclin-1), and unc-51 (the C. elegans ortholog of Ulk1), mediated by kin-10 (the C. elegans ortholog of CK2β) knockdown in nematodes. Thus, this study indicated that CK2 acted as a positive regulator in CR and autophagy, thereby suggesting that these four miRs’ antisense inhibitors can be used as CR mimetics or autophagy inducers. Korean Society for Molecular and Cellular Biology 2022-03-31 2021-12-15 /pmc/articles/PMC8926869/ /pubmed/34949740 http://dx.doi.org/10.14348/molcells.2021.0183 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/)
spellingShingle Research Article
Park, Jeong-Woo
Jeong, Jihyeon
Bae, Young-Seuk
Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title_full Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title_fullStr Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title_full_unstemmed Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title_short Protein Kinase CK2 Is Upregulated by Calorie Restriction and Induces Autophagy
title_sort protein kinase ck2 is upregulated by calorie restriction and induces autophagy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926869/
https://www.ncbi.nlm.nih.gov/pubmed/34949740
http://dx.doi.org/10.14348/molcells.2021.0183
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