Cargando…

Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma

The composition of the plasma membrane (PM)-associated proteome of tumor cells determines cell–cell and cell–matrix interactions and the response to environmental cues. Whether the PM-associated proteome impacts the phenotype of Medulloblastoma (MB) tumor cells and how it adapts in response to growt...

Descripción completa

Detalles Bibliográficos
Autores principales: Capdeville, Charles, Russo, Linda, Penton, David, Migliavacca, Jessica, Zecevic, Milica, Gries, Alexandre, Neuhauss, Stephan CF, Grotzer, Michael A, Baumgartner, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
https://www.ncbi.nlm.nih.gov/pubmed/35296518
http://dx.doi.org/10.26508/lsa.202201380
_version_ 1784670339384475648
author Capdeville, Charles
Russo, Linda
Penton, David
Migliavacca, Jessica
Zecevic, Milica
Gries, Alexandre
Neuhauss, Stephan CF
Grotzer, Michael A
Baumgartner, Martin
author_facet Capdeville, Charles
Russo, Linda
Penton, David
Migliavacca, Jessica
Zecevic, Milica
Gries, Alexandre
Neuhauss, Stephan CF
Grotzer, Michael A
Baumgartner, Martin
author_sort Capdeville, Charles
collection PubMed
description The composition of the plasma membrane (PM)-associated proteome of tumor cells determines cell–cell and cell–matrix interactions and the response to environmental cues. Whether the PM-associated proteome impacts the phenotype of Medulloblastoma (MB) tumor cells and how it adapts in response to growth factor cues is poorly understood. Using a spatial proteomics approach, we observed that hepatocyte growth factor (HGF)-induced activation of the receptor tyrosine kinase c-MET in MB cells changes the abundance of transmembrane and membrane-associated proteins. The depletion of MAP4K4, a pro-migratory effector kinase downstream of c-MET, leads to a specific decrease of the adhesion and immunomodulatory receptor CD155 and of components of the fast-endophilin–mediated endocytosis (FEME) machinery in the PM-associated proteome of HGF-activated MB cells. The decreased surface expression of CD155 or of the fast-endophilin–mediated endocytosis effector endophilin-A1 reduces growth and invasiveness of MB tumor cells in the tissue context. These data thus describe a novel function of MAP4K4 in the control of the PM-associated proteome of tumor cells and identified two downstream effector mechanisms controlling proliferation and invasiveness of MB cells.
format Online
Article
Text
id pubmed-8926928
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Life Science Alliance LLC
record_format MEDLINE/PubMed
spelling pubmed-89269282022-04-11 Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma Capdeville, Charles Russo, Linda Penton, David Migliavacca, Jessica Zecevic, Milica Gries, Alexandre Neuhauss, Stephan CF Grotzer, Michael A Baumgartner, Martin Life Sci Alliance Research Articles The composition of the plasma membrane (PM)-associated proteome of tumor cells determines cell–cell and cell–matrix interactions and the response to environmental cues. Whether the PM-associated proteome impacts the phenotype of Medulloblastoma (MB) tumor cells and how it adapts in response to growth factor cues is poorly understood. Using a spatial proteomics approach, we observed that hepatocyte growth factor (HGF)-induced activation of the receptor tyrosine kinase c-MET in MB cells changes the abundance of transmembrane and membrane-associated proteins. The depletion of MAP4K4, a pro-migratory effector kinase downstream of c-MET, leads to a specific decrease of the adhesion and immunomodulatory receptor CD155 and of components of the fast-endophilin–mediated endocytosis (FEME) machinery in the PM-associated proteome of HGF-activated MB cells. The decreased surface expression of CD155 or of the fast-endophilin–mediated endocytosis effector endophilin-A1 reduces growth and invasiveness of MB tumor cells in the tissue context. These data thus describe a novel function of MAP4K4 in the control of the PM-associated proteome of tumor cells and identified two downstream effector mechanisms controlling proliferation and invasiveness of MB cells. Life Science Alliance LLC 2022-03-16 /pmc/articles/PMC8926928/ /pubmed/35296518 http://dx.doi.org/10.26508/lsa.202201380 Text en © 2022 Capdeville et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Capdeville, Charles
Russo, Linda
Penton, David
Migliavacca, Jessica
Zecevic, Milica
Gries, Alexandre
Neuhauss, Stephan CF
Grotzer, Michael A
Baumgartner, Martin
Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title_full Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title_fullStr Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title_full_unstemmed Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title_short Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
title_sort spatial proteomics finds cd155 and endophilin-a1 as mediators of growth and invasion in medulloblastoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
https://www.ncbi.nlm.nih.gov/pubmed/35296518
http://dx.doi.org/10.26508/lsa.202201380
work_keys_str_mv AT capdevillecharles spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT russolinda spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT pentondavid spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT migliavaccajessica spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT zecevicmilica spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT griesalexandre spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT neuhaussstephancf spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT grotzermichaela spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma
AT baumgartnermartin spatialproteomicsfindscd155andendophilina1asmediatorsofgrowthandinvasioninmedulloblastoma