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Bias at the third nucleotide of codon pairs in virus and host genomes
Genomes of different sizes and complexity can be compared using common features. Most genomes contain open reading frames, and most genomes use the same genetic code. Redundancy in the genetic code means that different biases in the third nucleotide position of a codon exist in different genomes. Ho...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927144/ https://www.ncbi.nlm.nih.gov/pubmed/35296743 http://dx.doi.org/10.1038/s41598-022-08570-w |
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author | Plant, Ewan P. Ye, Zhiping |
author_facet | Plant, Ewan P. Ye, Zhiping |
author_sort | Plant, Ewan P. |
collection | PubMed |
description | Genomes of different sizes and complexity can be compared using common features. Most genomes contain open reading frames, and most genomes use the same genetic code. Redundancy in the genetic code means that different biases in the third nucleotide position of a codon exist in different genomes. However, the nucleotide composition of viruses can be quite different from host nucleotide composition making it difficult to assess the relevance of these biases. Here we show that grouping codons of a codon-pair according to the GC content of the first two nucleotide positions of each codon reveals patterns in nucleotide usage at the third position of the 1st codon. Differences between the observed and expected biases occur predominantly when the first two nucleotides of the 2nd codon are both S (strong, G or C) or both W (weak, A or T), not a mixture of strong and weak. The data indicates that some codon pairs are preferred because of the strength of the interactions between the codon and anticodon, the adjacent tRNAs and the ribosome. Using base-pairing strength and third position bias facilitates the comparison of genomes of different size and nucleotide composition and reveals patterns not previously described. |
format | Online Article Text |
id | pubmed-8927144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89271442022-03-17 Bias at the third nucleotide of codon pairs in virus and host genomes Plant, Ewan P. Ye, Zhiping Sci Rep Article Genomes of different sizes and complexity can be compared using common features. Most genomes contain open reading frames, and most genomes use the same genetic code. Redundancy in the genetic code means that different biases in the third nucleotide position of a codon exist in different genomes. However, the nucleotide composition of viruses can be quite different from host nucleotide composition making it difficult to assess the relevance of these biases. Here we show that grouping codons of a codon-pair according to the GC content of the first two nucleotide positions of each codon reveals patterns in nucleotide usage at the third position of the 1st codon. Differences between the observed and expected biases occur predominantly when the first two nucleotides of the 2nd codon are both S (strong, G or C) or both W (weak, A or T), not a mixture of strong and weak. The data indicates that some codon pairs are preferred because of the strength of the interactions between the codon and anticodon, the adjacent tRNAs and the ribosome. Using base-pairing strength and third position bias facilitates the comparison of genomes of different size and nucleotide composition and reveals patterns not previously described. Nature Publishing Group UK 2022-03-16 /pmc/articles/PMC8927144/ /pubmed/35296743 http://dx.doi.org/10.1038/s41598-022-08570-w Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Plant, Ewan P. Ye, Zhiping Bias at the third nucleotide of codon pairs in virus and host genomes |
title | Bias at the third nucleotide of codon pairs in virus and host genomes |
title_full | Bias at the third nucleotide of codon pairs in virus and host genomes |
title_fullStr | Bias at the third nucleotide of codon pairs in virus and host genomes |
title_full_unstemmed | Bias at the third nucleotide of codon pairs in virus and host genomes |
title_short | Bias at the third nucleotide of codon pairs in virus and host genomes |
title_sort | bias at the third nucleotide of codon pairs in virus and host genomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927144/ https://www.ncbi.nlm.nih.gov/pubmed/35296743 http://dx.doi.org/10.1038/s41598-022-08570-w |
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