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VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance
Anticoagulant rodenticides are commonly used in rodent control because they are economical and have great deployment versatility. However, rodents with Single Nucleotide Polymorphism (SNP) mutations within the Vkorc1 gene are resistant to the effects of anticoagulant rodenticide use and this influen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927331/ https://www.ncbi.nlm.nih.gov/pubmed/35296766 http://dx.doi.org/10.1038/s41598-022-08653-8 |
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author | Chua, Cliff Humaidi, Mahathir Neves, Erica Sena Mailepessov, Diyar Ng, Lee Ching Aik, Joel |
author_facet | Chua, Cliff Humaidi, Mahathir Neves, Erica Sena Mailepessov, Diyar Ng, Lee Ching Aik, Joel |
author_sort | Chua, Cliff |
collection | PubMed |
description | Anticoagulant rodenticides are commonly used in rodent control because they are economical and have great deployment versatility. However, rodents with Single Nucleotide Polymorphism (SNP) mutations within the Vkorc1 gene are resistant to the effects of anticoagulant rodenticide use and this influences the effectiveness of control strategies that rely on such rodenticides. This study examined the prevalence of rat SNP mutations in Singapore to inform the effectiveness of anticoagulant rodenticide use. A total of 130 rat tail samples, comprising 83 Rattus norvegicus (63.8%) and 47 Rattus rattus complex (36.2%) were conveniently sampled from November 2016 to December 2019 from urban settings and sequenced at exon 3 of Vkorc1. Sequencing analysis revealed 4 synonymous and 1 non-synonymous mutations in Rattus rattus complex samples. A novel synonymous mutation of L108L was identified and not previously reported in other studies. Non-synonymous SNPs were not detected in the notable codons of 120, 128 and 139 in R. norvegicus, where these regions are internationally recognised to be associated with resistance from prior studies. Our findings suggest that the prevalence of anticoagulant rodenticide resistance in Singapore is low. Continued monitoring of rodenticide resistance is important for informing rodent control strategies aimed at reducing rodent-borne disease transmission. |
format | Online Article Text |
id | pubmed-8927331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89273312022-03-17 VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance Chua, Cliff Humaidi, Mahathir Neves, Erica Sena Mailepessov, Diyar Ng, Lee Ching Aik, Joel Sci Rep Article Anticoagulant rodenticides are commonly used in rodent control because they are economical and have great deployment versatility. However, rodents with Single Nucleotide Polymorphism (SNP) mutations within the Vkorc1 gene are resistant to the effects of anticoagulant rodenticide use and this influences the effectiveness of control strategies that rely on such rodenticides. This study examined the prevalence of rat SNP mutations in Singapore to inform the effectiveness of anticoagulant rodenticide use. A total of 130 rat tail samples, comprising 83 Rattus norvegicus (63.8%) and 47 Rattus rattus complex (36.2%) were conveniently sampled from November 2016 to December 2019 from urban settings and sequenced at exon 3 of Vkorc1. Sequencing analysis revealed 4 synonymous and 1 non-synonymous mutations in Rattus rattus complex samples. A novel synonymous mutation of L108L was identified and not previously reported in other studies. Non-synonymous SNPs were not detected in the notable codons of 120, 128 and 139 in R. norvegicus, where these regions are internationally recognised to be associated with resistance from prior studies. Our findings suggest that the prevalence of anticoagulant rodenticide resistance in Singapore is low. Continued monitoring of rodenticide resistance is important for informing rodent control strategies aimed at reducing rodent-borne disease transmission. Nature Publishing Group UK 2022-03-16 /pmc/articles/PMC8927331/ /pubmed/35296766 http://dx.doi.org/10.1038/s41598-022-08653-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chua, Cliff Humaidi, Mahathir Neves, Erica Sena Mailepessov, Diyar Ng, Lee Ching Aik, Joel VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title | VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title_full | VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title_fullStr | VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title_full_unstemmed | VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title_short | VKORC1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
title_sort | vkorc1 mutations in rodent populations of a tropical city-state as an indicator of anticoagulant rodenticide resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927331/ https://www.ncbi.nlm.nih.gov/pubmed/35296766 http://dx.doi.org/10.1038/s41598-022-08653-8 |
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