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D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm

Peri-implantitis is the leading cause of dental implant failure, initially raised by biofilm accumulation on the implant surface. During the development of biofilm, Actinomyces viscosus (A. viscosus) plays a pivotal role in initial attachment as well as the bacterial coaggregation of multispecies pa...

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Autores principales: Li, Baosheng, Cai, Qing, Wang, Zixuan, Qiao, Shuwei, Ou, Yanzhen, Ma, Rui, Luo, Chuanfu, Meng, Weiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927782/
https://www.ncbi.nlm.nih.gov/pubmed/35309977
http://dx.doi.org/10.3389/fbioe.2022.864012
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author Li, Baosheng
Cai, Qing
Wang, Zixuan
Qiao, Shuwei
Ou, Yanzhen
Ma, Rui
Luo, Chuanfu
Meng, Weiyan
author_facet Li, Baosheng
Cai, Qing
Wang, Zixuan
Qiao, Shuwei
Ou, Yanzhen
Ma, Rui
Luo, Chuanfu
Meng, Weiyan
author_sort Li, Baosheng
collection PubMed
description Peri-implantitis is the leading cause of dental implant failure, initially raised by biofilm accumulation on the implant surface. During the development of biofilm, Actinomyces viscosus (A. viscosus) plays a pivotal role in initial attachment as well as the bacterial coaggregation of multispecies pathogens. Hence, eliminating the A. viscosus-associated biofilm is fundamental for the regeneration of the lost bone around implants. Whereas clinical evidence indicated that antimicrobials and debridement did not show significant effects on the decontamination of biofilm on the implant surface. In this study, alpha-amylase was investigated for its effects on disassembling A. viscosus biofilm. Then, in order to substantially disperse biofilm under biosafety concentration, D-arginine was employed to appraise its enhancing effects on alpha-amylase. In addition, molecular dynamics simulations and molecular docking were conducted to elucidate the mechanism of D-arginine enhancing alpha-amylase. 0.1–0.5% alpha-amylase showed significant effects on disassembling A. viscosus biofilm, with definite cytotoxicity toward MC3T3-E1 cells meanwhile. Intriguingly, 8 mM D-arginine drastically enhanced the eradication of A. viscosus biofilm biomass by 0.01% alpha-amylase with biosafety in 30 min. The exopolysaccharides of biofilm were also thoroughly hydrolyzed by 0.01% alpha-amylase with 8 mM D-arginine. The biofilm thickness and integrity were disrupted, and the exopolysaccharides among the extracellular matrix were elusive. Molecular dynamics simulations showed that with the hydrogen bonding of D-arginine to the catalytic triad and calcium-binding regions of alpha-amylase, the atom fluctuation of the structure was attenuated. The distances between catalytic triad were shortened, and the calcium-binding regions became more stable. Molecular docking scores revealed that D-arginine facilitated the maltotetraose binding process of alpha-amylase. In conclusion, these results demonstrate that D-arginine enhances the disassembly effects of alpha-amylase on A. viscosus biofilm through potentiating the catalytic triad and stabilizing the calcium-binding regions, thus providing a novel strategy for the decontamination of biofilm contaminated implant surface.
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spelling pubmed-89277822022-03-18 D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm Li, Baosheng Cai, Qing Wang, Zixuan Qiao, Shuwei Ou, Yanzhen Ma, Rui Luo, Chuanfu Meng, Weiyan Front Bioeng Biotechnol Bioengineering and Biotechnology Peri-implantitis is the leading cause of dental implant failure, initially raised by biofilm accumulation on the implant surface. During the development of biofilm, Actinomyces viscosus (A. viscosus) plays a pivotal role in initial attachment as well as the bacterial coaggregation of multispecies pathogens. Hence, eliminating the A. viscosus-associated biofilm is fundamental for the regeneration of the lost bone around implants. Whereas clinical evidence indicated that antimicrobials and debridement did not show significant effects on the decontamination of biofilm on the implant surface. In this study, alpha-amylase was investigated for its effects on disassembling A. viscosus biofilm. Then, in order to substantially disperse biofilm under biosafety concentration, D-arginine was employed to appraise its enhancing effects on alpha-amylase. In addition, molecular dynamics simulations and molecular docking were conducted to elucidate the mechanism of D-arginine enhancing alpha-amylase. 0.1–0.5% alpha-amylase showed significant effects on disassembling A. viscosus biofilm, with definite cytotoxicity toward MC3T3-E1 cells meanwhile. Intriguingly, 8 mM D-arginine drastically enhanced the eradication of A. viscosus biofilm biomass by 0.01% alpha-amylase with biosafety in 30 min. The exopolysaccharides of biofilm were also thoroughly hydrolyzed by 0.01% alpha-amylase with 8 mM D-arginine. The biofilm thickness and integrity were disrupted, and the exopolysaccharides among the extracellular matrix were elusive. Molecular dynamics simulations showed that with the hydrogen bonding of D-arginine to the catalytic triad and calcium-binding regions of alpha-amylase, the atom fluctuation of the structure was attenuated. The distances between catalytic triad were shortened, and the calcium-binding regions became more stable. Molecular docking scores revealed that D-arginine facilitated the maltotetraose binding process of alpha-amylase. In conclusion, these results demonstrate that D-arginine enhances the disassembly effects of alpha-amylase on A. viscosus biofilm through potentiating the catalytic triad and stabilizing the calcium-binding regions, thus providing a novel strategy for the decontamination of biofilm contaminated implant surface. Frontiers Media S.A. 2022-03-03 /pmc/articles/PMC8927782/ /pubmed/35309977 http://dx.doi.org/10.3389/fbioe.2022.864012 Text en Copyright © 2022 Li, Cai, Wang, Qiao, Ou, Ma, Luo and Meng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Baosheng
Cai, Qing
Wang, Zixuan
Qiao, Shuwei
Ou, Yanzhen
Ma, Rui
Luo, Chuanfu
Meng, Weiyan
D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title_full D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title_fullStr D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title_full_unstemmed D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title_short D-arginine Enhances the Effect of Alpha-Amylase on Disassembling Actinomyces viscosus Biofilm
title_sort d-arginine enhances the effect of alpha-amylase on disassembling actinomyces viscosus biofilm
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927782/
https://www.ncbi.nlm.nih.gov/pubmed/35309977
http://dx.doi.org/10.3389/fbioe.2022.864012
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