Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus

Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strat...

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Detalles Bibliográficos
Autores principales: Lax, Carlos, Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, Navarro, Eusebio, Nicolás, Francisco Esteban, Garre, Victoriano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927829/
https://www.ncbi.nlm.nih.gov/pubmed/35308131
http://dx.doi.org/10.1016/j.xpro.2022.101237
Descripción
Sumario:Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of R. microsporus and will be useful for the study of mucormycosis. For complete details on the use and execution of this protocol, please refer to Lax et al. (2021).

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