Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus

Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strat...

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Detalles Bibliográficos
Autores principales: Lax, Carlos, Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, Navarro, Eusebio, Nicolás, Francisco Esteban, Garre, Victoriano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927829/
https://www.ncbi.nlm.nih.gov/pubmed/35308131
http://dx.doi.org/10.1016/j.xpro.2022.101237
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author Lax, Carlos
Navarro-Mendoza, María Isabel
Pérez-Arques, Carlos
Navarro, Eusebio
Nicolás, Francisco Esteban
Garre, Victoriano
author_facet Lax, Carlos
Navarro-Mendoza, María Isabel
Pérez-Arques, Carlos
Navarro, Eusebio
Nicolás, Francisco Esteban
Garre, Victoriano
author_sort Lax, Carlos
collection PubMed
description Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of R. microsporus and will be useful for the study of mucormycosis. For complete details on the use and execution of this protocol, please refer to Lax et al. (2021).
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spelling pubmed-89278292022-03-18 Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus Lax, Carlos Navarro-Mendoza, María Isabel Pérez-Arques, Carlos Navarro, Eusebio Nicolás, Francisco Esteban Garre, Victoriano STAR Protoc Protocol Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of R. microsporus and will be useful for the study of mucormycosis. For complete details on the use and execution of this protocol, please refer to Lax et al. (2021). Elsevier 2022-03-14 /pmc/articles/PMC8927829/ /pubmed/35308131 http://dx.doi.org/10.1016/j.xpro.2022.101237 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lax, Carlos
Navarro-Mendoza, María Isabel
Pérez-Arques, Carlos
Navarro, Eusebio
Nicolás, Francisco Esteban
Garre, Victoriano
Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title_full Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title_fullStr Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title_full_unstemmed Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title_short Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
title_sort transformation and crispr-cas9-mediated homologous recombination in the fungus rhizopus microsporus
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927829/
https://www.ncbi.nlm.nih.gov/pubmed/35308131
http://dx.doi.org/10.1016/j.xpro.2022.101237
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