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Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus

Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strat...

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Detalles Bibliográficos
Autores principales:	Lax, Carlos, Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, Navarro, Eusebio, Nicolás, Francisco Esteban, Garre, Victoriano
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927829/ https://www.ncbi.nlm.nih.gov/pubmed/35308131 http://dx.doi.org/10.1016/j.xpro.2022.101237

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