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Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928453/ https://www.ncbi.nlm.nih.gov/pubmed/34525360 http://dx.doi.org/10.1016/j.celrep.2021.109703 |
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author | Ulloa, Bianca A. Habbsa, Samima S. Potts, Kathryn S. Lewis, Alana McKinstry, Mia Payne, Sara G. Flores, Julio C. Nizhnik, Anastasia Norberto, Maria Feliz Mosimann, Christian Bowman, Teresa V. |
author_facet | Ulloa, Bianca A. Habbsa, Samima S. Potts, Kathryn S. Lewis, Alana McKinstry, Mia Payne, Sara G. Flores, Julio C. Nizhnik, Anastasia Norberto, Maria Feliz Mosimann, Christian Bowman, Teresa V. |
author_sort | Ulloa, Bianca A. |
collection | PubMed |
description | Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene expression with other embryonic blood progenitors. Using single-cell transcriptomics, we define gene signatures that distinguish nascent HSCs from embryonic blood progenitors. Applying a lineage-tracing approach to selectively track HSC output in situ, we find significantly delayed lymphomyeloid contribution. An inducible HSC injury model demonstrates a negligible impact on larval lymphomyelopoiesis following HSC depletion. HSCs are not merely dormant at this developmental stage, as they showed robust regeneration after injury. Combined, our findings illuminate that nascent HSCs self-renew but display differentiation latency, while HSC-independent embryonic progenitors sustain developmental hematopoiesis. Understanding these differences could improve de novo generation and expansion of functional HSCs. |
format | Online Article Text |
id | pubmed-8928453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-89284532022-03-17 Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis Ulloa, Bianca A. Habbsa, Samima S. Potts, Kathryn S. Lewis, Alana McKinstry, Mia Payne, Sara G. Flores, Julio C. Nizhnik, Anastasia Norberto, Maria Feliz Mosimann, Christian Bowman, Teresa V. Cell Rep Article Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene expression with other embryonic blood progenitors. Using single-cell transcriptomics, we define gene signatures that distinguish nascent HSCs from embryonic blood progenitors. Applying a lineage-tracing approach to selectively track HSC output in situ, we find significantly delayed lymphomyeloid contribution. An inducible HSC injury model demonstrates a negligible impact on larval lymphomyelopoiesis following HSC depletion. HSCs are not merely dormant at this developmental stage, as they showed robust regeneration after injury. Combined, our findings illuminate that nascent HSCs self-renew but display differentiation latency, while HSC-independent embryonic progenitors sustain developmental hematopoiesis. Understanding these differences could improve de novo generation and expansion of functional HSCs. 2021-09-14 /pmc/articles/PMC8928453/ /pubmed/34525360 http://dx.doi.org/10.1016/j.celrep.2021.109703 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Ulloa, Bianca A. Habbsa, Samima S. Potts, Kathryn S. Lewis, Alana McKinstry, Mia Payne, Sara G. Flores, Julio C. Nizhnik, Anastasia Norberto, Maria Feliz Mosimann, Christian Bowman, Teresa V. Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis |
title | Definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
title_full | Definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
title_fullStr | Definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
title_full_unstemmed | Definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
title_short | Definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
title_sort | definitive hematopoietic stem cells minimally contribute to embryonic
hematopoiesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928453/ https://www.ncbi.nlm.nih.gov/pubmed/34525360 http://dx.doi.org/10.1016/j.celrep.2021.109703 |
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