Cargando…

Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis

Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene...

Descripción completa

Detalles Bibliográficos
Autores principales: Ulloa, Bianca A., Habbsa, Samima S., Potts, Kathryn S., Lewis, Alana, McKinstry, Mia, Payne, Sara G., Flores, Julio C., Nizhnik, Anastasia, Norberto, Maria Feliz, Mosimann, Christian, Bowman, Teresa V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928453/
https://www.ncbi.nlm.nih.gov/pubmed/34525360
http://dx.doi.org/10.1016/j.celrep.2021.109703
_version_ 1784670644421525504
author Ulloa, Bianca A.
Habbsa, Samima S.
Potts, Kathryn S.
Lewis, Alana
McKinstry, Mia
Payne, Sara G.
Flores, Julio C.
Nizhnik, Anastasia
Norberto, Maria Feliz
Mosimann, Christian
Bowman, Teresa V.
author_facet Ulloa, Bianca A.
Habbsa, Samima S.
Potts, Kathryn S.
Lewis, Alana
McKinstry, Mia
Payne, Sara G.
Flores, Julio C.
Nizhnik, Anastasia
Norberto, Maria Feliz
Mosimann, Christian
Bowman, Teresa V.
author_sort Ulloa, Bianca A.
collection PubMed
description Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene expression with other embryonic blood progenitors. Using single-cell transcriptomics, we define gene signatures that distinguish nascent HSCs from embryonic blood progenitors. Applying a lineage-tracing approach to selectively track HSC output in situ, we find significantly delayed lymphomyeloid contribution. An inducible HSC injury model demonstrates a negligible impact on larval lymphomyelopoiesis following HSC depletion. HSCs are not merely dormant at this developmental stage, as they showed robust regeneration after injury. Combined, our findings illuminate that nascent HSCs self-renew but display differentiation latency, while HSC-independent embryonic progenitors sustain developmental hematopoiesis. Understanding these differences could improve de novo generation and expansion of functional HSCs.
format Online
Article
Text
id pubmed-8928453
institution National Center for Biotechnology Information
language English
publishDate 2021
record_format MEDLINE/PubMed
spelling pubmed-89284532022-03-17 Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis Ulloa, Bianca A. Habbsa, Samima S. Potts, Kathryn S. Lewis, Alana McKinstry, Mia Payne, Sara G. Flores, Julio C. Nizhnik, Anastasia Norberto, Maria Feliz Mosimann, Christian Bowman, Teresa V. Cell Rep Article Hematopoietic stem cells (HSCs) are rare cells that arise in the embryo and sustain adult hematopoiesis. Although the functional potential of nascent HSCs is detectable by transplantation, their native contribution during development is unknown, in part due to the overlapping genesis and marker gene expression with other embryonic blood progenitors. Using single-cell transcriptomics, we define gene signatures that distinguish nascent HSCs from embryonic blood progenitors. Applying a lineage-tracing approach to selectively track HSC output in situ, we find significantly delayed lymphomyeloid contribution. An inducible HSC injury model demonstrates a negligible impact on larval lymphomyelopoiesis following HSC depletion. HSCs are not merely dormant at this developmental stage, as they showed robust regeneration after injury. Combined, our findings illuminate that nascent HSCs self-renew but display differentiation latency, while HSC-independent embryonic progenitors sustain developmental hematopoiesis. Understanding these differences could improve de novo generation and expansion of functional HSCs. 2021-09-14 /pmc/articles/PMC8928453/ /pubmed/34525360 http://dx.doi.org/10.1016/j.celrep.2021.109703 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Ulloa, Bianca A.
Habbsa, Samima S.
Potts, Kathryn S.
Lewis, Alana
McKinstry, Mia
Payne, Sara G.
Flores, Julio C.
Nizhnik, Anastasia
Norberto, Maria Feliz
Mosimann, Christian
Bowman, Teresa V.
Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title_full Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title_fullStr Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title_full_unstemmed Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title_short Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
title_sort definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928453/
https://www.ncbi.nlm.nih.gov/pubmed/34525360
http://dx.doi.org/10.1016/j.celrep.2021.109703
work_keys_str_mv AT ulloabiancaa definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT habbsasamimas definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT pottskathryns definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT lewisalana definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT mckinstrymia definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT paynesarag definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT floresjulioc definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT nizhnikanastasia definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT norbertomariafeliz definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT mosimannchristian definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis
AT bowmanteresav definitivehematopoieticstemcellsminimallycontributetoembryonichematopoiesis