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Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function

[Image: see text] A considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following compl...

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Autores principales: Ghosh, Susmita, Parikh, Swapneil, Nissa, Mehar Un, Acharjee, Arup, Singh, Avinash, Patwa, Dhruv, Makwana, Prashant, Athalye, Arundhati, Barpanda, Abhilash, Laloraya, Malini, Srivastava, Sanjeeva, Parikh, Firuza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928495/
https://www.ncbi.nlm.nih.gov/pubmed/35309488
http://dx.doi.org/10.1021/acsomega.1c06551
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author Ghosh, Susmita
Parikh, Swapneil
Nissa, Mehar Un
Acharjee, Arup
Singh, Avinash
Patwa, Dhruv
Makwana, Prashant
Athalye, Arundhati
Barpanda, Abhilash
Laloraya, Malini
Srivastava, Sanjeeva
Parikh, Firuza
author_facet Ghosh, Susmita
Parikh, Swapneil
Nissa, Mehar Un
Acharjee, Arup
Singh, Avinash
Patwa, Dhruv
Makwana, Prashant
Athalye, Arundhati
Barpanda, Abhilash
Laloraya, Malini
Srivastava, Sanjeeva
Parikh, Firuza
author_sort Ghosh, Susmita
collection PubMed
description [Image: see text] A considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following complete clinical recovery using mass spectrometry. A label-free quantitative proteomics study involved 10 healthy fertile subjects and 17 COVID-19-recovered men. With 1% false discovery rate and >1 unique peptide stringency, MaxQuant analysis found 1099 proteins and 8503 peptides. Of the 48 differentially expressed proteins between the healthy and COVID-19-recovered groups, 21 proteins were downregulated and 27 were upregulated in COVID-19-recovered males. The major pathways involved in reproductive functions, such as sperm–oocyte recognition, testosterone response, cell motility regulation, adhesion regulation, extracellular matrix adhesion, and endopeptidase activity, were downregulated in COVID-19-recovered patients according to bioinformatics analysis. Furthermore, the targeted approach revealed significant downregulation of semenogelin 1 and prosaposin, two proteins related to male fertility. Therefore, we demonstrate the alteration of semen proteome in response to COVID-19, thus disrupting the male reproductive function despite the patient’s clinical remission. Hence, to understand fertility-related biological processes triggered by this infection, a protracted evaluation of the consequences of COVID-19 in recovered men is warranted.
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spelling pubmed-89284952022-03-18 Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function Ghosh, Susmita Parikh, Swapneil Nissa, Mehar Un Acharjee, Arup Singh, Avinash Patwa, Dhruv Makwana, Prashant Athalye, Arundhati Barpanda, Abhilash Laloraya, Malini Srivastava, Sanjeeva Parikh, Firuza ACS Omega [Image: see text] A considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following complete clinical recovery using mass spectrometry. A label-free quantitative proteomics study involved 10 healthy fertile subjects and 17 COVID-19-recovered men. With 1% false discovery rate and >1 unique peptide stringency, MaxQuant analysis found 1099 proteins and 8503 peptides. Of the 48 differentially expressed proteins between the healthy and COVID-19-recovered groups, 21 proteins were downregulated and 27 were upregulated in COVID-19-recovered males. The major pathways involved in reproductive functions, such as sperm–oocyte recognition, testosterone response, cell motility regulation, adhesion regulation, extracellular matrix adhesion, and endopeptidase activity, were downregulated in COVID-19-recovered patients according to bioinformatics analysis. Furthermore, the targeted approach revealed significant downregulation of semenogelin 1 and prosaposin, two proteins related to male fertility. Therefore, we demonstrate the alteration of semen proteome in response to COVID-19, thus disrupting the male reproductive function despite the patient’s clinical remission. Hence, to understand fertility-related biological processes triggered by this infection, a protracted evaluation of the consequences of COVID-19 in recovered men is warranted. American Chemical Society 2022-03-07 /pmc/articles/PMC8928495/ /pubmed/35309488 http://dx.doi.org/10.1021/acsomega.1c06551 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Ghosh, Susmita
Parikh, Swapneil
Nissa, Mehar Un
Acharjee, Arup
Singh, Avinash
Patwa, Dhruv
Makwana, Prashant
Athalye, Arundhati
Barpanda, Abhilash
Laloraya, Malini
Srivastava, Sanjeeva
Parikh, Firuza
Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title_full Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title_fullStr Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title_full_unstemmed Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title_short Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
title_sort semen proteomics of covid-19 convalescent men reveals disruption of key biological pathways relevant to male reproductive function
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928495/
https://www.ncbi.nlm.nih.gov/pubmed/35309488
http://dx.doi.org/10.1021/acsomega.1c06551
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