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Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928568/ https://www.ncbi.nlm.nih.gov/pubmed/35310294 http://dx.doi.org/10.1093/ve/veab105 |
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author | Tschá, Marcel Kruchelski Suzukawa, Andreia A Rodrigues-Luiz, Gabriela Flavia da Silva, Allan Martins Cataneo, Allan Henrique Depieri Mattoso Coelho, Gabriela Ferreira, Adão Celestino Soares Medeiros, Lia Carolina Mansur, Daniel Zanluca, Camila Duarte dos Santos, Claudia N |
author_facet | Tschá, Marcel Kruchelski Suzukawa, Andreia A Rodrigues-Luiz, Gabriela Flavia da Silva, Allan Martins Cataneo, Allan Henrique Depieri Mattoso Coelho, Gabriela Ferreira, Adão Celestino Soares Medeiros, Lia Carolina Mansur, Daniel Zanluca, Camila Duarte dos Santos, Claudia N |
author_sort | Tschá, Marcel Kruchelski |
collection | PubMed |
description | Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected in a rural area of Piraí do Sul, South Brazil. In vitro assays revealed that PIRAV replicates and causes cytopathic effects in vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 and UMNSAH/DF-1. Genomic signature analysis supports these results showing a dinucleotide and codon usage balance compatible with several hosts. Phylogenetic analyses placed PIRAV basal to the Venezuelan equine encephalitis complex. Genome analyses, electron microscopy, and biological characterization show findings that may alert for the emergence of a new arbovirus in South America. |
format | Online Article Text |
id | pubmed-8928568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89285682022-03-18 Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil Tschá, Marcel Kruchelski Suzukawa, Andreia A Rodrigues-Luiz, Gabriela Flavia da Silva, Allan Martins Cataneo, Allan Henrique Depieri Mattoso Coelho, Gabriela Ferreira, Adão Celestino Soares Medeiros, Lia Carolina Mansur, Daniel Zanluca, Camila Duarte dos Santos, Claudia N Virus Evol Research Article Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected in a rural area of Piraí do Sul, South Brazil. In vitro assays revealed that PIRAV replicates and causes cytopathic effects in vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 and UMNSAH/DF-1. Genomic signature analysis supports these results showing a dinucleotide and codon usage balance compatible with several hosts. Phylogenetic analyses placed PIRAV basal to the Venezuelan equine encephalitis complex. Genome analyses, electron microscopy, and biological characterization show findings that may alert for the emergence of a new arbovirus in South America. Oxford University Press 2021-12-17 /pmc/articles/PMC8928568/ /pubmed/35310294 http://dx.doi.org/10.1093/ve/veab105 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Tschá, Marcel Kruchelski Suzukawa, Andreia A Rodrigues-Luiz, Gabriela Flavia da Silva, Allan Martins Cataneo, Allan Henrique Depieri Mattoso Coelho, Gabriela Ferreira, Adão Celestino Soares Medeiros, Lia Carolina Mansur, Daniel Zanluca, Camila Duarte dos Santos, Claudia N Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil |
title | Pirahy virus: Identification of a new and potential emerging arbovirus in
South Brazil |
title_full | Pirahy virus: Identification of a new and potential emerging arbovirus in
South Brazil |
title_fullStr | Pirahy virus: Identification of a new and potential emerging arbovirus in
South Brazil |
title_full_unstemmed | Pirahy virus: Identification of a new and potential emerging arbovirus in
South Brazil |
title_short | Pirahy virus: Identification of a new and potential emerging arbovirus in
South Brazil |
title_sort | pirahy virus: identification of a new and potential emerging arbovirus in
south brazil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928568/ https://www.ncbi.nlm.nih.gov/pubmed/35310294 http://dx.doi.org/10.1093/ve/veab105 |
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