Cargando…

Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil

Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from...

Descripción completa

Detalles Bibliográficos
Autores principales: Tschá, Marcel Kruchelski, Suzukawa, Andreia A, Rodrigues-Luiz, Gabriela Flavia, da Silva, Allan Martins, Cataneo, Allan Henrique Depieri, Mattoso Coelho, Gabriela, Ferreira, Adão Celestino, Soares Medeiros, Lia Carolina, Mansur, Daniel, Zanluca, Camila, Duarte dos Santos, Claudia N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928568/
https://www.ncbi.nlm.nih.gov/pubmed/35310294
http://dx.doi.org/10.1093/ve/veab105
_version_ 1784670670963081216
author Tschá, Marcel Kruchelski
Suzukawa, Andreia A
Rodrigues-Luiz, Gabriela Flavia
da Silva, Allan Martins
Cataneo, Allan Henrique Depieri
Mattoso Coelho, Gabriela
Ferreira, Adão Celestino
Soares Medeiros, Lia Carolina
Mansur, Daniel
Zanluca, Camila
Duarte dos Santos, Claudia N
author_facet Tschá, Marcel Kruchelski
Suzukawa, Andreia A
Rodrigues-Luiz, Gabriela Flavia
da Silva, Allan Martins
Cataneo, Allan Henrique Depieri
Mattoso Coelho, Gabriela
Ferreira, Adão Celestino
Soares Medeiros, Lia Carolina
Mansur, Daniel
Zanluca, Camila
Duarte dos Santos, Claudia N
author_sort Tschá, Marcel Kruchelski
collection PubMed
description Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected in a rural area of Piraí do Sul, South Brazil. In vitro assays revealed that PIRAV replicates and causes cytopathic effects in vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 and UMNSAH/DF-1. Genomic signature analysis supports these results showing a dinucleotide and codon usage balance compatible with several hosts. Phylogenetic analyses placed PIRAV basal to the Venezuelan equine encephalitis complex. Genome analyses, electron microscopy, and biological characterization show findings that may alert for the emergence of a new arbovirus in South America.
format Online
Article
Text
id pubmed-8928568
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-89285682022-03-18 Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil Tschá, Marcel Kruchelski Suzukawa, Andreia A Rodrigues-Luiz, Gabriela Flavia da Silva, Allan Martins Cataneo, Allan Henrique Depieri Mattoso Coelho, Gabriela Ferreira, Adão Celestino Soares Medeiros, Lia Carolina Mansur, Daniel Zanluca, Camila Duarte dos Santos, Claudia N Virus Evol Research Article Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected in a rural area of Piraí do Sul, South Brazil. In vitro assays revealed that PIRAV replicates and causes cytopathic effects in vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 and UMNSAH/DF-1. Genomic signature analysis supports these results showing a dinucleotide and codon usage balance compatible with several hosts. Phylogenetic analyses placed PIRAV basal to the Venezuelan equine encephalitis complex. Genome analyses, electron microscopy, and biological characterization show findings that may alert for the emergence of a new arbovirus in South America. Oxford University Press 2021-12-17 /pmc/articles/PMC8928568/ /pubmed/35310294 http://dx.doi.org/10.1093/ve/veab105 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Tschá, Marcel Kruchelski
Suzukawa, Andreia A
Rodrigues-Luiz, Gabriela Flavia
da Silva, Allan Martins
Cataneo, Allan Henrique Depieri
Mattoso Coelho, Gabriela
Ferreira, Adão Celestino
Soares Medeiros, Lia Carolina
Mansur, Daniel
Zanluca, Camila
Duarte dos Santos, Claudia N
Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title_full Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title_fullStr Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title_full_unstemmed Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title_short Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil
title_sort pirahy virus: identification of a new and potential emerging arbovirus in south brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928568/
https://www.ncbi.nlm.nih.gov/pubmed/35310294
http://dx.doi.org/10.1093/ve/veab105
work_keys_str_mv AT tschamarcelkruchelski pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT suzukawaandreiaa pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT rodriguesluizgabrielaflavia pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT dasilvaallanmartins pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT cataneoallanhenriquedepieri pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT mattosocoelhogabriela pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT ferreiraadaocelestino pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT soaresmedeirosliacarolina pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT mansurdaniel pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT zanlucacamila pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil
AT duartedossantosclaudian pirahyvirusidentificationofanewandpotentialemergingarbovirusinsouthbrazil