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Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer

The aim of the study was to develop a new diagnostic biomarker for identifying serum exosomal miRNAs specific to epithelial ovarian cancer (EOC) and to find out target gene of the miRNA for exploring the molecular mechanisms in EOC. A total of 84 cases of ovarian masses and sera were enrolled, compr...

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Autores principales: Jeon, Hyeji, Seo, Su Min, Kim, Tae Wan, Ryu, Jaesung, Kong, Hyejeong, Jang, Si Hyeong, Jang, Yong Soo, Kim, Kwang Seock, Kim, Jae Hoon, Ryu, Seongho, Jeon, Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928998/
https://www.ncbi.nlm.nih.gov/pubmed/35723400
http://dx.doi.org/10.3390/cimb44010021
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author Jeon, Hyeji
Seo, Su Min
Kim, Tae Wan
Ryu, Jaesung
Kong, Hyejeong
Jang, Si Hyeong
Jang, Yong Soo
Kim, Kwang Seock
Kim, Jae Hoon
Ryu, Seongho
Jeon, Seob
author_facet Jeon, Hyeji
Seo, Su Min
Kim, Tae Wan
Ryu, Jaesung
Kong, Hyejeong
Jang, Si Hyeong
Jang, Yong Soo
Kim, Kwang Seock
Kim, Jae Hoon
Ryu, Seongho
Jeon, Seob
author_sort Jeon, Hyeji
collection PubMed
description The aim of the study was to develop a new diagnostic biomarker for identifying serum exosomal miRNAs specific to epithelial ovarian cancer (EOC) and to find out target gene of the miRNA for exploring the molecular mechanisms in EOC. A total of 84 cases of ovarian masses and sera were enrolled, comprising EOC (n = 71), benign ovarian neoplasms (n = 13). We detected expression of candidate miRNAs in the serum and tissue of both benign ovarian neoplasm group and EOC group using real-time polymerase chain reaction. Immunohistochemistry were constructed using formalin fixed paraffin embedded (FFPE) tissue to detect expression level of suppressor of cytokine signaling 4 (SOCS4). In the EOC group, miRNA-1290 was significantly overexpressed in serum exosomes and tissues as compared to benign ovarian neoplasm group (fold change ≥ 2, p < 0.05). We observed area under the receiver operating characteristic curve (AUC) for miR-1290, using a cut-off of 0.73, the exosomal miR-1290 from serum had AUC, sensitivity, and specificity values of 0.794, 69.2 and 87.3, respectively. In immunohistochemical study, expression of SOCS4 in EOC was lower than that in benign ovarian neoplasm. Serum exosomal miR-1290 could be considered as a biomarker for differential diagnosis of EOC from benign ovarian neoplasm and SOCS4 might be potential target gene of miR-1290 in EOC.
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spelling pubmed-89289982022-06-04 Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer Jeon, Hyeji Seo, Su Min Kim, Tae Wan Ryu, Jaesung Kong, Hyejeong Jang, Si Hyeong Jang, Yong Soo Kim, Kwang Seock Kim, Jae Hoon Ryu, Seongho Jeon, Seob Curr Issues Mol Biol Article The aim of the study was to develop a new diagnostic biomarker for identifying serum exosomal miRNAs specific to epithelial ovarian cancer (EOC) and to find out target gene of the miRNA for exploring the molecular mechanisms in EOC. A total of 84 cases of ovarian masses and sera were enrolled, comprising EOC (n = 71), benign ovarian neoplasms (n = 13). We detected expression of candidate miRNAs in the serum and tissue of both benign ovarian neoplasm group and EOC group using real-time polymerase chain reaction. Immunohistochemistry were constructed using formalin fixed paraffin embedded (FFPE) tissue to detect expression level of suppressor of cytokine signaling 4 (SOCS4). In the EOC group, miRNA-1290 was significantly overexpressed in serum exosomes and tissues as compared to benign ovarian neoplasm group (fold change ≥ 2, p < 0.05). We observed area under the receiver operating characteristic curve (AUC) for miR-1290, using a cut-off of 0.73, the exosomal miR-1290 from serum had AUC, sensitivity, and specificity values of 0.794, 69.2 and 87.3, respectively. In immunohistochemical study, expression of SOCS4 in EOC was lower than that in benign ovarian neoplasm. Serum exosomal miR-1290 could be considered as a biomarker for differential diagnosis of EOC from benign ovarian neoplasm and SOCS4 might be potential target gene of miR-1290 in EOC. MDPI 2022-01-09 /pmc/articles/PMC8928998/ /pubmed/35723400 http://dx.doi.org/10.3390/cimb44010021 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jeon, Hyeji
Seo, Su Min
Kim, Tae Wan
Ryu, Jaesung
Kong, Hyejeong
Jang, Si Hyeong
Jang, Yong Soo
Kim, Kwang Seock
Kim, Jae Hoon
Ryu, Seongho
Jeon, Seob
Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title_full Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title_fullStr Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title_full_unstemmed Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title_short Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer
title_sort circulating exosomal mir-1290 for diagnosis of epithelial ovarian cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928998/
https://www.ncbi.nlm.nih.gov/pubmed/35723400
http://dx.doi.org/10.3390/cimb44010021
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