Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay qu...

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Autores principales: John, Goldin, Sahajpal, Nikhil Shri, Mondal, Ashis K., Ananth, Sudha, Williams, Colin, Chaubey, Alka, Rojiani, Amyn M., Kolhe, Ravindra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929009/
https://www.ncbi.nlm.nih.gov/pubmed/34449545
http://dx.doi.org/10.3390/cimb43020061
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author John, Goldin
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Ananth, Sudha
Williams, Colin
Chaubey, Alka
Rojiani, Amyn M.
Kolhe, Ravindra
author_facet John, Goldin
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Ananth, Sudha
Williams, Colin
Chaubey, Alka
Rojiani, Amyn M.
Kolhe, Ravindra
author_sort John, Goldin
collection PubMed
description This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
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spelling pubmed-89290092022-06-04 Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology John, Goldin Sahajpal, Nikhil Shri Mondal, Ashis K. Ananth, Sudha Williams, Colin Chaubey, Alka Rojiani, Amyn M. Kolhe, Ravindra Curr Issues Mol Biol Review This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19. MDPI 2021-07-30 /pmc/articles/PMC8929009/ /pubmed/34449545 http://dx.doi.org/10.3390/cimb43020061 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
John, Goldin
Sahajpal, Nikhil Shri
Mondal, Ashis K.
Ananth, Sudha
Williams, Colin
Chaubey, Alka
Rojiani, Amyn M.
Kolhe, Ravindra
Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title_full Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title_fullStr Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title_full_unstemmed Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title_short Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
title_sort next-generation sequencing (ngs) in covid-19: a tool for sars-cov-2 diagnosis, monitoring new strains and phylodynamic modeling in molecular epidemiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929009/
https://www.ncbi.nlm.nih.gov/pubmed/34449545
http://dx.doi.org/10.3390/cimb43020061
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