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Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach

Kawasaki disease (KD) typically occurs in children aged under 5 years and can cause coronary artery lesions (CALs). Early diagnosis and treatment with intravenous immunoglobulin can reduce the occurrence of CALs; therefore, identifying a good biomarker for diagnosing KD is essential. Here, using nex...

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Autores principales: Weng, Ken-Pen, Cheng, Ching-Feng, Chien, Kuang-Jen, Ger, Luo-Ping, Huang, Shih-Hui, Tsai, Kuo-Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929010/
https://www.ncbi.nlm.nih.gov/pubmed/34202375
http://dx.doi.org/10.3390/cimb43020037
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author Weng, Ken-Pen
Cheng, Ching-Feng
Chien, Kuang-Jen
Ger, Luo-Ping
Huang, Shih-Hui
Tsai, Kuo-Wang
author_facet Weng, Ken-Pen
Cheng, Ching-Feng
Chien, Kuang-Jen
Ger, Luo-Ping
Huang, Shih-Hui
Tsai, Kuo-Wang
author_sort Weng, Ken-Pen
collection PubMed
description Kawasaki disease (KD) typically occurs in children aged under 5 years and can cause coronary artery lesions (CALs). Early diagnosis and treatment with intravenous immunoglobulin can reduce the occurrence of CALs; therefore, identifying a good biomarker for diagnosing KD is essential. Here, using next-generation sequencing in patients with recurrent KD, those with viral infection, and healthy controls, we identified dysregulated circulating microRNAs as diagnostic biomarkers for KD. Pathway enrichment analysis illustrated the putative role of these miRNAs in KD progression. Their expression levels were validated using real-time polymerase chain reaction (qPCR). Fifteen dysregulated circulating miRNAs (fold changes >2 and <0.5) were differentially expressed in the recurrent KD group compared with the viral infection and control groups. These miRNAs were significantly involved in the transforming growth factor-β, epithelial–mesenchymal transition, and cell apoptosis signaling pathways. Notably, their expression levels were frequently restored after intravenous immunoglobulin treatment. Among the candidates, miR-24-3p expression level was significantly higher in patients with recurrent KD compared with healthy controls or viral infection controls (p < 0.001). Receiver operating characteristic analysis revealed that high miR-24-3p expression levels may be a potential biomarker for KD diagnosis. In conclusion, we identified miR-24-3p significantly higher in KD patients, which may be a potential diagnostic biomarker for KD.
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spelling pubmed-89290102022-06-04 Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach Weng, Ken-Pen Cheng, Ching-Feng Chien, Kuang-Jen Ger, Luo-Ping Huang, Shih-Hui Tsai, Kuo-Wang Curr Issues Mol Biol Article Kawasaki disease (KD) typically occurs in children aged under 5 years and can cause coronary artery lesions (CALs). Early diagnosis and treatment with intravenous immunoglobulin can reduce the occurrence of CALs; therefore, identifying a good biomarker for diagnosing KD is essential. Here, using next-generation sequencing in patients with recurrent KD, those with viral infection, and healthy controls, we identified dysregulated circulating microRNAs as diagnostic biomarkers for KD. Pathway enrichment analysis illustrated the putative role of these miRNAs in KD progression. Their expression levels were validated using real-time polymerase chain reaction (qPCR). Fifteen dysregulated circulating miRNAs (fold changes >2 and <0.5) were differentially expressed in the recurrent KD group compared with the viral infection and control groups. These miRNAs were significantly involved in the transforming growth factor-β, epithelial–mesenchymal transition, and cell apoptosis signaling pathways. Notably, their expression levels were frequently restored after intravenous immunoglobulin treatment. Among the candidates, miR-24-3p expression level was significantly higher in patients with recurrent KD compared with healthy controls or viral infection controls (p < 0.001). Receiver operating characteristic analysis revealed that high miR-24-3p expression levels may be a potential biomarker for KD diagnosis. In conclusion, we identified miR-24-3p significantly higher in KD patients, which may be a potential diagnostic biomarker for KD. MDPI 2021-06-25 /pmc/articles/PMC8929010/ /pubmed/34202375 http://dx.doi.org/10.3390/cimb43020037 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weng, Ken-Pen
Cheng, Ching-Feng
Chien, Kuang-Jen
Ger, Luo-Ping
Huang, Shih-Hui
Tsai, Kuo-Wang
Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title_full Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title_fullStr Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title_full_unstemmed Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title_short Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
title_sort identifying circulating microrna in kawasaki disease by next-generation sequencing approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929010/
https://www.ncbi.nlm.nih.gov/pubmed/34202375
http://dx.doi.org/10.3390/cimb43020037
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