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A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance

Risk factors for type 2 diabetes mellitus (T2DM) consist of a combination of an unhealthy, imbalanced diet and genetic factors that may interact with each other. Single nucleotide polymorphism (SNP) in the prospero homeobox 1 (PROX1) gene is a strong genetic susceptibility factor for this metabolic...

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Autores principales: Mojsak, Patrycja, Miniewska, Katarzyna, Godlewski, Adrian, Adamska-Patruno, Edyta, Samczuk, Paulina, Rey-Stolle, Fernanda, Bauer, Witold, Barbas, Coral, Kretowski, Adam, Ciborowski, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929026/
https://www.ncbi.nlm.nih.gov/pubmed/34209638
http://dx.doi.org/10.3390/cimb43020039
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author Mojsak, Patrycja
Miniewska, Katarzyna
Godlewski, Adrian
Adamska-Patruno, Edyta
Samczuk, Paulina
Rey-Stolle, Fernanda
Bauer, Witold
Barbas, Coral
Kretowski, Adam
Ciborowski, Michal
author_facet Mojsak, Patrycja
Miniewska, Katarzyna
Godlewski, Adrian
Adamska-Patruno, Edyta
Samczuk, Paulina
Rey-Stolle, Fernanda
Bauer, Witold
Barbas, Coral
Kretowski, Adam
Ciborowski, Michal
author_sort Mojsak, Patrycja
collection PubMed
description Risk factors for type 2 diabetes mellitus (T2DM) consist of a combination of an unhealthy, imbalanced diet and genetic factors that may interact with each other. Single nucleotide polymorphism (SNP) in the prospero homeobox 1 (PROX1) gene is a strong genetic susceptibility factor for this metabolic disorder and impaired β-cell function. As the role of this gene in T2DM development remains unclear, novel approaches are needed to advance the understanding of the mechanisms of T2DM development. Therefore, in this study, for the first time, postprandial changes in plasma metabolites were analysed by GC–MS in nondiabetic men with different PROX1 genotypes up to 5 years prior to prediabetes appearance. Eighteen contestants (12 with high risk (HR) and 6 with low risk (LR) genotype) participated in high-carbohydrate (HC) and normo-carbohydrate (NC) meal-challenge tests. Our study concluded that both meal-challenge tests provoked changes in 15 plasma metabolites (amino acids, carbohydrates, fatty acids and others) in HR, but not LR genotype carriers. Postprandial changes in the levels of some of the detected metabolites may be a source of potential specific early disturbances possibly associated with the future development of T2DM. Thus, accurate determination of these metabolites can be important for the early diagnosis of this metabolic disease.
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spelling pubmed-89290262022-06-04 A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance Mojsak, Patrycja Miniewska, Katarzyna Godlewski, Adrian Adamska-Patruno, Edyta Samczuk, Paulina Rey-Stolle, Fernanda Bauer, Witold Barbas, Coral Kretowski, Adam Ciborowski, Michal Curr Issues Mol Biol Article Risk factors for type 2 diabetes mellitus (T2DM) consist of a combination of an unhealthy, imbalanced diet and genetic factors that may interact with each other. Single nucleotide polymorphism (SNP) in the prospero homeobox 1 (PROX1) gene is a strong genetic susceptibility factor for this metabolic disorder and impaired β-cell function. As the role of this gene in T2DM development remains unclear, novel approaches are needed to advance the understanding of the mechanisms of T2DM development. Therefore, in this study, for the first time, postprandial changes in plasma metabolites were analysed by GC–MS in nondiabetic men with different PROX1 genotypes up to 5 years prior to prediabetes appearance. Eighteen contestants (12 with high risk (HR) and 6 with low risk (LR) genotype) participated in high-carbohydrate (HC) and normo-carbohydrate (NC) meal-challenge tests. Our study concluded that both meal-challenge tests provoked changes in 15 plasma metabolites (amino acids, carbohydrates, fatty acids and others) in HR, but not LR genotype carriers. Postprandial changes in the levels of some of the detected metabolites may be a source of potential specific early disturbances possibly associated with the future development of T2DM. Thus, accurate determination of these metabolites can be important for the early diagnosis of this metabolic disease. MDPI 2021-06-29 /pmc/articles/PMC8929026/ /pubmed/34209638 http://dx.doi.org/10.3390/cimb43020039 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mojsak, Patrycja
Miniewska, Katarzyna
Godlewski, Adrian
Adamska-Patruno, Edyta
Samczuk, Paulina
Rey-Stolle, Fernanda
Bauer, Witold
Barbas, Coral
Kretowski, Adam
Ciborowski, Michal
A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title_full A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title_fullStr A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title_full_unstemmed A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title_short A Preliminary Study Showing the Impact of Genetic and Dietary Factors on GC–MS-Based Plasma Metabolome of Patients with and without PROX1-Genetic Predisposition to T2DM up to 5 Years Prior to Prediabetes Appearance
title_sort preliminary study showing the impact of genetic and dietary factors on gc–ms-based plasma metabolome of patients with and without prox1-genetic predisposition to t2dm up to 5 years prior to prediabetes appearance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929026/
https://www.ncbi.nlm.nih.gov/pubmed/34209638
http://dx.doi.org/10.3390/cimb43020039
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