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Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells

Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca ce...

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Autores principales: Dai, Truong Xuan, Son, Hoang Nghia, Chi, Ho Nguyen Quynh, Huy, Hoang Nghia Quang, Minh, Nguyen Thai, Tram, Nguyen Thi Thuy, Huyen, Nguyen Thi Thuong, Quan, To Minh, Chung, Doan Chinh, Nhung, Truong Hai, Minh, Tran Thi, Diem, Tran Hong, Mai, Nguyen Thi Phuong, Long, Le Thanh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929043/
https://www.ncbi.nlm.nih.gov/pubmed/34940129
http://dx.doi.org/10.3390/cimb43030155
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author Dai, Truong Xuan
Son, Hoang Nghia
Chi, Ho Nguyen Quynh
Huy, Hoang Nghia Quang
Minh, Nguyen Thai
Tram, Nguyen Thi Thuy
Huyen, Nguyen Thi Thuong
Quan, To Minh
Chung, Doan Chinh
Nhung, Truong Hai
Minh, Tran Thi
Diem, Tran Hong
Mai, Nguyen Thi Phuong
Long, Le Thanh
author_facet Dai, Truong Xuan
Son, Hoang Nghia
Chi, Ho Nguyen Quynh
Huy, Hoang Nghia Quang
Minh, Nguyen Thai
Tram, Nguyen Thi Thuy
Huyen, Nguyen Thi Thuong
Quan, To Minh
Chung, Doan Chinh
Nhung, Truong Hai
Minh, Tran Thi
Diem, Tran Hong
Mai, Nguyen Thi Phuong
Long, Le Thanh
author_sort Dai, Truong Xuan
collection PubMed
description Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca cells. However, the effects of microgravity on these cells have not been well characterized, especially in granulosa cells. This study aimed to investigate the effects of simulated microgravity (SMG) on the proliferation and morphology of porcine granulosa cells (pGCs). pGC proliferation from the SMG group was inhibited, demonstrated by the reduced O.D. value and cell density in the WST-1 assay and cell number counting. SMG-induced pGCs exhibited an increased ratio of cells in the G0/G1 phase and a decreased ratio of cells in the S and G2/M phase. Western blot analysis indicated a down-regulation of cyclin D1, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 6 (cdk6), leading to the prevention of the G1-S transition and inducing the arrest phase. pGCs under the SMG condition showed an increase in nuclear area. This caused a reduction in nuclear shape value in pGCs under the SMG condition. SMG-induced pGCs exhibited different morphologies, including fibroblast-like shape, rhomboid shape, and pebble-like shape. These results revealed that SMG inhibited proliferation and induced morphological changes in pGCs.
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spelling pubmed-89290432022-06-04 Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells Dai, Truong Xuan Son, Hoang Nghia Chi, Ho Nguyen Quynh Huy, Hoang Nghia Quang Minh, Nguyen Thai Tram, Nguyen Thi Thuy Huyen, Nguyen Thi Thuong Quan, To Minh Chung, Doan Chinh Nhung, Truong Hai Minh, Tran Thi Diem, Tran Hong Mai, Nguyen Thi Phuong Long, Le Thanh Curr Issues Mol Biol Article Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca cells. However, the effects of microgravity on these cells have not been well characterized, especially in granulosa cells. This study aimed to investigate the effects of simulated microgravity (SMG) on the proliferation and morphology of porcine granulosa cells (pGCs). pGC proliferation from the SMG group was inhibited, demonstrated by the reduced O.D. value and cell density in the WST-1 assay and cell number counting. SMG-induced pGCs exhibited an increased ratio of cells in the G0/G1 phase and a decreased ratio of cells in the S and G2/M phase. Western blot analysis indicated a down-regulation of cyclin D1, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 6 (cdk6), leading to the prevention of the G1-S transition and inducing the arrest phase. pGCs under the SMG condition showed an increase in nuclear area. This caused a reduction in nuclear shape value in pGCs under the SMG condition. SMG-induced pGCs exhibited different morphologies, including fibroblast-like shape, rhomboid shape, and pebble-like shape. These results revealed that SMG inhibited proliferation and induced morphological changes in pGCs. MDPI 2021-12-10 /pmc/articles/PMC8929043/ /pubmed/34940129 http://dx.doi.org/10.3390/cimb43030155 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dai, Truong Xuan
Son, Hoang Nghia
Chi, Ho Nguyen Quynh
Huy, Hoang Nghia Quang
Minh, Nguyen Thai
Tram, Nguyen Thi Thuy
Huyen, Nguyen Thi Thuong
Quan, To Minh
Chung, Doan Chinh
Nhung, Truong Hai
Minh, Tran Thi
Diem, Tran Hong
Mai, Nguyen Thi Phuong
Long, Le Thanh
Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title_full Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title_fullStr Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title_full_unstemmed Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title_short Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
title_sort simulated microgravity induces the proliferative inhibition and morphological changes in porcine granulosa cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929043/
https://www.ncbi.nlm.nih.gov/pubmed/34940129
http://dx.doi.org/10.3390/cimb43030155
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