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Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells
Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca ce...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929043/ https://www.ncbi.nlm.nih.gov/pubmed/34940129 http://dx.doi.org/10.3390/cimb43030155 |
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author | Dai, Truong Xuan Son, Hoang Nghia Chi, Ho Nguyen Quynh Huy, Hoang Nghia Quang Minh, Nguyen Thai Tram, Nguyen Thi Thuy Huyen, Nguyen Thi Thuong Quan, To Minh Chung, Doan Chinh Nhung, Truong Hai Minh, Tran Thi Diem, Tran Hong Mai, Nguyen Thi Phuong Long, Le Thanh |
author_facet | Dai, Truong Xuan Son, Hoang Nghia Chi, Ho Nguyen Quynh Huy, Hoang Nghia Quang Minh, Nguyen Thai Tram, Nguyen Thi Thuy Huyen, Nguyen Thi Thuong Quan, To Minh Chung, Doan Chinh Nhung, Truong Hai Minh, Tran Thi Diem, Tran Hong Mai, Nguyen Thi Phuong Long, Le Thanh |
author_sort | Dai, Truong Xuan |
collection | PubMed |
description | Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca cells. However, the effects of microgravity on these cells have not been well characterized, especially in granulosa cells. This study aimed to investigate the effects of simulated microgravity (SMG) on the proliferation and morphology of porcine granulosa cells (pGCs). pGC proliferation from the SMG group was inhibited, demonstrated by the reduced O.D. value and cell density in the WST-1 assay and cell number counting. SMG-induced pGCs exhibited an increased ratio of cells in the G0/G1 phase and a decreased ratio of cells in the S and G2/M phase. Western blot analysis indicated a down-regulation of cyclin D1, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 6 (cdk6), leading to the prevention of the G1-S transition and inducing the arrest phase. pGCs under the SMG condition showed an increase in nuclear area. This caused a reduction in nuclear shape value in pGCs under the SMG condition. SMG-induced pGCs exhibited different morphologies, including fibroblast-like shape, rhomboid shape, and pebble-like shape. These results revealed that SMG inhibited proliferation and induced morphological changes in pGCs. |
format | Online Article Text |
id | pubmed-8929043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89290432022-06-04 Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells Dai, Truong Xuan Son, Hoang Nghia Chi, Ho Nguyen Quynh Huy, Hoang Nghia Quang Minh, Nguyen Thai Tram, Nguyen Thi Thuy Huyen, Nguyen Thi Thuong Quan, To Minh Chung, Doan Chinh Nhung, Truong Hai Minh, Tran Thi Diem, Tran Hong Mai, Nguyen Thi Phuong Long, Le Thanh Curr Issues Mol Biol Article Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca cells. However, the effects of microgravity on these cells have not been well characterized, especially in granulosa cells. This study aimed to investigate the effects of simulated microgravity (SMG) on the proliferation and morphology of porcine granulosa cells (pGCs). pGC proliferation from the SMG group was inhibited, demonstrated by the reduced O.D. value and cell density in the WST-1 assay and cell number counting. SMG-induced pGCs exhibited an increased ratio of cells in the G0/G1 phase and a decreased ratio of cells in the S and G2/M phase. Western blot analysis indicated a down-regulation of cyclin D1, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 6 (cdk6), leading to the prevention of the G1-S transition and inducing the arrest phase. pGCs under the SMG condition showed an increase in nuclear area. This caused a reduction in nuclear shape value in pGCs under the SMG condition. SMG-induced pGCs exhibited different morphologies, including fibroblast-like shape, rhomboid shape, and pebble-like shape. These results revealed that SMG inhibited proliferation and induced morphological changes in pGCs. MDPI 2021-12-10 /pmc/articles/PMC8929043/ /pubmed/34940129 http://dx.doi.org/10.3390/cimb43030155 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dai, Truong Xuan Son, Hoang Nghia Chi, Ho Nguyen Quynh Huy, Hoang Nghia Quang Minh, Nguyen Thai Tram, Nguyen Thi Thuy Huyen, Nguyen Thi Thuong Quan, To Minh Chung, Doan Chinh Nhung, Truong Hai Minh, Tran Thi Diem, Tran Hong Mai, Nguyen Thi Phuong Long, Le Thanh Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title | Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title_full | Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title_fullStr | Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title_full_unstemmed | Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title_short | Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells |
title_sort | simulated microgravity induces the proliferative inhibition and morphological changes in porcine granulosa cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929043/ https://www.ncbi.nlm.nih.gov/pubmed/34940129 http://dx.doi.org/10.3390/cimb43030155 |
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