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Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency

A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which...

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Autores principales: Nie, Zhi, Luo, Bowen, Zhang, Xiao, Wu, Ling, Liu, Dan, Guo, Jialei, He, Xuan, Gao, Duojiang, Gao, Shiqiang, Gao, Shibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929058/
https://www.ncbi.nlm.nih.gov/pubmed/34563050
http://dx.doi.org/10.3390/cimb43020081
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author Nie, Zhi
Luo, Bowen
Zhang, Xiao
Wu, Ling
Liu, Dan
Guo, Jialei
He, Xuan
Gao, Duojiang
Gao, Shiqiang
Gao, Shibin
author_facet Nie, Zhi
Luo, Bowen
Zhang, Xiao
Wu, Ling
Liu, Dan
Guo, Jialei
He, Xuan
Gao, Duojiang
Gao, Shiqiang
Gao, Shibin
author_sort Nie, Zhi
collection PubMed
description A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.
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spelling pubmed-89290582022-06-04 Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency Nie, Zhi Luo, Bowen Zhang, Xiao Wu, Ling Liu, Dan Guo, Jialei He, Xuan Gao, Duojiang Gao, Shiqiang Gao, Shibin Curr Issues Mol Biol Article A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize. MDPI 2021-09-13 /pmc/articles/PMC8929058/ /pubmed/34563050 http://dx.doi.org/10.3390/cimb43020081 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nie, Zhi
Luo, Bowen
Zhang, Xiao
Wu, Ling
Liu, Dan
Guo, Jialei
He, Xuan
Gao, Duojiang
Gao, Shiqiang
Gao, Shibin
Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title_full Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title_fullStr Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title_full_unstemmed Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title_short Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
title_sort combined transcriptome and proteome analysis of maize (zea mays l.) reveals a complementary profile in response to phosphate deficiency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929058/
https://www.ncbi.nlm.nih.gov/pubmed/34563050
http://dx.doi.org/10.3390/cimb43020081
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