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Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study

Previous studies assessed the involvement and impact of periodontal bacteria in preeclamptic women with chronic periodontitis. To explore further, the current study aimed to associate periodontal viruses and bacteria with mir155 levels in placental tissues of preeclamptic women with generalized chro...

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Autores principales: Mahendra, Jaideep, Mahendra, Little, Mugri, Maryam H., Sayed, Mohammed E., Bhandi, Shilpa, Alshahrani, Rahaf Turki, Balaji, Thodur Madapusi, Varadarajan, Saranya, Tanneeru, Swetha, P., Abirami Nayaki Rao, Srinivasan, Sruthi, Reda, Rodolfo, Testarelli, Luca, Patil, Shankargouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929077/
https://www.ncbi.nlm.nih.gov/pubmed/34449559
http://dx.doi.org/10.3390/cimb43020060
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author Mahendra, Jaideep
Mahendra, Little
Mugri, Maryam H.
Sayed, Mohammed E.
Bhandi, Shilpa
Alshahrani, Rahaf Turki
Balaji, Thodur Madapusi
Varadarajan, Saranya
Tanneeru, Swetha
P., Abirami Nayaki Rao
Srinivasan, Sruthi
Reda, Rodolfo
Testarelli, Luca
Patil, Shankargouda
author_facet Mahendra, Jaideep
Mahendra, Little
Mugri, Maryam H.
Sayed, Mohammed E.
Bhandi, Shilpa
Alshahrani, Rahaf Turki
Balaji, Thodur Madapusi
Varadarajan, Saranya
Tanneeru, Swetha
P., Abirami Nayaki Rao
Srinivasan, Sruthi
Reda, Rodolfo
Testarelli, Luca
Patil, Shankargouda
author_sort Mahendra, Jaideep
collection PubMed
description Previous studies assessed the involvement and impact of periodontal bacteria in preeclamptic women with chronic periodontitis. To explore further, the current study aimed to associate periodontal viruses and bacteria with mir155 levels in placental tissues of preeclamptic women with generalized chronic periodontitis. Four-hundred 45 pregnant women, 18–35 years of age, were selected and divided into four groups (controls, A, B, and C) where the Controls included 145 systemically and periodontally healthy pregnant women Group A-100 systemically healthy pregnant women with chronic periodontitis, Group B- 100 preeclamptic women with chronic periodontitis, Group C- 100 preeclamptic women without chronic periodontitis. Age, BMI, SES, and periodontal parameters such as PI, BOP, PPD, and CAL were noted. Periodontal pathogens such as Tf, Td, Pg, Pi, Fn, HSV, EBV, and HCMV were tested in subgingival plaque, placental tissues, and mir155. We observed that PI, BOP, PPD, CAL, Tf, and EBV were highly significant in Group B. We found a higher number of periodontal bacteria, viruses, and mir 155 in Group B showing a higher risk of preeclampsia. More genetic studies in this field are advised to ascertain the role of periodontopathogens and mir 155 in preeclampsia and periodontal inflammation. What is already known on this subject? Periodontal diseases pose an increased risk of developing preeclampsia and delivering preterm and/or low-birth-weight babies. What do the results of this study add? Periodontal variables such as PI, pocket depth, BOP, and clinical attachment levels, were found to be increased in the preeclamptic women with chronic periodontitis. The significant difference was seen in the relative fold expression of mir155 with higher gene expression of mir155 in groups B and A as compared to group C and controls. What are the implications of these findings for clinical practice and/or further research? In our study, mir155 correlation with the periodontal parameters and periodontal pathogens further strengthen the evidence of periodontal inflammation as a risk of preeclampsia in pregnant women especially when associated with chronic periodontitis. mir155 can be considered to be one of the genetic biomarkers and can be used as a diagnostic tool for the early detection of PE.
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spelling pubmed-89290772022-06-04 Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study Mahendra, Jaideep Mahendra, Little Mugri, Maryam H. Sayed, Mohammed E. Bhandi, Shilpa Alshahrani, Rahaf Turki Balaji, Thodur Madapusi Varadarajan, Saranya Tanneeru, Swetha P., Abirami Nayaki Rao Srinivasan, Sruthi Reda, Rodolfo Testarelli, Luca Patil, Shankargouda Curr Issues Mol Biol Article Previous studies assessed the involvement and impact of periodontal bacteria in preeclamptic women with chronic periodontitis. To explore further, the current study aimed to associate periodontal viruses and bacteria with mir155 levels in placental tissues of preeclamptic women with generalized chronic periodontitis. Four-hundred 45 pregnant women, 18–35 years of age, were selected and divided into four groups (controls, A, B, and C) where the Controls included 145 systemically and periodontally healthy pregnant women Group A-100 systemically healthy pregnant women with chronic periodontitis, Group B- 100 preeclamptic women with chronic periodontitis, Group C- 100 preeclamptic women without chronic periodontitis. Age, BMI, SES, and periodontal parameters such as PI, BOP, PPD, and CAL were noted. Periodontal pathogens such as Tf, Td, Pg, Pi, Fn, HSV, EBV, and HCMV were tested in subgingival plaque, placental tissues, and mir155. We observed that PI, BOP, PPD, CAL, Tf, and EBV were highly significant in Group B. We found a higher number of periodontal bacteria, viruses, and mir 155 in Group B showing a higher risk of preeclampsia. More genetic studies in this field are advised to ascertain the role of periodontopathogens and mir 155 in preeclampsia and periodontal inflammation. What is already known on this subject? Periodontal diseases pose an increased risk of developing preeclampsia and delivering preterm and/or low-birth-weight babies. What do the results of this study add? Periodontal variables such as PI, pocket depth, BOP, and clinical attachment levels, were found to be increased in the preeclamptic women with chronic periodontitis. The significant difference was seen in the relative fold expression of mir155 with higher gene expression of mir155 in groups B and A as compared to group C and controls. What are the implications of these findings for clinical practice and/or further research? In our study, mir155 correlation with the periodontal parameters and periodontal pathogens further strengthen the evidence of periodontal inflammation as a risk of preeclampsia in pregnant women especially when associated with chronic periodontitis. mir155 can be considered to be one of the genetic biomarkers and can be used as a diagnostic tool for the early detection of PE. MDPI 2021-07-29 /pmc/articles/PMC8929077/ /pubmed/34449559 http://dx.doi.org/10.3390/cimb43020060 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mahendra, Jaideep
Mahendra, Little
Mugri, Maryam H.
Sayed, Mohammed E.
Bhandi, Shilpa
Alshahrani, Rahaf Turki
Balaji, Thodur Madapusi
Varadarajan, Saranya
Tanneeru, Swetha
P., Abirami Nayaki Rao
Srinivasan, Sruthi
Reda, Rodolfo
Testarelli, Luca
Patil, Shankargouda
Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title_full Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title_fullStr Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title_full_unstemmed Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title_short Role of Periodontal Bacteria, Viruses, and Placental mir155 in Chronic Periodontitis and Preeclampsia—A Genetic Microbiological Study
title_sort role of periodontal bacteria, viruses, and placental mir155 in chronic periodontitis and preeclampsia—a genetic microbiological study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929077/
https://www.ncbi.nlm.nih.gov/pubmed/34449559
http://dx.doi.org/10.3390/cimb43020060
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